LOCUS V031098 6021 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031098 VERSION V031098 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2168 /label="Mageb1(NM_010759)" /note="Mageb1(NM_010759)" /gene="Mageb1" CDS 2187..2216 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2235..2258 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2278..2294) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2305..2927 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3076..3664) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3993..4040 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4272..5066) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5101..5458) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5460..5564) /gene="bla" /label="AmpR promoter" rep_origin 5591..6019 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gttctcctgg aaagcttcaa aagccaggtc tccattaagt ccaaggtatt 1081 ctctacctgg tagtacagag gtacttacag gttgtcattc ttatccttcc agattcctgt 1141 ctgccagctc ttttacttca gccctgagca cagtcaacat gcctaggggt caaaagagta 1201 agacccgctc ccgtgcaaaa cgacagcagt cacgcaggga ggttccagta gttcagccca 1261 ctgcagagga agcagggtct tctcctgttg accagagtgc tgggtccagc ttccctggtg 1321 gttctgctcc tcagggtgtg aaaacccctg gatcttttgg tgcaggtgta tcctgcacag 1381 gctctggtat aggtggtaga aatgctgctg tcctgcctga tacaaaaagt tcagatggca 1441 cccaggcagg gacttccatt cagcacacac tgaaagatcc tatcatgagg aaggctagtg 1501 tgctgataga attcctgcta gataaattta agatgaaaga agcagttaca aggagtgaaa 1561 tgctggcagt agttaacaag aagtataagg agcaattccc tgagatcctc aggagaactt 1621 ctgcacgcct agaattagtc tttggtcttg agttgaagga aattgatccc agcactcatt 1681 cctatttgct ggtaggcaaa ctgggtcttt ccactgaggg aagtttgagt agtaactggg 1741 ggttgcctag gacaggtctc ctaatgtctg tcctaggtgt gatcttcatg aagggtaacc 1801 gtgccactga gcaagaggtc tggcaatttc tgcatggagt gggggtatat gctgggaaga 1861 agcacttgat ctttggcgag cctgaggagt ttataagaga tgtagtgcgg gaaaattacc 1921 tggagtaccg ccaggtacct ggcagtgatc ccccaagcta tgagttcctg tggggaccca 1981 gagcccatgc tgaaacaacc aagatgaaag tcctggaagt tttagctaaa gtcaatggca 2041 cagtccctag tgccttccct aatctctacc agttggctct tagagatcag gcaggagggg 2101 tgccaagaag gagagttcaa ggcaagggtg ttcattccaa ggccccatcc caaaagtcct 2161 ctaacatgac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag 2221 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc 2281 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc 2341 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc 2401 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg 2461 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc 2521 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt 2581 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa 2641 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc 2701 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc 2761 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag 2821 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc 2881 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca 2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc 3841 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg 3901 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga 3961 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt 4021 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt 4081 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag 4141 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc 4201 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc 4261 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 4321 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 4381 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca 4441 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 4501 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt 4561 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 4621 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 4681 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 4741 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 4801 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 4861 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg 4921 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 4981 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 5041 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc 5101 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc 5161 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg 5221 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc 5281 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 5341 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 5401 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga 5461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 5521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 5581 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 5641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 5701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 5761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 5821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 5881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 5941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 6001 caaaaattta acgcgaattt t //