LOCUS       V031283                 8532 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031283
VERSION     V031283
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4679
                     /label="Polg(NM_017462)"
                     /note="Polg(NM_017462)"
                     /gene="Polg"
     CDS             4698..4727
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4746..4769
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4789..4805)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4816..5438
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5587..6175)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6504..6551
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6783..7577)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7612..7969)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7971..8075)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      8102..8530
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagccgcctg ctctggaaga aggtggctgg cgccaaggtc gcctcagggc
     1081 cagtaccagc aaccgcgcgc tgggtctcca gctccgtcct cgaccccgtc cccagcgacg
     1141 ggcggccgcc gtcgcaaatg ccctcctccg agaatgggca gctgcggctc aaccctctgc
     1201 tcatccagat gttgtcgaga ggcctgcacg agcagatctt cgggtgcggc ggggaaatgc
     1261 ccgacgaggc cgcggtgcag cgcagcgtag agcacctgca gaagcacggg ctctgggggc
     1321 agccggccac gcccttgcca gacgtggagc tgcgcctgcc ccggctcttc gggggcaacc
     1381 tggaccagca cttccgcctc ctggcccaga agcagagcct gccttacttg gaggcggccg
     1441 cctcgttatt ggaggcccaa ttgccccccg agcccaagag ctgggcctgg gcggagggct
     1501 ggacccggta cggccccgag ggggaggccg aacccgtggc catccccgag gagcgggccc
     1561 tggtgttcga cgtggaggtc tgcttggcag agggaacctg ccccactttg gcggtggcca
     1621 tatccccctc ggcctggtat tcctggtgca gccggcggct ggtggaagag cgttactctt
     1681 ggaccagcca gctatcgccg gctgacctaa tccctttggg gggctccact agtgccagca
     1741 gctccaccaa gcaggatggg caggaacagt tagtggtggg gcacaatgtt tcctttgacc
     1801 gagcccatat cagggaacag tatctgattc aggactcccg gatgcgtttt ctcgatacta
     1861 tgagcatgca catggccatc tcggggctga gcagcttcca gcgcagcctg tggatgggag
     1921 ccaagcaggg aaagcacaag acccagcagt ccacaaagcg agggcagaag tccccgagga
     1981 aagccaatgg tccagcgatc tcatcttggg actggatgga tatcagcagt gccaataatc
     2041 ttgcagatgt gcacaacctt tatgtggggg gacctccctt agagaaggag cctcgggagc
     2101 tgttcgtcaa aggcagcatg agggatatcc gagagaactt ccaggatctg atgcagtact
     2161 gcgcccgtga tgtgtgggcc acctttgagg ttttccagca gcagctgcca ctcttcttgg
     2221 agaggtgtcc ccacccagtg actctggctg gcatgctgga gatgggtgtg tcctacctgc
     2281 ctgtcaacca gaactgggag cgttacctga cagaggcaca gaacacatat gaggagctcc
     2341 agcgggagat gaagaagtcg ctgatggatc tggctaatga tgcctgtcag ttgctctcag
     2401 gagagaggta caaagaagac ccttggctct gggacctaga atgggatttg caggagttta
     2461 agcagaaaaa ggcaaagaag gtgaagaagc cagcctcagc cagcaagttg cccatcgagg
     2521 gagctgggcc ctttggggat cccatggatc aggaagatcc tggcccgccc agcgaggagg
     2581 aggagcttca gcgaagtgta acagcccaca accggttaca gcagctgagg agcaccacgg
     2641 acctcctgcc taagcgaccc cagcaccttc caggacaccc tgggtggtac cggaagctct
     2701 gccctcgact agatgaccct gcctgggctc ccggccccag cctcctcagc ctgcaaatgc
     2761 gggtcactcc taagctcatg gcactgacct gggatggttt tcctctacac tactcagact
     2821 ctcatggttg gggctaccta gtgcctgggc gtcgggataa tctgaccgag ccgccagtga
     2881 gccccactgt agaatccgct gcagtaacct gcccctacag agccatcgag tccttataca
     2941 ggaagcactg ccttgaacag gggaagcagc aattggagcc ccaggaggta gacctggctg
     3001 aggagttctt actcactgac agtagtgcca tgtggcaaac ggtagaagaa ctgggctgct
     3061 tagacgtaga ggctgaggcc aagatggaga attcagggct gagccaacct ctagttctgc
     3121 ctgctgcttg tgcccccaaa tccagccagc ccacgtatca ccatggcaac ggaccttata
     3181 atgatgtgaa tatccctggt tgctggtttt tcaagctgcc tcacaaggac ggtaacaact
     3241 acaatgtggg cagtcccttt gccaaagatt tcctgcccaa gatggaggat ggcaccctgc
     3301 aggctggccc aggaggtgcc agtgggcctc gtgccctgga aataaataaa atgatttctt
     3361 tttggaggaa tgctcataaa cgtatcagtt cccagatggt ggtatggctc cccaggtcag
     3421 ctctgccccg ggttgtgacc aggcacccca gcttcgatga ggaaggccac tatggggcca
     3481 tcctacccca ggtggtgact gctggcacca tcacccgtcg agctgtggag cccacgtggc
     3541 tcactgccag taatgctcgg cctgaccggg taggcagtga gttgaaagcc atggtgcagg
     3601 ctccacctgg ctatgtcctt gtgggtgctg atgtggactc acaggaactg tggatcgcag
     3661 ctgtacttgg agatgctcac tttgctggga tgcatggctg cacggccttt ggctggatga
