LOCUS       V038637                 8823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038637
VERSION     V038637
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             720..1373
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1410..3533
                     /label="MRE11(NM_005591)"
                     /note="MRE11(NM_005591)"
                     /gene="MRE11"
     misc_feature    3599..4187
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4253..4301
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4503..4931
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4945..5274
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5341..6135
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6311..6432
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6481..6497)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6505..6521
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6529..6559)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6574..6595
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6883..7471)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7642..8502)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8503..8607)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagccacca
      721 tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt
      781 tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat
      841 ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg
      901 gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca
      961 tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg
     1021 atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg
     1081 attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa
     1141 catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg
     1201 ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa
     1261 aacgtattga agctatccca caaattgata agtacttgaa atccagcaag tatatagcat
     1321 ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaaggaggag
     1381 ggggaagcgg cggcggaggg agtaagctta tgagtactgc agatgcactt gatgatgaaa
     1441 acacatttaa aatattagtt gcaacagata ttcatcttgg atttatggag aaagatgcag
     1501 tcagaggaaa tgatacgttt gtaacactcg atgaaatttt aagacttgcc caggaaaatg
     1561 aagtggattt tattttgtta ggtggtgatc tttttcatga aaataagccc tcaaggaaaa
     1621 cattacatac ctgcctcgag ttattaagaa aatattgtat gggtgatcgg cctgtccagt
     1681 ttgaaattct cagtgatcag tcagtcaact ttggttttag taagtttcca tgggtgaact
     1741 atcaagatgg caacctcaac atttcaattc cagtgtttag tattcatggc aatcatgacg
     1801 atcccacagg ggcagatgca ctttgtgcct tggacatttt aagttgtgct ggatttgtaa
     1861 atcactttgg acgttcaatg tctgtggaga agatagacat tagtccggtt ttgcttcaaa
     1921 aaggaagcac aaagattgcg ctatatggtt taggatccat tccagatgaa aggctctatc
     1981 gaatgtttgt caataaaaaa gtaacaatgt tgagaccaaa ggaagatgag aactcttggt
     2041 ttaacttatt tgtgattcat cagaacagga gtaaacatgg aagtactaac ttcattccag
     2101 aacaattttt ggatgacttc attgatcttg ttatctgggg ccatgaacat gagtgtaaaa
     2161 tagctccaac caaaaatgaa caacagctgt tttatatctc acaacctgga agctcagtgg
     2221 ttacttctct ttccccagga gaagctgtaa agaaacatgt tggtttgctg cgtattaaag
     2281 ggaggaagat gaatatgcat aaaattcctc ttcacacagt gcggcagttt ttcatggagg
     2341 atattgttct agctaatcat ccagacattt ttaacccaga taatcctaaa gtaacccaag
     2401 ccatacaaag cttctgtttg gagaagattg aagaaatgct tgaaaatgct gaacgggaac
     2461 gtctgggtaa ttctcaccag ccagagaagc ctcttgtacg actgcgagtg gactatagtg
     2521 gaggttttga acctttcagt gttcttcgct ttagccagaa atttgtggat cgggtagcta
     2581 atccaaaaga cattatccat tttttcaggc atagagaaca aaaggaaaaa acaggagaag
     2641 agatcaactt tgggaaactt atcacaaagc cttcagaagg aacaacttta agggtagaag
     2701 atcttgtaaa acagtacttt caaaccgcag agaagaatgt gcagctctca ctgctaacag
     2761 aaagagggat gggtgaagca gtacaagaat ttgtggacaa ggaggagaaa gatgccattg
     2821 aggaattagt gaaataccag ttggaaaaaa cacagcgatt tcttaaagaa cgtcatattg
     2881 atgccctcga agacaaaatc gatgaggagg tacgtcgttt cagagaaacc agacaaaaaa
     2941 atactaatga agaagatgat gaagtccgtg aggctatgac cagggccaga gcactcagat
     3001 ctcagtcaga ggagtctgct tctgccttta gtgctgatga ccttatgagt atagatttag
     3061 cagaacagat ggctaatgac tctgatgata gcatctcagc agcaaccaac aaaggaagag
     3121 gccgaggaag aggtcgaaga ggtggaagag ggcagaattc agcatcgaga ggagggtctc
     3181 aaagaggaag agcagacact ggtctggaga cttctacccg tagcaggaac tcaaagactg
     3241 ctgtgtcagc atctagaaat atgtctatta tagatgcctt taaatctaca agacagcagc
     3301 cttcccgaaa tgtcactact aagaattatt cagaggtgat tgaggtagat gaatcagatg
     3361 tggaagaaga catttttcct accacttcaa agacagatca aaggtggtcc agcacatcat
     3421 ccagcaaaat catgtcccag agtcaagtat cgaaaggggt tgattttgaa tcaagtgagg
     3481 atgatgatga tgatcctttt atgaacacta gttctttaag aagaaataga agataagaat
     3541 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgat atctcgacaa
     3601 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3661 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3721 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3781 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3841 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3901 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3961 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     4021 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4081 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4141 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg
     4201 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4261 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4321 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4381 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4441 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
     4501 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     4681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4861 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4921 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4981 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5041 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5101 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5161 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5221 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5281 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     5341 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5401 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5461 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5521 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5581 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5641 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5701 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5761 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5821 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5881 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5941 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6001 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6061 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6121 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
     6181 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6241 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6301 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6361 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6421 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     6481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7321 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     8641 cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc
     8701 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8761 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8821 agg
//
