LOCUS       V031140                 5814 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031140
VERSION     V031140
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1961
                     /label="Or6b1(NM_001356410)"
                     /note="Or6b1(NM_001356410)"
                     /gene="Or6b1"
     CDS             1980..2009
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2028..2051
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2071..2087)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2098..2720
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2869..3457)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3786..3833
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4065..4859)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4894..5251)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5253..5357)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5384..5812
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggatgtggac aaccagacac gggtcaccaa gttcattctg gtggggtttc
     1081 ctgggagctt gagcatgaga gcagctgtat tcctgatgtt tcttgtggcc tatattctga
     1141 ctgtagctga aaacgtgatc atcatcctac tggtgcaaca gaaccgacca ctacacaagc
     1201 ctatgtactt cttccttgcc aacctgtctt tcttggagac ctggtacatc tctgttaccg
     1261 tgcccaagct gctgttcagc ttctggtcca tgagtaacag catttctttc actcactgta
     1321 tgatacagct ttacttcttc attgcactta tgtgtacgga atgcgttctc ctagctgcaa
     1381 tggcctatga tcgttatgtg gctatctgcc gcccattaca ctaccccacc atcatgagcc
     1441 atggactctg tttccgccta gctcttggtt cctgggtcat tggctttggc atctctttgg
     1501 caaagatcta cttcatctcc cgcctcagct tctgtggccc caatgtcatc aaccacttct
     1561 tttgtgatat ctctcccgtt cttaatctct cctgcacaga catgtccatt gcagagctgg
     1621 tggactttgt cttggctctg gtcatcttcc tctttccgct gtcaatcact gttttatcat
     1681 acggatgcat tctggccact gtgttacgca tgccaacagg aaagcagaaa gccttttcca
     1741 cctgtgcttc ccacctcgtg gtggttacca tcttctactc agccactatt tttatgtatg
     1801 cccgaccccg agctatccat gccttcaaca tgaacaaagt tatttctatc ttctatgcca
     1861 ttgtgacccc tgctctcaat cctttcattt attgcctaag gaaccgagag gtaaaagaag
     1921 ctctaaagaa attgatttat tgccaggtca tccgatctga cacgcgtacg cggccgctcg
     1981 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     2041 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg
     2101 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     2161 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     2221 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     2281 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     2341 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     2401 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     2461 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     2521 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     2581 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc
     2641 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact
     2701 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa
     2761 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3481 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3541 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3601 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     3661 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     3721 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     3781 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc
     3841 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc
     3901 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     3961 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
     4021 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
     4081 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     4141 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     4201 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     4261 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     4321 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     4381 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     4441 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     4501 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     4561 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     4621 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     4681 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     4741 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     4801 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     4861 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     4921 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     4981 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     5041 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     5101 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     5161 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     5221 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
     5281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     5401 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5461 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5521 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5581 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5641 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5701 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5761 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt
//
