LOCUS V038997 7186 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038997 VERSION V038997 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1845 /label="Mapk3(NM_011952)" /note="Mapk3(NM_011952)" /gene="Mapk3" CDS 1852..1878 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1882..1908 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1912..1938 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 1962..2550 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2616..2664 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2866..3294 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3308..3637 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3704..4498 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4674..4795 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4844..4860) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4868..4884 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4892..4922) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4937..4958 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5246..5834) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6005..6865) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6866..6970) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggcggcggcg 721 gcggctccgg ggggcggggg cggggagccc aggggaactg ctggggtcgt cccggtggtc 781 cccggggagg tggaggtggt gaaggggcag ccattcgatg tgggcccacg ctacacgcag 841 ctgcagtaca tcggcgaggg cgcgtacggc atggtcagct cagcttatga ccacgtgcgc 901 aagaccagag tggccatcaa gaagatcagc ccctttgagc atcaaaccta ctgtcagcgc 961 acgctgaggg agatccagat cttgctgcga ttccgccatg agaatgttat aggcatccga 1021 gacatcctca gagcgcccac cctggaagcc atgagagatg tttacattgt tcaggacctc 1081 atggagacag acctgtacaa gctgcttaaa agccagcagc tgagcaatga ccacatctgc 1141 tacttcctct accagatcct ccggggcctc aagtatatac actcagccaa tgtgctgcac 1201 cgggacctga agccttccaa tctgcttatc aacaccacct gcgaccttaa gatctgtgat 1261 tttggcctgg cccggattgc tgaccctgag cacgaccaca ctggctttct gacggagtat 1321 gtggccacac gctggtaccg agccccagag atcatgctta attccaaggg ctacaccaaa 1381 tccatcgaca tctggtctgt gggctgcatt ctggctgaga tgctctccaa ccggcccatc 1441 ttccccggca agcactacct ggaccagctc aaccacattc taggtatctt gggttcccca 1501 tcccaggagg accttaattg catcattaac atgaaggccc gaaactacct gcagtctctg 1561 ccctcgaaaa ccaaggtggc ttgggccaag ctctttccta aatctgactc caaagctctt 1621 gacctgctgg accggatgtt aaccttcaac ccaaacaagc gcatcacagt agaggaagcg 1681 ctggctcacc cttacctgga acagtactac gatccgacag atgagccagt ggccgaggag 1741 ccattcacct tcgacatgga gctggatgac ctccccaagg agcggctgaa ggagttgatc 1801 ttccaggaga cagcccgctt ccagccaggg gcgccagagg gccccctcga gtacccatac 1861 gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag ctatccttat 1921 gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct ctggattaca 1981 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 2041 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 2101 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 2161 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 2221 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 2281 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 2341 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 2401 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 2461 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 2521 gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg 2581 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 2641 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 2701 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 2761 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 2821 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg 2881 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2941 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3001 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 3061 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 3121 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 3181 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 3241 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 3301 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3361 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3421 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3541 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3601 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3661 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3721 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3781 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3841 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3901 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3961 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4021 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4081 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4141 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4201 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4261 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4321 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4381 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4441 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4501 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4561 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4621 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 4681 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4741 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 4801 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 4861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 4921 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 4981 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5041 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 5461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5881 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5941 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6001 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6061 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6421 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6961 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc 7021 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 7081 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 7141 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg //