LOCUS V018502 5527 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018502 VERSION V018502 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 991..1734 /label="Ndufv2(NM_028388)" /note="Ndufv2(NM_028388)" /gene="Ndufv2" CDS 1738..1767 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" primer_bind complement(1784..1800) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1811..2433 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2582..3170) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3499..3546 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3778..4572) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4607..4964) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4966..5070) /gene="bla" /label="AmpR promoter" rep_origin 5097..5525 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgccacc atgttctcct tggcgctgcg ggccagggcg 1021 accggcctcg ctgctcagtg gggaagacat gcaaggaatt tgcataagac agcagtgcac 1081 aatggtgctg gaggagcctt atttgtgcat agagatactc ctgagaataa cccagatact 1141 ccatttgatt tcacaccaga aaactataag aggatagagg caatagtaaa aaactaccca 1201 gaagggcatc aagccgctgc tgtgcttcca gtcctggatc tcgcccaaag gcagaatgga 1261 tggctaccta tctccgctat gaacaaggtg gctgaagttt tacaagtacc tccaatgaga 1321 gtatatgaag tagcaacttt ttatacaatg tataatcgaa agccagttgg gaagtaccat 1381 atccaggtct gcactactac accttgcatg ctgcgagatt ctgacagcat attggagacc 1441 cttcagagaa agcttggaat aaaggttgga gagactacac ctgacaaact tttcactctt 1501 atagaagtgg aatgtttagg ggcctgtgta aatgcaccga tggttcaaat aaatgacaac 1561 tactatgagg atctgacacc caaggatatt gaagagatta ttgatgaact caaagctgga 1621 aaagttccca aaccagggcc aaggagtggc cgcttctgtt gtgagccagc tggaggcctt 1681 acttctttga ctgaaccacc caaaggacct ggctttggtg tgcaagcagg ccttctcgag 1741 cagaaactca tctcagaaga ggatctgtaa acggccggcc gcggtcatag ctgtttcctg 1801 aacagatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt 1861 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga 1921 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt 1981 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc 2041 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc 2101 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg 2161 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat 2221 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc 2281 tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac agcataggct 2341 acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg 2401 cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat 2461 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 2521 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 2581 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 2641 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 2701 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2761 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2821 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2881 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2941 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 3001 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 3061 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 3121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 3241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 3301 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt 3361 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa 3421 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg 3481 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta 3541 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc 3601 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg 3661 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat 3721 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca 3781 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc 3841 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt 3901 agccaacgct atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc 3961 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac 4021 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag 4081 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg 4141 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt 4201 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga 4261 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt 4321 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc 4381 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg 4441 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc 4501 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc 4561 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta 4621 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg 4681 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg 4741 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat 4801 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg 4861 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac 4921 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca 4981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5041 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc 5101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 5161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 5221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 5281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 5341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 5401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 5461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 5521 gaatttt //