LOCUS       V031134                 5646 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031134
VERSION     V031134
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1793
                     /label="Lrrc3c(NM_001195544)"
                     /note="Lrrc3c(NM_001195544)"
                     /gene="Lrrc3c"
     CDS             1812..1841
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1860..1883
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1903..1919)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1930..2552
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2701..3289)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3618..3665
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3897..4691)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4726..5083)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5085..5189)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5216..5644
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcccctacta gctcctcgcc tcctgcccct cctgctggtg attggcaccg
     1081 ggggctctgt gccccgcccc ctggcactgc cccagggctg ctacgtagca gaagaagccg
     1141 gggaacagac attccgctgc agccgggctg gtctgagtgc cgtgcccaac ggtatcccca
     1201 acgacacccg aaagctttac ctggacgcca accagttggc atcagtgcca gctggtgcct
     1261 tccagcacct gcctgccctg gaagaactag acctgtctca taacgctctt gttcatctct
     1321 caggggctgc tttccagggc ctggagggca ctctgcgcca cctcgacctc tctgccaacc
     1381 agctagcatc tgtgcctgtg gcagccttcg tggggctgca gatccaagtg aacctgtctg
     1441 ccaacccatg gcgctgcgac tgtgccctac aggaagtgct caggcacgtc aggttggctc
     1501 caggctccgg gacaggcatt gtgtgtggtc cagaagcccg accagatctc gtgggacacg
     1561 agttccttct gctcaccagg gaggaagagc tgtgtgggac aggacgaggt gggacccgaa
     1621 ggagcacgga tgtggccctg ttagtcacca tggggggctg gctgacactg gtagtggctt
     1681 atctgatccg ctatgtgtgg cagaaccgag atgagacgcg gcgcccggtc aagcgggctc
     1741 ctcccgcgca gcctgtgcgc tcagaggact cctctacact cagcacagtg ctcacgcgta
     1801 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg
     1861 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga
     1921 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     1981 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     2041 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     2101 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     2161 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     2221 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     2281 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     2341 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     2401 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     2461 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     2521 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc
     2581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3421 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     3481 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     3541 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     3601 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     3661 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     3721 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     3781 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     3841 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     3901 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     3961 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4021 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca
     4081 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4141 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     4201 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4261 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4321 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4381 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4441 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4501 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4561 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4621 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4681 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     4741 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     4801 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     4861 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     4921 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     4981 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5041 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa
     5101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5581 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5641 aatttt
//
