LOCUS       V034480                 9087 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034480
VERSION     V034480
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..3767
                     /label="Mov10(NM_001163440)"
                     /note="Mov10(NM_001163440)"
                     /gene="Mov10"
     CDS             3774..3839
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    3863..4451
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4517..4565
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4767..5195
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5209..5538
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5605..6399
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6575..6696
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6745..6761)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6769..6785
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6793..6823)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6838..6859
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7147..7735)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7906..8766)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8767..8871)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatgcc tagcaagttc agctgccgaa
      781 agctccggga gaccggccag aggttcgaga gttttctggc cgaacgtgga ctggacctgg
      841 agacagatcg tgagcggctg cggacgattt acaaccacga cttcaagccc agctatggga
      901 cccctgcccc tggcttctcc tccatgctgt atggaatgaa gatcgcaaat ctggccttcg
      961 tcaccaagac tcgggtcagg ttcttcaaac tagaccgctg ggctgatgtg cagttaccag
     1021 aaaagaggcg aataaagcca gggtcgaaca tcagcaaaca acacagatca ctgttggcca
     1081 ggatctttca cgacagggct gagtaccttc atgggaagca tggggtagac gtggaggtcc
     1141 aggggcccca tgaagcccga gacgggcaac tccttatcca cctggatttg aaccgcaagg
     1201 aggtactaac cctacggctt cggaacggcg gaagcaaacc tgtcaccctc actcacctat
     1261 tcccactgtg ctggacgccc cagtttgtct tctaccatgg agaacaggac ctgccctgcc
     1321 cactgggccc cggtgaaagc tatgaactcc acatctactg taagaccagc attgtgggtt
     1381 acttcccagc cactgtcctc tgggaactcc tgggacccgg ggagtcggga gcagaaggag
     1441 cagaaacttt ctacattgcc cgattcctgg cggctgtcgc ccacagtccc ctggctgccc
     1501 agttgaaacc cacaactccc ttcaaacgcc cccctcggct caccagaaac tctgtgttga
     1561 ccaaccggat cgaggaagga gagaggcccg accgagccaa gggctatgaa ctagagctaa
     1621 gtttggccct ggggacctat tacccaccca tcctcctccg acaactgctc cctacccttc
     1681 ttcagggacc aagtatcttc actgccccaa aggaggttgc tgagatcaag gcccagctgg
     1741 agacaaccct gaaatccagg aactatgagg tgaaactccg gctgctgctg cacctggaag
     1801 agctgcagat ggagcatgac atccggcact atgacctgga ctcggtaccc atgacctggg
     1861 accctgtgga ccagaatccc aggctgctca ccctggaggt tcctggtgtc gcagagagcc
     1921 gtccctcagt gctacgaggt gaccaccttt ttgccctttt gtcctctgag acccaacagg
     1981 acgaccctgt cacctacaag ggtttcgtgc acaaggtgga actggaccgt gtcaagctga
     2041 gcttttctac aagcctcctg agccgatttg tggatgggct gaccttcaag gtgaacttta
     2101 ccttcaaccg ccagcccctt cgggtccagc accgggccct agagttgacg gggcgctggg
     2161 tgctatggcc catgcttttt cctgtggcct cccgtggggt ctcgttgctg ccctcagatg
     2221 tgaagttcaa gctgtacgat cggagtctgg agtcaaaccc tgagcaactg caggccatga
     2281 agcacattgt gaggggtacc acccggcctg ccccctacat catctttggg cctccaggta
     2341 ccggcaagac tgtcacatta gtggaggcca tcaaacaggt agtgaagcat ttgcccaaag
     2401 cccacatcct ggcctgtgct ccatccaact caggggctga cctcctctgt cagcggctcc
     2461 gggtccacct gcccagctcc atctaccgtc tcctggcccc cagcagggac atccgaatgg
     2521 tgcctgagga cattaagacc tgctgtaact gggatgctaa gaagggagaa tatgtgtatc
     2581 ctgctaagaa gcacctgcag caatatcggg tcttaattac caccctcatc actgccagca
     2641 ggttggtgtc agcccagttt cccatcgatc acttcacaca catcttcatc gatgaggctg
     2701 gccactgcat ggagcctgag agtctggtgg ccatagcagg actgatggat gtcaaggaaa
     2761 cgggcaatcc cggagggcag ctggtactgg caggagaccc tcggcagctg gggcctgtgc
     2821 tgcgctcgcc actagccctg aagcatggac ttggctactc tctgctagag cgcctgctcg
     2881 cctacaactc cttgtataag aagggcccca atggctatga cccccagttc atcaccaaac
     2941 tgctccgcaa ctacaggtct caccccacca tcctggacat ccctaaccag ctgtactacg
     3001 acggagagct gcaggcctgc gcagatgtgg tggatcgaga acggttctgc cgctgggagg
     3061 ggctgcctca gcagggcttc cccatcattt tccatggtgt aatgggcaaa gatgagagag
     3121 aaggcaatag cccatccttc ttcaaccctg aagaggctgc tacagtgacg tcatacctga
     3181 aacaactcct ggccccttcc tccaagaagg gtaaagcccg cctgagcccc cgaaatgtgg
     3241 gcgtcatctc cccgtaccgg aagcaggtag aaaaaatccg ttactgcatc acaaaacttg
     3301 accgagaact tcgcggactg gatgacatca aagatttgaa ggtgggctct gtggaagagt
     3361 tccaaggcca agaacgcagc gtcatcctca tctccaccgt ccgaagcagc cagagctttg
     3421 tacagctgga tctagacttt aacctcggtt tccttaagaa ccccaagagg ttcaatgttg
     3481 ctgtgacccg agccaaggct ttgctcatcg tagtgggcaa ccccctcctc ctaggccacg
     3541 acccagactg gaaaacgttc ctggagttct gtaaagaaaa cgggggatat accgggtgcc
     3601 cctttcctgc caaactggac ctgcagcagg gacaggactt gctccaaggt ctgagcaaac
     3661 tcagcccctc tacctcaggg ccccgccgtc accagaatct cccccaggag cgggagggtg
     3721 aagggggcct gcccttacaa gtggagccag agtggagaaa tgagctcgga tccgactaca
     3781 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     3841 agtgagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     3901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4381 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4441 cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     4501 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     4561 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     4621 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     4681 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     4741 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4801 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4861 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4921 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4981 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5041 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5101 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
     5161 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5221 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5281 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5341 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5401 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5461 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5521 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5581 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5641 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5701 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5761 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5821 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5881 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5941 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6001 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6061 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6121 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6181 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6241 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6301 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6361 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     6421 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6481 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6541 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6601 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6661 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6721 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7321 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7561 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8881 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact
     8941 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9001 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9061 gacaattgca tgaagaatct gcttagg
//
