LOCUS       V018502                 5527 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018502
VERSION     V018502
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             991..1734
                     /label="Ndufv2(NM_028388)"
                     /note="Ndufv2(NM_028388)"
                     /gene="Ndufv2"
     CDS             1738..1767
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(1784..1800)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1811..2433
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2582..3170)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3499..3546
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3778..4572)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4607..4964)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4966..5070)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5097..5525
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgccacc atgttctcct tggcgctgcg ggccagggcg
     1021 accggcctcg ctgctcagtg gggaagacat gcaaggaatt tgcataagac agcagtgcac
     1081 aatggtgctg gaggagcctt atttgtgcat agagatactc ctgagaataa cccagatact
     1141 ccatttgatt tcacaccaga aaactataag aggatagagg caatagtaaa aaactaccca
     1201 gaagggcatc aagccgctgc tgtgcttcca gtcctggatc tcgcccaaag gcagaatgga
     1261 tggctaccta tctccgctat gaacaaggtg gctgaagttt tacaagtacc tccaatgaga
     1321 gtatatgaag tagcaacttt ttatacaatg tataatcgaa agccagttgg gaagtaccat
     1381 atccaggtct gcactactac accttgcatg ctgcgagatt ctgacagcat attggagacc
     1441 cttcagagaa agcttggaat aaaggttgga gagactacac ctgacaaact tttcactctt
     1501 atagaagtgg aatgtttagg ggcctgtgta aatgcaccga tggttcaaat aaatgacaac
     1561 tactatgagg atctgacacc caaggatatt gaagagatta ttgatgaact caaagctgga
     1621 aaagttccca aaccagggcc aaggagtggc cgcttctgtt gtgagccagc tggaggcctt
     1681 acttctttga ctgaaccacc caaaggacct ggctttggtg tgcaagcagg ccttctcgag
     1741 cagaaactca tctcagaaga ggatctgtaa acggccggcc gcggtcatag ctgtttcctg
     1801 aacagatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     1861 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     1921 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     1981 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     2041 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     2101 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     2161 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     2221 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     2281 tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac agcataggct
     2341 acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg
     2401 cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat
     2461 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2521 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2581 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2641 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2701 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2761 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2821 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2881 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2941 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3001 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3061 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3301 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt
     3361 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
     3421 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     3481 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta
     3541 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc
     3601 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     3661 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     3721 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     3781 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     3841 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     3901 agccaacgct atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc
     3961 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     4021 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag
     4081 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     4141 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     4201 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     4261 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     4321 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     4381 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     4441 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     4501 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     4561 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta
     4621 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     4681 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     4741 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     4801 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     4861 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     4921 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
     4981 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5041 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5521 gaatttt
//
