LOCUS V031138 6027 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031138 VERSION V031138 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..2174 /label="Lmx1a(NM_001417483)" /note="Lmx1a(NM_001417483)" /gene="Lmx1a" CDS 2193..2222 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2241..2264 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(2284..2300) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 2311..2933 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(3082..3670) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3999..4046 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(4278..5072) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(5107..5464) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(5466..5570) /gene="bla" /label="AmpR promoter" rep_origin 5597..6025 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gttggacggc ctgaagatgg aggagaactt tcaaagtgcg attgagacct 1081 cggcatcttt ctcctctttg ctgggcagag cggtgagccc caagtctgtc tgcgagggct 1141 gtcagcgggt catctcggac aggtttctgc tgcggctcaa cgacagcttc tggcacgagc 1201 aatgcgtgca gtgtgcctcc tgcaaagagc ccctggagac cacctgcttc taccgggaca 1261 agaagctcta ctgcaagtac cactacgaga aactgtttgc tgtcaaatgt gggggctgct 1321 tcgaggccat tgcgcccaat gagtttgtca tgcgtgccca gaagagcgta taccacctga 1381 gctgcttctg ctgctgcgtc tgtgagcgac agctgcagaa gggtgacgag tttgtcctga 1441 aggagggcca gctgctctgc aaaggggact atgagaaaga acgggagctg ctgagcctgg 1501 tgagccctgc ggcctcagac tcaggcaaaa gcgatgatga ggagagcctt tgcaagtcag 1561 cccatggggc aggaaaagga gcatcagagg acggcaagga ccataagcga cccaaacgtc 1621 ccagaaccat cctgaccact cagcagagga gagcattcaa ggcctcgttt gaagtatcct 1681 ccaagccctg cagaaaggtg agggagactc tggctgcgga gacagggctg agtgtccgtg 1741 tggttcaggt gtggttccag aaccagcgag ccaagatgaa gaagctggcc cggcgacagc 1801 agcaacagca acaggaccaa cagaacaccc agaggctgac ttctgctcag acaaatggta 1861 gtgggaatgc gggcatggaa gggatcatga acccctatac aacgttgccc accccacagc 1921 agctgctggc cattgaacag agcgtctaca actctgatcc cttccgacag ggtctcaccc 1981 caccccagat gcctggagat cacatgcacc cctatggtgc tgaacctctt ttccatgact 2041 tggatagtga tgacacatct ctcagtaacc tgggagactg cttcctggca acctcagaag 2101 ctgggcccct gcagtccaga gtgggaaacc ccattgacca tctgtactcc atgcagaatt 2161 cctatttcac ctctacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 2221 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 2281 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 2341 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2401 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2461 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2521 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2581 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 2641 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2701 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 2761 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 2821 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 2881 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 2941 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 3841 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 3901 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 3961 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 4021 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 4081 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 4141 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 4201 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 4261 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 4321 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 4381 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 4441 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 4501 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 4561 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 4621 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 4681 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 4741 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 4801 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 4861 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 4921 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 4981 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 5041 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 5101 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 5161 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 5221 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 5281 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 5341 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 5401 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 5461 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5521 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5581 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 5641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 5821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 5881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 5941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 6001 atttaacaaa aatttaacgc gaatttt //