LOCUS       V031147                 6147 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031147
VERSION     V031147
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2294
                     /label="Prr22(NM_001195673)"
                     /note="Prr22(NM_001195673)"
                     /gene="Prr22"
     CDS             2313..2342
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2361..2384
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2404..2420)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2431..3053
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3202..3790)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4119..4166
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4398..5192)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5227..5584)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5586..5690)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5717..6145
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcagcatccc aaaaccctgt atcccccagc catatcccag gaaggcttca
     1081 gcctgcagag cctggagggc cctgaggtgc caggtggccc agaacccctt cccaccgtag
     1141 ccaatgctaa cctgcactat cagacccccg gctcggacca ggatgtgctt ccagcccctc
     1201 cagcaggctt ccagatggct ccctgcggct gcttcttcga cccccgaatc taccgcatcg
     1261 agtgggccac ctccgacttt ggccagtcat ccctgtacaa agtggcagtg gctgggggtc
     1321 ctgccttgtc tggtggttac ttgctggagg ccccatccta cctcaaggcc ccggggccac
     1381 cacctctact gtacccccac taccagccgg cccccagcgg gccacagtac ctcacacatt
     1441 accttccagc ggaggagcct gtgcctgagg ctctgggctt catgcgggac ggagggcctc
     1501 tcaacttcat ggagatgctc agagaccgcc tggcacctcc gccaaccccc aaagagacta
     1561 cgccatctcc cttgcttatc acagtcccca cggcacacac gctgccaccc gggccctatg
     1621 gccatctcgg tggccaagcc agccagttcc caggacccca ggtgaccatg aggcccattg
     1681 aggcttccag ggagctgcag agcagtggtg tggccaggcc aggtctgcga ttcccacccg
     1741 ggcctgtaga gcccaaggtg gccaaggtgg aggacgtcac cccagtgggc tcaggggaga
     1801 ccatgcctgc cgaggcggca cgtgcgttct tcctaccgga caaggtcctt ctcgaagatg
     1861 ccatgaaact ttttgattgt ctccctggtg gcactgagcc tgaggtggcc ttgcacaggg
     1921 gtcctggccc tggactgcgg gacagtggtg gcggtgggga tgacttccca accgacattc
     1981 gctccctgca cctgccagat gacctgctgt cctttgacta cagtgtcccc gagatcctag
     2041 atgcggtggc caatgtggac tactttttca gcttcaaggc cctggatgat gaaccaccag
     2101 tgccgcatct cggggtccct gccactgaca cagtggcccc aggcctgcgg tcccatcaac
     2161 tggggaaaaa acccagcatg cccaccaaga aagggaagcc aggcagcaga cacaggcaga
     2221 ccacaggccc agctgacaca gctgccgccg ccgctgctgc aggacccagg ctggacccgg
     2281 gagccatccc caatacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     2341 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc
     2401 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg
     2461 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2521 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2581 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2641 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2701 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     2761 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     2821 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     2881 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     2941 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     3001 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     3061 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3121 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3181 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3241 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3301 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3361 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3421 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3481 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3541 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3601 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3661 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3721 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3781 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     3961 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     4021 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     4081 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     4141 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     4201 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     4261 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     4321 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4381 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     4441 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     4501 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc
     4561 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     4621 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     4681 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     4741 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     4801 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     4861 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     4921 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     4981 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     5041 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     5101 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     5161 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     5221 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     5281 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     5341 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     5401 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5461 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5521 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     5581 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5701 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6121 atttaacaaa aatttaacgc gaatttt
//
