LOCUS       V046565                11661 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046565
VERSION     V046565
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6341
                     /label="Pdcd11(NM_011053)"
                     /note="Pdcd11(NM_011053)"
                     /gene="Pdcd11"
     CDS             6348..6413
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6437..7025
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7091..7139
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7341..7769
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7783..8112
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8179..8973
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9149..9270
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9319..9335)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9343..9359
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9367..9397)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9412..9433
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9721..10309)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10480..11340)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11341..11445)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc aaacctggag gaaagcttcc
      781 cccgaggggg gacacggaaa ctccacaaat cagagaagtc ttcccagcag gtggttgaac
      841 aagacaactt atttgatgtt tccacagaag aaggtcccat taaaaggaaa aagagccaga
      901 aagggccagc aaaaacaaaa aaattgaaaa ttgaaaagag aaaatccatc aaatctataa
      961 aagagaagtt tgaaattctt agtcttgagt ccctatgtga ggggatgcgg attttgggct
     1021 gtgtgaagga ggtgagtgag ctggagctgg tggtcagcct tcccaacggc ctccaagggt
     1081 ttgtgcaagt gaccgaagtc tgtgacgcct acactcagaa gctgaatgag caggtggcac
     1141 aagaagaacc tctggaggac ctgctccgcc tgcctgagct cttctcccct ggaatgttgg
     1201 tgaggtgtgt ggtgagcagc ctggatgtca cagagagtgg caagaagagc gtcaagcttt
     1261 ctgtgaaccc caaacgtgtc aacaaagtgc tgagtgctga tgccctgagg cctggcatgc
     1321 tgctgacagg cacagtgtca agcctggaag accatggcta cctagtggac attggcgttg
     1381 gtgggaccag agcttttctg tcacttaaga aggcacaaga gtacatccgg cagaagaaca
     1441 aaggtgctaa gttcaaagtc ggccagtacc tgacctgtgt ggttgaagag gtgaaaagca
     1501 acggaggagt tgtgagtctc tctgtagagc actccgaggt ttcctctgcc ttcgcaactg
     1561 aggagcaaag ctggaacctg aataacttac taccagggct gctggtcaaa gcccaggtgc
     1621 agaaggtaac tcagtttgga ctccagctaa atttccttac gttcttcaag ggcctggttg
     1681 acttcatgca tctggagccc aagaaaatgg gatcttattc ttcaaatcaa acggtaaaag
     1741 cctgcatcct ctgcgtccat cctcgcacca gagttgtgcg actgagcctg cgccccatct
     1801 tcctgcatcc tgggcgtcca ctcacccgca tctcttacca gcagctaggg gctgtgctag
     1861 atgatgtccc tgttcagggt ttttttaaga atgcgggggc catcttcagg ctgaaggatg
     1921 gggtactggc ctacgcccgg gtcagccatc tctctgattc taagaaagca ttcaacgctg
     1981 aggccttcaa accggggagc actcacaagt gtagaatcat tgactacagc cagatggatg
     2041 agctggcctt gctctctctg cggaaatcaa ttattgcagc tccattcctt agatatcatg
     2101 acatcaaaat agggacagta gtaaagggca cagtgctggc cataaagcca tttgggatcc
     2161 tggtgaaggt gggtgagcag ataaaaggcc tggtgccctc catgcacctg gctgacatca
     2221 tgatgaagaa cccagagaag aagtacagcc caggggatga ggtcaagtgc agggttttgc
     2281 tctgtgaccc tgaagccaag aagctaatta tgaccctgaa aaaaaccctt gtcacatcca
     2341 aactgtctct catcacctgc tatgagggtg ccaagcctgg cctgcagacg cacggtgtta
     2401 tcatacgggt caaggactat ggctgcattg tgaagttcta caacgatgtg caggggctag
     2461 tgcctaagca tgagcttagc acccagcaca tcccggaccc agagacggtc ttttacactg
     2521 gccaggtggt aaaggtggca gtgctgagct gtgagccttc caaggagagg atgctcctgt
     2581 ccttcagact gttgagtgac tcgcggccaa aggatccggg tgttgagagc agtcagaaga
     2641 aaacaggagc tgtgcgcatc gggcagttgg tggatgtgaa ggtcctggag aagaccaaaa
     2701 ccgggctgga ggtggccatc ctgccccata acacccctgc tttcctcccc acgccacacc
     2761 tgtccgacca cgctgccaat ggcccactgt tgcaccactg gctccagaca ggtgacaccc
