LOCUS       V030942                 6558 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030942
VERSION     V030942
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2705
                     /label="Pdpk1(NM_011062)"
                     /note="Pdpk1(NM_011062)"
                     /gene="Pdpk1"
     CDS             2724..2753
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2772..2795
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2815..2831)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2842..3464
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3613..4201)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4530..4577
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4809..5603)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5638..5995)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5997..6101)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6128..6556
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggccaggacc accagccagc tgtatgacgc tgtgcccatt cagtccagtg
     1081 tggtgctatg ttcctgccca tccccatcaa tggtgaggtc ccagactgag cccggttcgt
     1141 cccctggcat tcctagtggt gttagcaggc agggatccac catggatggc accacagctg
     1201 aagcccgacc aagcaccaac cccttgcagc agcaccctgc ccagctgcca ccacagcctc
     1261 gcaagaaacg ccctgaagac ttcaagtttg ggaaaattct tggcgagggc tctttttcaa
     1321 cagttgttct ggcccgagaa ctggccactt ccagagaata tgctattaaa attctggaga
     1381 aacgtcatat tataaaagaa aacaaagttc cgtatgtaac tagagagaga gatgtgatgt
     1441 cacgcctgga tcaccccttc tttgtgaaac tttattttac atttcaggac gacgaaaagc
     1501 tgtattttgg ccttagttat gccaaaaatg gagagctact taaatacatc cgcaaaattg
     1561 gctcatttga tgagacctgt acccggtttt acacggctga gattgtgtct gctttagagt
     1621 acttgcatgg caagggcatc attcacagag accttaaacc agaaaacatt ttgttaaatg
     1681 aagacatgca catccagatc acagattttg gaacagcaaa agtgttatcc ccagagagca
     1741 aacaagccag ggccaactca tttgtaggaa cagcacagta tgtttctcca gagctgctca
     1801 cagagaagtc ggcgtgtaaa agttcagacc tttgggccct tggatgtata atctatcagc
     1861 tcgtggcagg actcccacca ttcagagccg ggaatgaata tcttatattt cagaagatca
     1921 ttaagctgga atatcatttc ccagaaaaat tcttccctaa ggctagagat cttgtggaaa
     1981 aactcttggt tttagatgcc acaaagcgtt taggctgtga agagatggaa gggtacgggc
     2041 ctctcaaagc tcatccattc tttgagacca tcacttggga gaatttgcac cagcagacac
     2101 ctccgaagct cacagcttac ctaccagcca tgtcagagga tgatgaagac tgctatggca
     2161 actacgacaa tctcctgagc cagtttggct tcatgcaggt gtcatcctcc tcctcttccc
     2221 actccctgtc tacggtggaa accagcctgc cccagaggtc gggcagcaac atagagcagt
     2281 acatccatga tttggacact aactcttttg aactagactt acagttttca gaagatgaaa
     2341 aaaggttgtt attggaaaag caagccggtg gaaacccttg gcaccagttt gtagaaaata
     2401 atctaatatt aaaaatgggt ccagtggata agcgaaaggg tttatttgca agacgacgac
     2461 agttattact cacagaaggg ccacatttat attatgttga tcctgtcaac aaggtcttga
     2521 aaggtgaaat cccatggtca caagaactcc gaccagaagc caagaatttt aaaactttct
     2581 ttgtccacac gcctaacagg acgtactacc tgatggatcc aagcgggaat gctcacaagt
     2641 ggtgcagaaa gatccaggag gtttggaggc agcagtacca gagcaatcca gatgctgctg
     2701 tgcagacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     2761 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     2821 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     2881 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     2941 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3001 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3061 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3121 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3181 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3241 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3301 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     3361 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     3421 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     3481 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4321 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     4381 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     4441 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     4501 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     4561 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4621 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     4681 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     4741 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     4801 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4861 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4921 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     4981 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     5041 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     5101 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     5161 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     5221 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     5281 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5341 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5401 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5461 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     5521 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5581 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     5641 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     5701 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     5761 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     5821 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5881 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5941 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     6001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6061 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6121 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6181 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6241 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6301 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6361 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6421 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6481 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6541 aaatttaacg cgaatttt
//
