LOCUS       V029804                 8268 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029804
VERSION     V029804
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..4415
                     /label="Lats1(NM_010690)"
                     /note="Lats1(NM_010690)"
                     /gene="Lats1"
     CDS             4434..4463
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             4482..4505
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4525..4541)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4552..5174
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(5323..5911)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    6240..6287
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(6519..7313)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(7348..7705)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(7707..7811)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7838..8266
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaagaggggt gaaaagccag aagggtacag acagatgagg cctaagacct
     1081 ttcctgccag caactaccct ggcagcagcc gacagatgct gcaggagatc cgagaatccc
     1141 tgaggaattt atcaaaacca tctgatgctt cgaaggccga gcataacctg aataagatgt
     1201 cgactgaaga tcccaggcag gtgagaaatc cacccaaatt tggcacacat cataaagcct
     1261 tgcaagaaat tcgaaactct ctactaccat ttgcaaatga aacaagttct tcccggagcc
     1321 cttcagaagt taatccacag atgtttcagg atttgcaggc tgctggcttt gatgaggaca
     1381 tggttattca agctcttcag aaaaccaata acagaagcat agaagccgct gttgaattca
     1441 ttagtaaaat gagttaccaa gaccctcgtc gagagcagat gtctgcagca gctgccaggc
     1501 ctattaatgc caccatgaaa ccaggaaatg tgcaacattc aattaaccga aaacaaagct
     1561 ggaaaggttc taaagagtct ctagttcctc agagacacgg cccatctcta ggagaaaatg
     1621 tggtttatcg ttctgaaagc cccaactcac aggcggatgt aggaagacct ctgtctggat
     1681 ccggcattgc agcatttgct caagctcacc caagcaatgg acagagagtg aaccccccac
     1741 caccacctca agttaggagt gttactcctc caccacctcc gagaggccag accccacctc
     1801 cccgaggcac cactccccct cccccctcat gggaaccaag ctctcagaca aagcgctact
     1861 ctgggaacat ggagtacgta atctcccgaa tctcccctgt tccacctggg gcgtggcagg
     1921 agggctaccc tccaccacct cttaccactt ctcccatgaa tccccctagc caggctcaga
     1981 gggccattag ttctgttcca gttggtagac aacccatcat catgcagagt actagcaaat
     2041 ttaactttac accagggcga cctggagttc agaatggtgg tggtcagtct gattttatcg
     2101 tgcaccaaaa tgtccccact ggttctgtga ctcggcagcc accacctcca tatcctctga
     2161 ccccagctaa tggacaaagc ccctctgctt tacaaacagg ggcttctgct gctccaccat
     2221 cattcgccaa tggaaacgtt cctcagtcga tgatggtgcc caacaggaac agtcataaca
     2281 tggagcttta taatattaat gtccctggac tgcaaacagc ctggccccag tcgtcttctg
     2341 ctcctgcgca gtcatcccca agcggtgggc atgaaattcc tacatggcaa cctaacatac
     2401 cagtgaggtc aaattctttt aataacccat taggaagtag agcaagtcac tctgctaatt
     2461 ctcagccttc tgccactaca gtcactgcca tcacacccgc tcctattcaa cagcccgtga
     2521 aaagcatgcg cgtcctgaaa ccagagctgc agactgcttt agccccaacc catccttctt
     2581 ggatgccaca gccagttcag actgttcagc ctaccccttt ttctgagggt acagcttcaa
     2641 gtgtgcctgt catcccacct gttgctgaag ctccaagcta tcaaggtcca ccaccgcctt
     2701 atccaaaaca tctgctacac caaaacccat ctgtccctcc atatgagtca gtaagtaagc
     2761 cctgcaaaga tgaacagcct agcttaccca aggaagatga tagtgagaag agtgcggaca
     2821 gtggtgactc tggggataaa gaaaagaaac agattacaac ttcacctatc actgttcgga
     2881 aaaacaagaa agatgaagaa cgaagagagt ctcggattca gagttactcc ccacaggcct
     2941 ttaagttctt catggagcag cacgtagaga acgtcctgaa gtctcatcag cagcgtctgc
     3001 atcggaagaa gcagctagaa aatgaaatga tgcgggttgg attatctcaa gatgcccagg
     3061 atcaaatgag aaagatgctt tgccagaaag agtctaacta tattcgtctt aaaagggcta
     3121 aaatggacaa gtctatgttt gtaaagataa agacattagg aataggagcg tttggtgaag
     3181 tctgtctagc aagaaaagtc gatactaaag ctttgtatgc aacaaagact cttcgaaaga
     3241 aagacgttct gctccgaaat caggtggctc atgtgaaagc ggagagggat atcctagcag
     3301 aagccgacaa tgagtgggtg gtccgcctgt actactcttt ccaggacaag gacaacttgt
     3361 actttgtgat ggactacatt cctggggggg atatgatgag cctattaatt agaatgggca
     3421 tctttcctga aaatctggca cgattctaca tagcagaact tacctgtgca gttgaaagtg
     3481 ttcataaaat gggttttatt catagagata ttaaacctga taacattttg attgaccgtg
     3541 atggccatat taaattgact gactttggct tgtgcactgg cttcagatgg acacatgact
     3601 ccaagtacta ccagagtggg gatcacccac ggcaagatag catggatttc agtaacgaat
     3661 ggggagatcc ttccaattgt cggtgtgggg acagactgaa gccactggag cggagagctg
     3721 ctcgccagca ccagcgatgt ctagcccatt ctctggttgg gactcccaat tatattgcac
     3781 ctgaagtgct actgcgaaca ggatatacac agctgtgtga ctggtggagt gttggtgtta
     3841 ttctttttga aatgttggtg ggacaacctc ctttcttggc acaaacccca ttagaaacac
     3901 aaatgaaggt tatcaactgg caaacttctc ttcacatccc tcctcaagct aagctgagtc
     3961 ctgaagcctc tgacctcatt atcaaacttt gtcgaggacc agaagaccgc ctcggcaaga
     4021 acggtgctga tgagataaag gctcatccat tttttaagac catcgatttc tctagtgatc
     4081 tgagacagca gtctgcttca tacatcccta aaatcacgca tccaacagat acatccaatt
     4141 tcgaccctgt tgatcctgat aaattgtgga gcgatggcag cgaggaggaa aatatcagtg
     4201 acactctgaa cggatggtat aaaaatggga agcaccccga gcacgctttc tatgagttca
     4261 cctttcggag gttttttgat gacaatggct acccatataa ttatccaaag cctattgagt
     4321 atgaatacat tcattcacag ggctcagaac aacagtctga tgaagatgat caacacacaa
     4381 gctccgatgg aaacaaccga gatctagtgt atgttacgcg tacgcggccg ctcgagcaga
     4441 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     4501 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     4561 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     4621 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     4681 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     4741 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     4801 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     4861 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     4921 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     4981 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     5041 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     5101 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     5161 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     5221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6061 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     6121 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     6181 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     6241 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     6301 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     6361 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     6421 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     6481 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     6541 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     6601 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     6661 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     6721 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     6781 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     6841 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     6901 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     6961 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     7021 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     7081 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     7141 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     7201 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     7261 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     7321 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     7381 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     7441 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7501 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7561 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     7621 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     7681 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     7741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     7861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
