LOCUS V031147 6147 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031147 VERSION V031147 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2294 /label="Prr22(NM_001195673)" /note="Prr22(NM_001195673)" /gene="Prr22" CDS 2313..2342 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2361..2384 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2404..2420) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2431..3053 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3202..3790) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 4119..4166 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4398..5192) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5227..5584) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5586..5690) /gene="bla" /label="AmpR promoter" rep_origin 5717..6145 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gcagcatccc aaaaccctgt atcccccagc catatcccag gaaggcttca 1081 gcctgcagag cctggagggc cctgaggtgc caggtggccc agaacccctt cccaccgtag 1141 ccaatgctaa cctgcactat cagacccccg gctcggacca ggatgtgctt ccagcccctc 1201 cagcaggctt ccagatggct ccctgcggct gcttcttcga cccccgaatc taccgcatcg 1261 agtgggccac ctccgacttt ggccagtcat ccctgtacaa agtggcagtg gctgggggtc 1321 ctgccttgtc tggtggttac ttgctggagg ccccatccta cctcaaggcc ccggggccac 1381 cacctctact gtacccccac taccagccgg cccccagcgg gccacagtac ctcacacatt 1441 accttccagc ggaggagcct gtgcctgagg ctctgggctt catgcgggac ggagggcctc 1501 tcaacttcat ggagatgctc agagaccgcc tggcacctcc gccaaccccc aaagagacta 1561 cgccatctcc cttgcttatc acagtcccca cggcacacac gctgccaccc gggccctatg 1621 gccatctcgg tggccaagcc agccagttcc caggacccca ggtgaccatg aggcccattg 1681 aggcttccag ggagctgcag agcagtggtg tggccaggcc aggtctgcga ttcccacccg 1741 ggcctgtaga gcccaaggtg gccaaggtgg aggacgtcac cccagtgggc tcaggggaga 1801 ccatgcctgc cgaggcggca cgtgcgttct tcctaccgga caaggtcctt ctcgaagatg 1861 ccatgaaact ttttgattgt ctccctggtg gcactgagcc tgaggtggcc ttgcacaggg 1921 gtcctggccc tggactgcgg gacagtggtg gcggtgggga tgacttccca accgacattc 1981 gctccctgca cctgccagat gacctgctgt cctttgacta cagtgtcccc gagatcctag 2041 atgcggtggc caatgtggac tactttttca gcttcaaggc cctggatgat gaaccaccag 2101 tgccgcatct cggggtccct gccactgaca cagtggcccc aggcctgcgg tcccatcaac 2161 tggggaaaaa acccagcatg cccaccaaga aagggaagcc aggcagcaga cacaggcaga 2221 ccacaggccc agctgacaca gctgccgccg ccgctgctgc aggacccagg ctggacccgg 2281 gagccatccc caatacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 2341 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 2401 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 2461 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2521 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2581 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2641 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2701 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 2761 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2821 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 2881 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 2941 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 3001 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 3061 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3121 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3181 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3241 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3301 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3361 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3421 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3481 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3541 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3601 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3661 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3721 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3781 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 3961 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 4021 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 4081 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 4141 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 4201 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 4261 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 4321 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 4381 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 4441 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 4501 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 4561 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 4621 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 4681 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 4741 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 4801 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 4861 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 4921 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 4981 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 5041 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 5101 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 5161 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 5221 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 5281 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 5341 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 5401 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 5461 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 5521 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 5581 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5701 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 6121 atttaacaaa aatttaacgc gaatttt //