LOCUS       V026856                 8043 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026856
VERSION     V026856
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4190
                     /label="Nlrp12(NM_001033431)"
                     /note="Nlrp12(NM_001033431)"
                     /gene="Nlrp12"
     CDS             4209..4238
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4257..4280
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4300..4316)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4327..4949
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5098..5686)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6015..6062
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6294..7088)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7123..7480)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7482..7586)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7613..8041
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gttgccgtct acagccaggg atggcctcta tcgactgtct acctacctgg
     1081 aagaactcga ggctggggaa ctgaagaaat tcaaattatt cctggggatt gcagaggacc
     1141 tgagccagga caaaattccc tggggacgaa tggagaaggc tggtcctctg gaaatggctc
     1201 agctgatggt ggcccacatg gggacaaggg aggcttggct tctggctctc agcacctttc
     1261 agaggattca caggaaggac ctgtgggagc gaggacaggg agaagacctg gtgagggtca
     1321 ctccaaataa tggtctatgc ctttttgaga gccaatcagc gtgccctttg gatgtctctc
     1381 ccaatgctcc aagaaaagat ctacagacaa cctacaaaga ctatgtccga aggaaattcc
     1441 agctaatgga agaccgcaat gcacgattag gcgaatgtgt gaacctgagc aatcgttaca
     1501 ctcggcttct cctagtaaaa gaacactcaa atcctatctg gacacagcag aaatttgtag
     1561 atgtagagtg ggaacgctcc agaaccaggc gtcaccagac tagtcctatc caaatggaga
     1621 ccctctttga gccagacgaa gaacgccccg agccaccaca cacagtggta ttacaagggg
     1681 cagcggggat ggggaagtcc atgctggccc acaaagtgat gttggactgg gccgatggga
     1741 ggctcttcca aggccggttt gattatgtct tctatatcag ctgcagggag ttgaatagaa
     1801 gccacaccca gtgcagtgta caagacctca tctccagctg ctggccggag cgtggtatat
     1861 ccctcgaaga cctcatgcag gctcctgacc gtctcctatt catcattgat ggcttcgata
     1921 aactccatcc ttctttccat gatgctcagg gtccctggtg cctctgctgg gaggagaaac
     1981 aacctactga agtcctcctc ggaagtctga ttcggaggtt gcttctgccc caggtctctc
     2041 tgctcatcac cacacgaccc tgtgcactgg agaagctgca cggcttgcta gaacacccca
     2101 ggcacgtgga gatcctgggc ttctccgagg aagctaggaa ggaatatttc tacagatatt
     2161 tccacaacac tggacaagca agccgggtgt taagcttctt gatggactat gagcccctct
     2221 ttaccatgtg ttttgttccc atggtgtcct gggtggtctg cacctgccta aagcagcagc
     2281 tggaaagtgg ggagctttta agacaaacac ctaggaccac cacagctgtt tatatgttct
     2341 accttctgag cctgatgcag cccaagccag ggactccaac cttcaaagtc ccagccaacc
     2401 agagaggcct ggtctctctg gctgcagagg gcctctggaa tcagaagatt ctatttgatg
     2461 aacaggatct tgggaaacac ggcctagatg gagcagatgt gtccactttc ctcaacgtga
     2521 acatattcca gaagggtatc aaatgtgaga aattctacag cttcatccac ctgagtttcc
     2581 aggaattctt cgcagccatg tactgtgcac tgaatggcag agaggcggtg aggagagcgc
     2641 tggctgagta tggtttttcg gaaaggaact tcttggccct cacggtccac tttctgtttg
     2701 gcctcctcaa cgaagagatg agatgctacc ttgagaggaa tctcggctgg agcatctccc
     2761 ctcaggtgaa ggaggaagtg ttggcatgga tccaaaacaa ggctgggagt gaaggctcca
     2821 ccctgcagca tggctccctg gagctactca gctgcttgta tgaggtccag gaggaggact
     2881 tcatccagca ggccctgagc cactttcaag tggttgtagt cagaagcatc tcaacaaaga
     2941 tggagcacat ggtctgctcg ttttgtgcga ggtattgcag aagtacagaa gtgcttcact
     3001 tgcatgggag tgcttatagt acaggcatgg aggacgaccc accagaacct tcaggagtcc
     3061 agactcagtc cacatactta caggaaagga acatgctgcc tgatgtctac agtgcatacc
     3121 tttcagcagc tgtctgtacc aactccaacc tgatcgagct ggccttatac cgaaatgcct
     3181 tgggcagcca gggtgtaagg ctgctctgtc aaggcctccg acatgccagc tgcaagctgc
     3241 agaacctgag gctgaagagg tgtcagatct ccggatcagc ctgccaggac ctcgcagccg
     3301 ctgtcatcgc caacaggaat ttaatcaggc tggacctcag tgacaacagc attggggtgc
     3361 caggcctgga gctgctctgt gaggggctgc agcaccccag gtgtaggctg cagatgatcc
     3421 agctgaggaa gtgtctgttg gaggctgcag ctggccgatc cctggcttct gttctcagca
     3481 acaactcata tctggtagaa ctggatctga caggaaaccc cttggaagat tcggggctga
     3541 agttactgtg tcaagggcta aggcaccctg tctgcaggct gcgtaccctg tggctgaaga
     3601 tctgccacct tggacaagct tcctgcgaag atctggcctc tactctcaaa atgaaccaga
     3661 gcctgctgga gctggacctg ggtctgaatg atcttggaga ttctggggtg cttctgctgt
     3721 gtgaaggcct cagtcatcca gattgcaaac tccagaccct tcggttgggc atttgccgac
     3781 tgggctcagt cgcgtgtgtg gggatcgcca gtgtgctcca ggtcaacaca tgcctccaag
     3841 agctggacct gagcttcaat gacttgggag acaggggcct gcagctgctg ggggaaggcc
     3901 tgaggcacca gacctgcaga ctccagaagc tgtggctgga caactgcgga ctcacctcca
     3961 aagcatgtga ggacctttct tctatcctgg gaatcagcca gaccctgcat gagctttatt
     4021 tgaccaataa tgctctgggg gacacaggtg tctgtctgct gtgcaagagg ctgaggcatc
     4081 caggctgcaa gcttcgagtc ctgtggctgt ttgggatgga cctgaataaa aagactcaca
     4141 ggaggatggc agcacttcga gtcacaaaac cgtacctgga tattgggtgt acgcgtacgc
     4201 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     4261 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     4321 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     4381 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     4441 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     4501 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     4561 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     4621 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     4681 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     4741 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     4801 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     4861 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     4921 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     4981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5821 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     5881 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     5941 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     6001 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     6061 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     6121 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     6181 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     6241 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     6301 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     6361 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     6421 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     6481 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6541 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6601 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6661 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     6721 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     6781 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     6841 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     6901 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     6961 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     7021 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     7081 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     7141 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7201 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7261 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     7321 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     7381 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     7441 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     7501 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7561 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     7621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8041 ttt
//
