LOCUS       V026950                 7729 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026950
VERSION     V026950
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2385
                     /label="Nr5a2(NM_030676)"
                     /note="Nr5a2(NM_030676)"
                     /gene="Nr5a2"
     CDS             2392..2481
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2505..3093
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3159..3207
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3409..3837
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3851..4180
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4247..5041
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5217..5338
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5387..5403)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5411..5427
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5435..5465)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5480..5501
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5789..6377)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6548..7408)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7409..7513)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc tgctagtttg
      721 gatactggag attttcaaga atttcttaag catggactta cagctattgc gtctgcacca
      781 gggtcagaga ctcgccactc ccccaaacgt gaggaacaac tccgggaaaa acgtgctggg
      841 cttccggacc gacaccgacg ccccattccc gcccgcagcc gccttgtcat gctgcccaaa
      901 gtggagacgg aagccccagg actggtccga tcgcatgggg aacaggggca gatgccagaa
      961 aacatgcaag tgtctcaatt taaaatggtg aattactcct atgatgaaga tctggaagag
     1021 ctatgtcctg tgtgtggcga taaagtgtct gggtaccatt acggtctcct cacgtgcgaa
     1081 agctgcaagg gtttttttaa gcgaactgtc caaaaccaaa aaaggtacac gtgcatagag
     1141 aaccagaatt gccaaattga caaaacgcag agaaaacgat gtccctactg tcgattcaaa
     1201 aaatgtatcg atgttgggat gaagctggaa gccgtaagag ccgaccgcat gcgagggggc
     1261 agaaataagt ttgggccaat gtacaagaga gacagggctt tgaagcagca gaagaaagcc
     1321 ctcattcgag ccaatggact taagctggaa gccatgtctc aggtgatcca agcaatgccc
     1381 tcagacctga cctctgcaat tcagaacatt cattccgcct ccaaaggcct acctctgagc
     1441 catgtagcct tgcctccgac agactatgac agaagtccct ttgtcacatc tcccattagc
     1501 atgacaatgc cacctcacag cagcctgcat ggttaccaac cctatggtca ctttcctagt
     1561 cgggccatca agtctgagta cccagacccc tactccagct cacctgagtc aatgatgggt
     1621 tactcctaca tggatggtta ccagacaaac tccccggcca gcatcccaca cctgatactg
     1681 gaacttttga agtgtgaacc agatgagcct caagttcaag cgaagatcat ggcttacctc
     1741 cagcaagagc agagtaaccg aaacaggcaa gaaaagctga gcgcatttgg gcttttatgc
     1801 aaaatggcgg accagaccct gttctccatt gttgagtggg ccaggagtag tatcttcttc
     1861 agggaactga aggttgatga ccaaatgaag ctgcttcaaa actgctggag tgagctcttg
     1921 attctcgatc acatttaccg acaagtggcg catgggaagg aagggacaat cttcctggtt
     1981 actggagaac acgtggacta ctccaccatc atctcacaca cagaagtcgc gttcaacaac
     2041 ctcctgagtc tcgcacagga gctggtggtg aggctccgtt cccttcagtt cgatcagcgg
     2101 gagtttgtat gtctcaagtt cctggtgctg ttcagctcag atgtgaagaa cctggagaac
     2161 ctgcagctgg tggaaggtgt ccaagagcag gtgaatgccg ccctgctgga ctacacggtt
     2221 tgcaactacc cacaacagac tgagaaattc ggacagctac ttcttcggct acccgagatc
     2281 cgggcaatca gcaagcaggc agaagactac ctgtactata agcacgtgaa cggggatgtg
     2341 ccctataata acctcctcat tgagatgctg catgccaaaa gagccctcga ggaacaaaaa
     2401 ctaatatcag aggaagattt ggaacagaaa ctaatcagtg aagaagattt agagcaaaag
     2461 ttaatttccg aggaggactt atagtgaggg cccgatatct cgacaatcaa cctctggatt
     2521 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2581 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2641 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2701 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2761 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2821 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2881 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     2941 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3001 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3061 cgagtcggat ctccctttgg gccgcctccc cgcctggaaa cgggggaggc taactgaaac
     3121 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     3181 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     3241 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     3301 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     3361 gccctgccat agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta
     3421 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3481 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3541 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc
     3601 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3661 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3721 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg
     3781 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     3841 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3901 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4081 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4141 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4201 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4261 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4321 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4381 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     4441 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4501 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4561 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4621 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4681 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4741 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     4801 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4861 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4921 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4981 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5041 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5101 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5161 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5341 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5401 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5461 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5581 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5641 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5701 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     6001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6241 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6301 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6541 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6601 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6661 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6721 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6781 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6841 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6901 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6961 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7021 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7081 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7141 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7201 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7261 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7321 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7381 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7441 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7501 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7561 tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
     7621 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     7681 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttagg
//
