LOCUS       V031098                 6021 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031098
VERSION     V031098
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2168
                     /label="Mageb1(NM_010759)"
                     /note="Mageb1(NM_010759)"
                     /gene="Mageb1"
     CDS             2187..2216
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2235..2258
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2278..2294)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2305..2927
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3076..3664)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3993..4040
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4272..5066)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5101..5458)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5460..5564)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5591..6019
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gttctcctgg aaagcttcaa aagccaggtc tccattaagt ccaaggtatt
     1081 ctctacctgg tagtacagag gtacttacag gttgtcattc ttatccttcc agattcctgt
     1141 ctgccagctc ttttacttca gccctgagca cagtcaacat gcctaggggt caaaagagta
     1201 agacccgctc ccgtgcaaaa cgacagcagt cacgcaggga ggttccagta gttcagccca
     1261 ctgcagagga agcagggtct tctcctgttg accagagtgc tgggtccagc ttccctggtg
     1321 gttctgctcc tcagggtgtg aaaacccctg gatcttttgg tgcaggtgta tcctgcacag
     1381 gctctggtat aggtggtaga aatgctgctg tcctgcctga tacaaaaagt tcagatggca
     1441 cccaggcagg gacttccatt cagcacacac tgaaagatcc tatcatgagg aaggctagtg
     1501 tgctgataga attcctgcta gataaattta agatgaaaga agcagttaca aggagtgaaa
     1561 tgctggcagt agttaacaag aagtataagg agcaattccc tgagatcctc aggagaactt
     1621 ctgcacgcct agaattagtc tttggtcttg agttgaagga aattgatccc agcactcatt
     1681 cctatttgct ggtaggcaaa ctgggtcttt ccactgaggg aagtttgagt agtaactggg
     1741 ggttgcctag gacaggtctc ctaatgtctg tcctaggtgt gatcttcatg aagggtaacc
     1801 gtgccactga gcaagaggtc tggcaatttc tgcatggagt gggggtatat gctgggaaga
     1861 agcacttgat ctttggcgag cctgaggagt ttataagaga tgtagtgcgg gaaaattacc
     1921 tggagtaccg ccaggtacct ggcagtgatc ccccaagcta tgagttcctg tggggaccca
     1981 gagcccatgc tgaaacaacc aagatgaaag tcctggaagt tttagctaaa gtcaatggca
     2041 cagtccctag tgccttccct aatctctacc agttggctct tagagatcag gcaggagggg
     2101 tgccaagaag gagagttcaa ggcaagggtg ttcattccaa ggccccatcc caaaagtcct
     2161 ctaacatgac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
     2221 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
     2281 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     2341 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     2401 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     2461 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     2521 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     2581 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     2641 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     2701 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     2761 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     2821 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     2881 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     3841 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     3901 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     3961 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     4021 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     4081 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     4141 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     4201 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     4261 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     4321 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     4381 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     4441 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     4501 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     4561 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     4621 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     4681 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     4741 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     4801 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     4861 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     4921 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     4981 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     5041 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     5101 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     5161 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     5221 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     5281 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5341 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     5401 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     5461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5581 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6001 caaaaattta acgcgaattt t
//