     2821 ttcaccgagt tctgtgtttg agccagagtg agagacacat tcttctttgc aggaaaccag
     2881 ctttggtctc cacagtagaa ggtggccagg accccaagag cctctcagaa atccagcccg
     2941 gaatgttgct cattggtttt gtgaagtgca tcaaggagta tggcgtgttc gtccagttcc
     3001 cctcaggtct cagtggactg tcccccaaaa ctatcatgag tgacaagttc gtaaccactc
     3061 caagtgaaca ctttgtggag ggtcagacag tcgttgctaa ggtgaccaat gtggatgaat
     3121 cgaagcagag gatgctgctg tctctgcggc tgtcagactg cagtctgggg gattcggcca
     3181 gcaccagctt cctcctcctg tgccagtgcc tggaagagct gcagggcatt cgcagcctca
     3241 tgagcaacca agactctgta ttgatccaga cacttgctga catgaccccg gggatggtcc
     3301 ttgatgccgt ggtccatgag gtgttagaag atggttcagt ggtgttcagt agtgatcctg
     3361 tgcctgacct ggtcctgaga gccagcagat accaccgggc agggcaggag gtggagcctg
     3421 gacagaagaa gaaggtcgtg gttctacacg tggacatgtt gaagttggag gtccacgtct
     3481 cccttcacca ggacttggtg aacaggaaaa cgagaaagct gagaaaaagc agtagacacc
     3541 agggaatcgt acagcatctg gaagagtcct ttgccgtggc atccttggtg gagactggcc
     3601 accttgtggc tttctctctg atctctcacc tcaatgatac cttccacttt gactctgaga
     3661 agctgcgtgt ggggcagggt gtctgcctta ccctcaagac cacagaaccg ggagtgaccg
     3721 gtctgatttt ggctgtggaa gggccagctt ctaagaggac caggatgcca gttcagaggg
     3781 attcagagac agttgatgac aaaggagagg agaaagagga ggaggaggag gaggaggaga
     3841 aggaagaaga gaacttgact gtgaagtcta agaaaaggca tagcctggct attggggaca
     3901 aagtcactgg gaccattaag gctgtgaagg ccacacatgt tgttgtaacc ctagctgatg
     3961 gctttgttgg ctgtatccat gcctcccgca tcttagatga tgtcccagtg ggcacctcgc
     4021 ctacgaccac actgaaagcc gggaagaaag tcactgcgag agtgatcggt gggcgagatg
     4081 tgaagacatc taagtttctc ccaataagtc accccagatt tgttctgacc atcctggagc
     4141 tgagtgttcg gccaagtgag ttgaagggta gctacagtgc tcttaatact cactctgaga
     4201 gccccgtgga gaagatcagg cagtaccagg ctgggcagac tgtgacctgc ttcttcaaga
     4261 agtacaacgt gatgaagaag tggcttgaag tggacatcgg accggacatc cgagggagaa
     4321 ttcccttgtt gctcacttcc ctcagcttca aggttctgaa acacccagac aagaagttcc
     4381 aggttgggca ggccatcgag gccaccgttg ttgacccaga cgtccccagg gctttcttat
     4441 gtctgtcact cataggacct tacagacttg aagaaggtga agtggccatg ggccgagtga
     4501 tgaaggttgt acccaacagg ggactgaccg tctccttccc ttttgggaag atagggaaag
     4561 tcagtatgtt tcacctgagc gactcgtact ccgaggcacc cctggaggac ttctgccccc
     4621 agaagattgt cagatgttac atcctgtcca cagcacaccg tgtgctggct ttgtcactcc
     4681 ggtcatccag gacaaacaga gagacaaaaa acagaataga agatccagaa atcaactcca
     4741 ttgaggacgt gaaggaaggg cagctcctgc ggggctatgt gaaatgtgtc ctgccaagca
     4801 gtgtgatcat aggccttgga ccttcagttc tgggtttggc caagtattca catgtctctg
     4861 agtgtgtccc accagagaaa gagctttata acggatgcct ccctgaggga aagctggtca
     4921 ctgccaaggt cctgcgcgtg aaccccatga agaacctgat agagctgtcg ctccttccca
     4981 gcgacactgg gaggccagac gtgttctctc ctgctccaga accaaaacaa gaggagagga
     5041 gcggaggggc tgaggagggc cagaaaagga aagagaagaa ccagaagaga cgggaggaga
     5101 aggaagagcc ccagaagtca cagaggggtg ggcgaggcaa gcgggagcgc caggagtctg
     5161 agagcgagca ggaactagtg aacaagaggc caaagaagtc tggagccgca gaagaagacg
     5221 acagtggtgt ggaagtgtac taccgggaag gagaggatga ggtgggggag ccgaagctgc
     5281 cacccagggg gaagcagaca aaatccacag aggtgccccg gctgcatctc tcttcaggct
     5341 tcctttggga tgtggggctt gactctctga ctccagcctt gccactccga gaagagagct
     5401 cagacagcga ggatgagcag ccgcaccaag ctaagaagaa gaagggcaag aaggagaggg
     5461 agctggagaa gcagaaggca gagaaggagc tgtcgcgcat tgaggaggca ctcatggacc
     5521 ctgggcgaca gccagagtct gcggatgact ttgaccggct ggtgctaagc tctcccaaca
     5581 gctccatcct ttggctgcag tacatggctt tccacctaca ggccacggag atcgagaagg
     5641 cccgggctgt ggctgagagg gccctgaaga ccatctcatt cagagaggag caggagaagc
     5701 tgaacgtgtg ggtggccctg ctgaacctgg aaaacatgta cggctctcag gagtccctga
     5761 ctaaggtctt cgagcgagct gtgcagtaca acgagcctct caaggttttc ctgcatctgg
     5821 ctgacatcta caccaagtcc gagaaataca aggaagctgg tgagctgtac aaccggatgc
     5881 tgaaacggtt ccggcaggag aaggctgtct ggatcaaata tggtgccttt gttttgggga
     5941 ggagtcaggc tggggccagt caccgagtgc tgcagcgagc cctggagtgc ctgcccgcca
     6001 aggaacatgt ggatgtgatc gtcaagtttg cccagctgga gttccagctc ggggacgtgg
     6061 agcgcgctaa agccattttt gaaaacacac tgagcaccta ccccaaacgc acagacgtct
     6121 ggtcagtcta catcgacatg accatcaagc acggcagcca gacagcagtc agggacatct
     6181 ttgagcgggt cattcacctg agcctggcgc ctaagaggat gaagttcttt ttcaagcgct
     6241 acctggacta tgagaagcag cacggcacgg agaaggacgt gcaggcggtc aaggccaagg
     6301 ccttggagta tgtggaggcc aagagctcgg cgctggagga cggatccgac tacaaagacc
     6361 atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac aagtagtgag
     6421 ggcccgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6481 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6541 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6601 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6661 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6721 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6781 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6841 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6901 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6961 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7021 cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     7081 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     7141 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     7201 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     7261 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg
     7321 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7381 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7441 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
     7501 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7561 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7621 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7681 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga
     7741 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7861 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7921 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8041 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8101 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     8161 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8221 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8281 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8341 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8401 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8461 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8521 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8581 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8641 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     8701 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     8761 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     8821 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     8881 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     8941 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac
     9001 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     9061 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9121 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9241 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9301 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9481 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9901 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11461 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt
    11521 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    11581 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
    11641 tgcatgaaga atctgcttag g
//
