LOCUS       pEnCMV-OAT(human)-HA    7363 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036801
VERSION     V036801
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2022
                     /label="OAT(NM_000274)"
                     /note="OAT(NM_000274)"
                     /gene="OAT"
     CDS             2029..2055
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2059..2085
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2089..2115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2139..2727
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    2793..2841
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3043..3471
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3485..3814
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3881..4675
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4851..4972
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5021..5037)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5045..5061
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5069..5099)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5114..5135
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5423..6011)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6182..7042)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7043..7147)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt ttccaaacta
      721 gcacatttgc agaggtttgc tgtacttagt cgcggagttc attcttcagt ggcttctgct
      781 acatctgttg caactaaaaa aacagtccaa ggccctccaa cctctgatga catttttgaa
      841 agggaatata agtatggtgc acacaactac catcctttac ctgtagccct ggagagagga
      901 aaaggtattt acttatggga tgtagaaggc agaaaatatt ttgacttcct gagttcttac
      961 agtgctgtca accaagggca ttgtcacccc aagattgtga atgctctgaa gagtcaagtg
     1021 gacaaattga ccttaacatc tagagctttc tataataacg tacttggtga atatgaggag
     1081 tatattacta aacttttcaa ctaccacaaa gttcttccta tgaatacagg agtggaggct
     1141 ggagagactg cctgtaaact agctcgtaag tggggctata ccgtgaaggg cattcagaaa
     1201 tacaaagcaa agattgtttt tgcagctggg aacttctggg gtaggacgtt gtctgctatc
     1261 tccagttcca cagacccaac cagttacgat ggttttggac catttatgcc gggattcgac
     1321 atcattccct ataatgatct gcccgcactg gagcgtgctc ttcaggatcc aaatgtggct
     1381 gcgttcatgg tagaaccaat tcagggtgaa gcaggcgttg ttgttccgga tccaggttac
     1441 ctaatgggag tgcgagagct ctgcaccagg caccaggttc tctttattgc tgatgaaata
     1501 cagacaggat tggccagaac tggtagatgg ctggctgttg attatgaaaa tgtcagacct
     1561 gatatagtcc tccttggaaa ggccctttct gggggcttat accctgtgtc tgcagtgctg
     1621 tgtgatgatg acatcatgct gaccattaag ccaggggagc atgggtccac atacggtggc
     1681 aatccactag gctgccgagt ggccatcgca gcccttgagg ttttagaaga agaaaacctt
     1741 gctgaaaatg cagacaaatt gggcattatc ttgagaaatg aactcatgaa gctaccttct
     1801 gatgttgtaa ctgccgtaag aggaaaagga ttattaaacg ctattgtcat taaagaaacc
     1861 aaagattggg atgcttggaa ggtgtgtcta cgacttcgag ataatggact tctggccaag
     1921 ccaacccatg gcgacattat caggtttgcg cctccgctgg tgatcaagga ggatgagctt
     1981 cgagagtcca ttgaaattat taacaagacc atcttgtctt tcctcgagta cccatacgac
     2041 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     2101 gtcccagact acgcatagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa
     2161 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2221 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2281 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2341 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2401 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2461 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2521 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     2581 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2641 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2701 ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa
     2761 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     2821 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     2881 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     2941 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     3001 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3061 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3121 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3181 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg
     3241 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3301 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3361 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt
     3421 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3481 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3541 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3721 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3781 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3841 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     3901 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3961 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4021 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4081 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4141 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4201 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4261 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4321 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4381 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4441 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4501 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4561 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4621 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4681 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     4741 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     4801 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     4861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4981 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5041 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5101 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5161 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5221 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5281 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5341 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5401 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5461 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5521 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5581 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     5641 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5701 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5761 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5821 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     5881 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5941 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6061 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6181 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6241 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6361 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6421 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6481 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6541 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6661 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6721 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6781 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6841 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6961 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7021 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7141 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7201 tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     7261 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     7321 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
