LOCUS       V026825                 6090 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026825
VERSION     V026825
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2237
                     /label="Pten(NM_008960)"
                     /note="Pten(NM_008960)"
                     /gene="Pten"
     CDS             2256..2285
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2304..2327
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2347..2363)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2374..2996
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3145..3733)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4062..4109
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4341..5135)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5170..5527)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5529..5633)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5660..6088
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gacagccatc atcaaagaga tcgttagcag aaacaaaagg agatatcaag
     1081 aggatggatt cgacttagac ttgacctata tttatccaaa tattattgct atgggatttc
     1141 ctgcagaaag acttgaaggt gtatacagga acaatattga tgatgtagta aggtttttgg
     1201 attcaaagca taaaaaccat tacaagatat acaatctatg tgctgagaga cattatgaca
     1261 ccgccaaatt taactgcaga gttgcacagt atccttttga agaccataac ccaccacagc
     1321 tagaacttat caaacccttc tgtgaagatc ttgaccaatg gctaagtgaa gatgacaatc
     1381 atgttgcagc aattcactgt aaagctggaa agggacggac tggtgtaatg atttgtgcat
     1441 atttattgca tcggggcaaa tttttaaagg cacaagaggc cctagatttt tatggggaag
     1501 taaggaccag agacaaaaag ggagtcacaa ttcccagtca gaggcgctat gtatattatt
     1561 atagctacct gctaaaaaat cacctggatt acagacccgt ggcactgctg tttcacaaga
     1621 tgatgtttga aactattcca atgttcagtg gcggaacttg caatcctcag tttgtggtct
     1681 gccagctaaa ggtgaagata tattcctcca attcaggacc cacgcggcgg gaggacaagt
     1741 tcatgtactt tgagttccct cagccattgc ctgtgtgtgg tgatatcaaa gtagagttct
     1801 tccacaaaca gaacaagatg ctcaaaaagg acaaaatgtt tcacttttgg gtaaatacgt
     1861 tcttcatacc aggaccagag gaaacctcag aaaaagtgga aaatggaagt ctttgtgatc
     1921 aggaaatcga tagcatttgc agtatagagc gtgcagataa tgacaaggag tatcttgtac
     1981 tcaccctaac aaaaaacgat cttgacaaag caaacaaaga caaggccaac cgatacttct
     2041 ctccaaattt taaggtgaaa ctatacttta caaaaacagt agaggagcca tcaaatccag
     2101 aggctagcag ttcaacttct gtgactccag atgttagtga caatgaacct gatcattata
     2161 gatattctga caccactgac tctgatccag agaatgaacc ttttgatgaa gatcagcatt
     2221 cacaaattac aaaagtcacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     2281 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     2341 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     2401 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     2461 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     2521 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     2581 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     2641 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     2701 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     2761 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     2821 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     2881 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     2941 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     3001 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     3901 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     3961 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     4021 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     4081 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     4141 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     4201 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     4261 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     4321 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     4381 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     4441 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     4501 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     4561 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     4621 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     4681 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     4741 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     4801 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     4861 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     4921 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     4981 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     5041 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5101 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     5161 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     5221 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     5281 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     5341 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5401 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5461 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     5521 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5641 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6061 ctgatttaac aaaaatttaa cgcgaatttt
//
