LOCUS V027395 8986 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027395 VERSION V027395 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..3645 /label="Nr3c2(NM_001083906)" /note="Nr3c2(NM_001083906)" /gene="Nr3c2" CDS 3652..3678 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3682..3708 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3712..3738 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 3762..4350 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4416..4464 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4666..5094 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5108..5437 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5504..6298 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6474..6595 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6644..6660) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6668..6684 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6692..6722) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6737..6758 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7046..7634) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7805..8665) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8666..8770) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga aaccaaaggc 721 taccacagtc tccctgaagg cctagatatg gaaaggcgct ggagtcaagt gtctcagacc 781 ttggagcgtt cttctcttgg acctgcagag aggaccaatg agaacagcta catggagatt 841 gtcaacgtca gctgcgtttc cggtgctact ccgaacaaca gtactcaagg gagcagcaaa 901 gaaaaacacg aattactccc ttgtcttcag caagacaata gtcggtctgg gattttgcca 961 tcagatatta aaactgagct ggaatccaag gaactttcag ccacggtggc tgagtccatg 1021 ggtttataca tggattctgt gagagatgcc gagtacactt atgatcagca aaaccaacaa 1081 ggaagcctga gcccggcaaa gatttatcaa aacatggagc agctggtgaa gttttacaaa 1141 gagaatggtc acaggtcctc cacactgagt gctataagca ggcctttgag gtcattcatg 1201 cctgactctg ggacctccat gaatggtggg gccttgcgtg ccatcgttaa gagcccaatc 1261 atctgtcatg agaagagccc ctctgtttgc agcccgctca acatgccgtc ttcagtatgc 1321 agccccgcgg gcatcaactc catgtcctcc tccacagcta gctttggcag tttcccagtg 1381 cacagtccca tcactcaagg aacctcactg acatgctccc ccagtgttga aaatagaggc 1441 tcaaggtcac acagccccgt acatgcgagc aatgtgggct ctcctctttc aagtccatta 1501 agcagcatga aatccccaat ttccagccct ccaagtcact gcagtgtaaa atctccagta 1561 tccagtccaa acaatgtccc tctgcgctcc tctgtgtcaa gcccggcaaa tcttaacaat 1621 tcaaggtgct ctgtttccag cccttccaac accaacaata gatctacact ctccagcccg 1681 acagctagca cagtggggtc cattggcagc cccatcagca atgccttcag ctataccact 1741 tcaggcgctt cggctggagc cggtgccatc caggatatgg ttcccagtcc agacacccac 1801 gagaaaggtg ctcacgacgt tcctttccct aagacagagg aagtcgagaa ggccatttcc 1861 aatggtgtga ctggtcagct caacattgtc cagtacataa aaccagaacc agatggggct 1921 ttcagcagtt cctgcctagg aggaaacaac aaaatcaacc ccagttctcc gttctctgta 1981 ccaataaagc aagagtcaag caagcactca tgttcaggcg cctcttttaa agggaacccc 2041 acagtcaacc catttccatt catggatggc tcgtactttt cttttatgga tgataaagac 2101 tattattccc tatcaggaat cttaggacca cctgtgcccg gctttgatag tagctgtgaa 2161 ggcagtgcgt tcccgggggg gattaagcaa gaaccagatg atgggagcta tttccctgaa 2221 accagcatcc catcatctgc catcattggt gtgaattcag gtggacagtc ctttcactac 2281 cggattggtg ctcaaggtac aatatcttta tcacggtcac ctagagacca atctttccaa 2341 cacttgagtt cctttccgcc tgtcaatgca ttagtggagt catggaaacc acacggtgac 2401 ctgtcatcta ggagaagtga tgggtacccg gtcctagagt acattccaga aaacgtgtca 2461 agctctactt tacgaagtgt ttctactgga tcctcaagac cttccaagat ctgcttggtg 2521 tgtggagatg aggcttctgg gtgtcactat ggggtagtga cctgcggcag ctgcaaagtc 2581 ttcttcaaaa gagccgtgga aggacaacac aactatctgt gtgctggaag aaatgactgc 2641 attattgata agattcggag aaagaactgt cctgcctgca ggctccagaa atgcctccaa 2701 gccggcatga acttaggagc tcgaaagtca aagaagctgg ggaagttaaa aggccttcac 2761 gaggagcagc cacagcagcc cccaccgcca ccaccccaga gcccagaaga ggggaccaca 2821 tacattgctc ctaccaagga gccatcagtg aactctgcgc tggtcccgca gctcgcctcg 2881 atcacgcgtg cgctcacgcc atccccgtcc atgatcctgg agaacatcga gcccgagatc 2941 gtgtatgcag gctacgacaa ttccaagcct gacaccgccg agagcctgct ctccacgctc 3001 aaccgcctgg cgggcaagca gatgatccaa gtcgtgaagt gggccaaggt acttccagga 3061 tttaaaaact tgcctcttga ggaccaaatt accctcatcc agtattcttg gatgtgtcta 3121 tcatcgtttg ccttgagttg gagatcgtac aaacatacga acagccaatt tctctatttt 3181 gcaccagacc tagtttttaa tgaagagaag atgcatcagt ctgccatgta tgagctgtgc 3241 caggggatgc ggcagatcag ccttcagttc gtgcggcttc agctgacctt tgaggagtac 3301 tccataatga aggttttgct gctactgagc acagttccaa aagatggcct caagagccag 3361 gctgcgtttg aggagatgag gacaaattac atcaaagaac tgaggaaaat ggtcaccaag 3421 tgtcccaaca gttctggaca gagttggcag aggttctacc aactgacgaa gcttctagac 3481 tccatgcatg atttggtgaa tgacctgcta gaattctgct tctatacctt ccgagaatcc 3541 caggctctga aggtggagtt tcccgccatg ctggtggaga tcatcagcga ccagctgcca 3601 aaggtggaat ccgggaacgc caagcccctt tactttcaca ggaagctcga gtacccatac 3661 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat 3721 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca 3781 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 3841 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 3901 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 3961 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4021 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4081 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4141 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 4201 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4261 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4321 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg 4381 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 4441 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 4501 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 4561 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 4621 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg 4681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4801 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 4861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 4921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 5041 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 5101 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 5161 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 5221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 5281 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 5341 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 5401 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 5461 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 5521 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 5581 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 5641 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 5701 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 5761 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 5821 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 5881 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 5941 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 6001 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 6061 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 6121 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 6181 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 6241 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 6301 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 6361 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 6421 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 6481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 6541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6601 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 7261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 7501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 8821 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 8881 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 8941 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg //