LOCUS V029804 8268 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029804 VERSION V029804 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..4415 /label="Lats1(NM_010690)" /note="Lats1(NM_010690)" /gene="Lats1" CDS 4434..4463 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 4482..4505 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(4525..4541) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 4552..5174 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(5323..5911) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 6240..6287 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(6519..7313) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(7348..7705) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7707..7811) /gene="bla" /label="AmpR promoter" rep_origin 7838..8266 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaagaggggt gaaaagccag aagggtacag acagatgagg cctaagacct 1081 ttcctgccag caactaccct ggcagcagcc gacagatgct gcaggagatc cgagaatccc 1141 tgaggaattt atcaaaacca tctgatgctt cgaaggccga gcataacctg aataagatgt 1201 cgactgaaga tcccaggcag gtgagaaatc cacccaaatt tggcacacat cataaagcct 1261 tgcaagaaat tcgaaactct ctactaccat ttgcaaatga aacaagttct tcccggagcc 1321 cttcagaagt taatccacag atgtttcagg atttgcaggc tgctggcttt gatgaggaca 1381 tggttattca agctcttcag aaaaccaata acagaagcat agaagccgct gttgaattca 1441 ttagtaaaat gagttaccaa gaccctcgtc gagagcagat gtctgcagca gctgccaggc 1501 ctattaatgc caccatgaaa ccaggaaatg tgcaacattc aattaaccga aaacaaagct 1561 ggaaaggttc taaagagtct ctagttcctc agagacacgg cccatctcta ggagaaaatg 1621 tggtttatcg ttctgaaagc cccaactcac aggcggatgt aggaagacct ctgtctggat 1681 ccggcattgc agcatttgct caagctcacc caagcaatgg acagagagtg aaccccccac 1741 caccacctca agttaggagt gttactcctc caccacctcc gagaggccag accccacctc 1801 cccgaggcac cactccccct cccccctcat gggaaccaag ctctcagaca aagcgctact 1861 ctgggaacat ggagtacgta atctcccgaa tctcccctgt tccacctggg gcgtggcagg 1921 agggctaccc tccaccacct cttaccactt ctcccatgaa tccccctagc caggctcaga 1981 gggccattag ttctgttcca gttggtagac aacccatcat catgcagagt actagcaaat 2041 ttaactttac accagggcga cctggagttc agaatggtgg tggtcagtct gattttatcg 2101 tgcaccaaaa tgtccccact ggttctgtga ctcggcagcc accacctcca tatcctctga 2161 ccccagctaa tggacaaagc ccctctgctt tacaaacagg ggcttctgct gctccaccat 2221 cattcgccaa tggaaacgtt cctcagtcga tgatggtgcc caacaggaac agtcataaca 2281 tggagcttta taatattaat gtccctggac tgcaaacagc ctggccccag tcgtcttctg 2341 ctcctgcgca gtcatcccca agcggtgggc atgaaattcc tacatggcaa cctaacatac 2401 cagtgaggtc aaattctttt aataacccat taggaagtag agcaagtcac tctgctaatt 2461 ctcagccttc tgccactaca gtcactgcca tcacacccgc tcctattcaa cagcccgtga 2521 aaagcatgcg cgtcctgaaa ccagagctgc agactgcttt agccccaacc catccttctt 2581 ggatgccaca gccagttcag actgttcagc ctaccccttt ttctgagggt acagcttcaa 2641 gtgtgcctgt catcccacct gttgctgaag ctccaagcta tcaaggtcca ccaccgcctt 2701 atccaaaaca tctgctacac caaaacccat ctgtccctcc atatgagtca gtaagtaagc 2761 cctgcaaaga tgaacagcct agcttaccca aggaagatga tagtgagaag agtgcggaca 2821 gtggtgactc tggggataaa gaaaagaaac agattacaac ttcacctatc actgttcgga 2881 aaaacaagaa agatgaagaa cgaagagagt ctcggattca gagttactcc ccacaggcct 2941 ttaagttctt catggagcag cacgtagaga acgtcctgaa gtctcatcag cagcgtctgc 3001 atcggaagaa gcagctagaa aatgaaatga tgcgggttgg attatctcaa gatgcccagg 3061 atcaaatgag aaagatgctt tgccagaaag agtctaacta tattcgtctt aaaagggcta 3121 aaatggacaa gtctatgttt gtaaagataa agacattagg aataggagcg tttggtgaag 3181 tctgtctagc aagaaaagtc gatactaaag ctttgtatgc aacaaagact cttcgaaaga 3241 aagacgttct gctccgaaat caggtggctc atgtgaaagc ggagagggat atcctagcag 3301 aagccgacaa tgagtgggtg gtccgcctgt actactcttt ccaggacaag gacaacttgt 3361 actttgtgat ggactacatt cctggggggg atatgatgag cctattaatt agaatgggca 3421 tctttcctga aaatctggca cgattctaca tagcagaact tacctgtgca gttgaaagtg 3481 ttcataaaat gggttttatt catagagata ttaaacctga taacattttg attgaccgtg 3541 atggccatat taaattgact gactttggct tgtgcactgg cttcagatgg acacatgact 3601 ccaagtacta ccagagtggg gatcacccac ggcaagatag catggatttc agtaacgaat 3661 ggggagatcc ttccaattgt cggtgtgggg acagactgaa gccactggag cggagagctg 3721 ctcgccagca ccagcgatgt ctagcccatt ctctggttgg gactcccaat tatattgcac 3781 ctgaagtgct actgcgaaca ggatatacac agctgtgtga ctggtggagt gttggtgtta 3841 ttctttttga aatgttggtg ggacaacctc ctttcttggc acaaacccca ttagaaacac 3901 aaatgaaggt tatcaactgg caaacttctc ttcacatccc tcctcaagct aagctgagtc 3961 ctgaagcctc tgacctcatt atcaaacttt gtcgaggacc agaagaccgc ctcggcaaga 4021 acggtgctga tgagataaag gctcatccat tttttaagac catcgatttc tctagtgatc 4081 tgagacagca gtctgcttca tacatcccta aaatcacgca tccaacagat acatccaatt 4141 tcgaccctgt tgatcctgat aaattgtgga gcgatggcag cgaggaggaa aatatcagtg 4201 acactctgaa cggatggtat aaaaatggga agcaccccga gcacgctttc tatgagttca 4261 cctttcggag gttttttgat gacaatggct acccatataa ttatccaaag cctattgagt 4321 atgaatacat tcattcacag ggctcagaac aacagtctga tgaagatgat caacacacaa 4381 gctccgatgg aaacaaccga gatctagtgt atgttacgcg tacgcggccg ctcgagcaga 4441 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg 4501 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat 4561 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc 4621 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat 4681 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg 4741 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca 4801 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc 4861 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc 4921 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc 4981 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa 5041 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg 5101 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag 5161 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt 5221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 5941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6061 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc 6121 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca 6181 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg 6241 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt 6301 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa 6361 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc 6421 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg 6481 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg 6541 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg 6601 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga 6661 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc 6721 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc 6781 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc 6841 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt 6901 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca 6961 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc 7021 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct 7081 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca 7141 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc 7201 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc 7261 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac 7321 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc 7381 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa 7441 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg 7501 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct 7561 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct 7621 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt 7681 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat 7741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 7801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 7861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 7921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 7981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 8041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 8101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 8161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 8221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt //