LOCUS       V020623                 7646 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020623
VERSION     V020623
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..2302
                     /label="MAVS(NM_020746)"
                     /note="MAVS(NM_020746)"
                     /gene="MAVS"
     CDS             2309..2398
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2422..3010
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3076..3124
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3326..3754
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3768..4097
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4164..4958
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5134..5255
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5304..5320)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5328..5344
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5352..5382)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5397..5418
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5706..6294)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6465..7325)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7326..7430)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgccgtt tgctgaagac aagacctata agtatatctg
      721 ccgcaatttc agcaattttt gcaatgtgga tgttgtagag attctgcctt acctgccctg
      781 cctcacagca agagaccagg atcgactgcg ggccacctgc acactctcag ggaaccggga
      841 caccctctgg catctcttca atacccttca gcggcggccc ggctgggtgg agtacttcat
      901 tgcggcactg aggggctgtg agctagttga tctcgcggac gaagtggcct ctgtctacca
      961 gagctaccag cctcggacct cggaccgtcc cccagaccca ctggagccac cgtcacttcc
     1021 tgctgagagg ccagggcccc ccacacctgc tgcggcccac agcatcccct acaacagctg
     1081 cagagagaag gagccaagtt accccatgcc tgtccaggag acccaggcgc cagagtcccc
     1141 aggagagaat tcagagcaag ccctgcagac gctcagcccc agagccatcc caaggaatcc
     1201 agatggtggc cccctggagt cctcctctga cctggcagcc ctcagccctc tgacctccag
     1261 cgggcatcag gagcaggaca cagaactggg cagtacccac acagcaggtg cgacctccag
     1321 cctcacacca tcccgtgggc ctgtgtctcc atctgtctcc ttccagcccc tggcccgttc
     1381 cacccccagg gcaagccgct tgcctggacc cacagggtca gttgtatcta ctggcacctc
     1441 cttctcctcc tcatcccctg gcttggcctc tgcaggggct gcagagggta aacagggtgc
     1501 agagagtgac caggccgagc ctatcatctg ctccagtggg gcagaggcac ctgccaactc
     1561 tctgccctcc aaagtgccta ccaccttgat gcctgtgaac acagtggccc tgaaagtgcc
     1621 tgccaaccca gcatctgtca gcacagtgcc ctccaagttg ccaactagct caaagccccc
     1681 tggtgcagtg ccttctaatg cgctcaccaa tccagcacca tccaaattgc ccatcaactc
     1741 aacccgtgct ggcatggtgc catccaaagt gcctactagc atggtgctca ccaaggtgtc
     1801 tgccagcaca gtccccactg acgggagcag cagaaatgag gagaccccag cagctccaac
     1861 acccgccggc gccactggag gcagctcagc ctggctagac agcagctctg agaatagggg
     1921 ccttgggtcg gagctgagta agcctggcgt gctggcatcc caggtagaca gcccgttctc
     1981 gggctgcttc gaggatcttg ccatcagtgc cagcacctcc ttgggcatgg ggccctgcca
     2041 tggcccagag gagaatgagt ataagtccga gggcaccttt gggatccacg tggctgagaa
     2101 ccccagcatc cagctcctgg agggcaaccc tgggccacct gcggacccgg atggcggccc
     2161 caggccacaa gccgaccgga agttccagga gagggaggtg ccatgccaca ggccctcacc
     2221 tggggctctg tggctccagg tggctgtgac aggggtgctg gtagtcacac tcctggtggt
     2281 gctgtaccgg cggcgtctgc acctcgagga acaaaaacta atatcagagg aagatttgga
     2341 acagaaacta atcagtgaag aagatttaga gcaaaagtta atttccgagg aggacttata
     2401 gtgagggccc gatatctcga caatcaacct ctggattaca aaatttgtga aagattgact
     2461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     2821 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2941 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3001 gcctccccgc ctggaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     3061 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     3121 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     3181 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     3241 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc
     3301 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3361 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3421 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3481 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3661 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     3721 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4021 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4081 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4141 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4201 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4261 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4321 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4381 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4441 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4501 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4561 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4621 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4681 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4741 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4801 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4861 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4921 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa
     4981 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5041 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5101 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7441 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc
     7501 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     7561 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     7621 acaattgcat gaagaatctg cttagg
//
