LOCUS       V042191                 8158 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042191
VERSION     V042191
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2817
                     /label="MARCHF7(NM_022826)"
                     /note="MARCHF7(NM_022826)"
                     /gene="MARCHF7"
     CDS             2824..2850
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2854..2880
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2884..2910
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2934..3522
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3588..3636
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3838..4266
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4280..4609
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4676..5470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5646..5767
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5816..5832)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5840..5856
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5864..5894)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5909..5930
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6218..6806)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6977..7837)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7838..7942)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga gtctaaacct
      721 tcaaggattc caagaagaat ttctgttcaa ccttccagct ccttaagtgc taggatgatg
      781 tctggaagca gaggaagtag tttaaatgat acctatcact caagagactc ttcatttaga
      841 ttggattctg aatatcagtc tacatcagca tcagcatctg cgtcaccatt tcaatctgca
      901 tggtatagtg aatctgagat aactcaggga gcacgctcaa gatcgcagaa ccagcaacgg
      961 gatcatgatt caaaaagacc taaactttcc tgtacaaact gtactacctc agctgggaga
     1021 aatgttggaa atggtttaaa cacattatca gattcatctt ggaggcatag tcaagttcct
     1081 agatcttcat caatggtact tggatcattt ggaacagact taatgagaga gaggagagat
     1141 ttggagagaa gaacagattc ctctattagt aatcttatgg attatagtca ccgaagtggt
     1201 gatttcacaa cttcatcata tgttcaagac agagttcctt catattcaca aggagcaaga
     1261 ccaaaagaaa actcaatgag cactttacag ttgaatacat catccacaaa ccaccaattg
     1321 ccttctgaac atcagaccat actaagttct agggactcca gaaattcttt aagatcaaat
     1381 ttttcttcaa gagaatcaga atcttcccga agcaatacgc agcctggatt ttcttacagt
     1441 tcaagtagag atgaagcccc aatcataagc aattcagaaa gggttgtttc atctcaaaga
     1501 ccatttcaag aatcttctga caatgaaggt aggcggacaa cgaggagatt gctgtcacgc
     1561 atagcttcta gcatgtcatc tacttttttt tcacgaagat ctagtcagga ttccttgaat
     1621 acaagatcat tgaattctga aaattcttac gtttctccaa gaatcttgac agcttcacag
     1681 tcccgtagta atgtaccatc agcttctgaa gttcccgata atagggcatc tgaagcttct
     1741 cagggatttc gatttcttag gcgaagatgg ggtttgtcat ctcttagcca caatcatagc
     1801 tctgagtcag attcagaaaa ttttaaccaa gaatctgaag gtagaaatac aggaccatgg
     1861 ttatcttcct cacttagaaa tagatgcaca cctttgttct ctagaaggag gcgagaggga
     1921 agagatgaat cttcaaggat acctacctct gatacatcat ctagatctca tatttttaga
     1981 agagaatcaa atgaagtggt tcaccttgaa gcacagaatg atcctcttgg agctgctgcc
     2041 aacagaccac aagcatctgc agcatcaagc agtgccacaa caggtggctc tacatcagat
     2101 tcggctcaag gtggaagaaa tacaggaata tcagggattc ttcctggttc cttattccgg
     2161 tttgcagtcc ctccagcact tgggagtaat ttgaccgaca atgtcatgat cacagtagat
     2221 attattcctt caggttggaa ttcagctgat ggtaaaagtg ataaaactaa aagtgcgcct
     2281 tcaagagatc cagaaagatt gcagaaaata aaagagagcc tccttttaga ggactcagaa
     2341 gaagaagaag gtgacttatg tagaatttgt caaatggcag ctgcatcatc atctaatttg
     2401 ctgatagagc catgcaagtg cacaggaagt ttgcagtatg tccaccaaga ctgtatgaaa
     2461 aagtggttac aggccaaaat taactctggt tcttcattag aagctgtaac cacctgtgaa
     2521 ctatgtaaag agaagttgga gcttaacctg gaggattttg atattcatga actacataga
     2581 gctcatgcaa atgaacaagc tgagtatgag tttatcagct ctggtctcta cctagtggtg
     2641 ttattgcact tgtgcgaaca aagcttttct gatatgatgg gaaatacaaa tgaaccaagc
     2701 acacgtgtcc gatttattaa ccttgcaaga actcttcagg cacatatgga agatctcgaa
     2761 acttcagagg atgattccga agaagacgga gaccataaca ggacatttga tattgccctc
     2821 gagtacccat acgacgtacc tgattatgca acttatcctt atgatgtacc tgattatgct
     2881 agctatcctt atgatgtccc agactacgca tagtgagggc ccgatatctc gacaatcaac
     2941 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3001 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3061 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3121 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3181 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3241 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3301 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3361 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3421 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3481 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct
     3541 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     3601 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     3661 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     3721 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     3781 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     3841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3961 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4021 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4081 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4141 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4201 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4261 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     4561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4621 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     4681 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4741 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4801 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4861 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4921 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4981 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5041 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5101 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5161 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     5221 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5281 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5341 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5401 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5461 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     5521 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5581 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5641 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5701 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5761 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5821 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5881 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5941 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6001 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7981 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata
     8041 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     8101 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