     3721 ctctgcaggg caggaagagc agaggcactg atctgcacag caagacagct gccactgtgg
     3781 gcatcagccg agagcatgcc aaaatcttca actatggccg catctatggg gctgggcagt
     3841 cctttgctga gcgcctactg atgcagttca accacaggct cacaaggcag gaggcagctg
     3901 agaaggccca gcagatgtat gcagtcacaa aaggcctgcg ccggtatcgg ctgtctgcgg
     3961 atggtgagtg gctggtgaaa cagttgaatc ttcctgtgga caggacagag gacggctggg
     4021 tctccctaca agatcttcga atgatccgaa gagaagcttc aaggaagtca cgatggaaga
     4081 agtgggaggt agcctctgaa cgagcatgga cagggggcac agagtcagaa atgtttaata
     4141 agctggagag tattgccatg tctgatacac cacgtacccc agtactgggc tgctgcatca
     4201 gcagagcctt ggagccctca gttgtccagg gagagtttat aaccagtcgt gtgaactggg
     4261 tggtacagag ctctgctgta gactacttac atctcatgct tgtggccatg aagtggctgt
     4321 ttgaggaatt tgccattgat gggcgcttct gcatcagcat ccacgacgag gttcgctacc
     4381 tggtgcgtga ggaggaccgc taccgtgccg ccctggcact gcagatcacc aatctcctga
     4441 ccaggtgcat gtttgcctat aagctgggtc tgaatgatct gccccagtca gtcgcctttt
     4501 tcagtgcagt agacattgac cagtgcctca ggaaggaagt gaccatggac tgtaaaactc
     4561 cttctaaccc aactgggatg gaaaggagat atgggatccc ccagggtgaa gcactggata
     4621 tttaccagat aattgaactc accaaaggct ccttggaaaa acgaagccag cctggaccca
     4681 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     4741 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     4801 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     4861 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4921 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4981 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     5041 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     5101 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     5161 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     5221 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     5281 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     5341 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     5401 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     5461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5761 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6001 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6301 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     6361 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     6421 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     6481 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     6541 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     6601 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     6661 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     6721 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     6781 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     6841 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     6901 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     6961 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     7021 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     7081 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     7141 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     7201 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     7261 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     7321 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     7381 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     7441 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     7501 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     7561 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     7621 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7681 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7741 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7801 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     7861 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     7921 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     7981 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8101 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8161 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8221 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8281 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8341 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8401 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8461 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8521 aacgcgaatt tt
//
