LOCUS       V040674                12087 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040674
VERSION     V040674
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..6767
                     /label="KAT6A(NM_006766)"
                     /note="KAT6A(NM_006766)"
                     /gene="KAT6A"
     CDS             6774..6839
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6863..7451
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7517..7565
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7767..8195
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8209..8538
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8605..9399
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9575..9696
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9745..9761)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9769..9785
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9793..9823)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9838..9859
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10147..10735)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10906..11766)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11767..11871)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggt aaaactcgca aacccgcttt
      781 atactgagtg gattttggag gccatcaaaa aagtgaaaaa gcagaaacag cgtccttcag
      841 aagaaaggat atgcaatgct gtgtcttcat cccatggctt ggatcgtaaa actgttttag
      901 aacaattgga gttgagtgtt aaagatggaa caattttaaa agtctcaaat aaaggactca
      961 attcctataa agatcctgat aatcctgggc gaatagcact tcctaagcct cggaaccatg
     1021 gaaaattgga taataaacaa aatgtggatt ggaataaact gataaagcgg gcagttgagg
     1081 gcttggcaga gtctggtggc tcaactttga aaagcattga acgttttttg aaaggtcaga
     1141 aggatgtgtc tgcattattc ggaggcagtg ctgcctctgg ctttcaccag cagttacgat
     1201 tggctatcaa acgtgccatt ggccacggca gactccttaa agatggacct ctttatcggc
     1261 tcaacactaa agcaaccaac gtggatggga aagagagttg tgagtctctt tcctgtttac
     1321 ctccagtgtc ccttcttcca catgaaaagg ataagccggt tgctgaacca atccccatct
     1381 gtagtttctg tcttggtaca aaagaacaaa accgagaaaa gaagccagag gaactcatct
     1441 cctgtgccga ctgtggcaac agtggccatc catcctgttt aaagttttcc cctgaactaa
     1501 cggttcgagt gaaggcctta cggtggcagt gcatcgagtg taaaacatgc agctcctgtc
     1561 gagatcaagg caaaaatgcg gataacatgc tcttttgtga ttcatgtgac cgaggttttc
     1621 acatggagtg ttgtgatccg ccactcaccc gtatgccaaa aggcatgtgg atatgtcaaa
     1681 tatgtcgacc taggaaaaaa ggacgaaaac ttctacaaaa gaaggcagca cagataaaac
     1741 ggcgctatac taatccaata ggacgtccaa aaaacaggtt aaagaaacaa aacacggtat
     1801 caaaaggtcc cttcagcaaa gttcgaactg gccctggaag gggtaggaaa cgaaaaatca
     1861 ctctttccag ccaatcagca tcatcatcat cagaagaagg atatttagag cggatagatg
     1921 gcttggactt ctgcagagat agcaatgtct ccttgaagtt caacaagaaa accaaagggc
     1981 tcattgatgg ccttaccaaa ttttttaccc cttcccctga tgggcggaaa gctcgggggg
     2041 aagtggtgga ctactctgag caatatcgaa tcagaaagag gggcaacagg aaatcaagca
     2101 cttcagattg gcccacagac aatcaggatg gctgggatgg caaacaagaa aatgaggagc
     2161 gactttttgg gagccaggaa atcatgactg agaaagatat ggaattattt cgtgatatcc
     2221 aagaacaagc actgcagaaa gttggagtga ctggtccccc tgatccacaa gtccgctgtc
     2281 cctctgtcat tgagtttggg aagtatgaaa ttcacacctg gtactcctcc ccatatcctc
     2341 aagaatactc aaggctgccc aaattgtatc tttgtgaatt ttgtctaaaa tatatgaaaa
     2401 gtagaactat tctgcagcag cacatgaaga aatgtggttg gttccatcct cctgccaatg
     2461 agatttacag aaagaataat atttctgtct ttgaggttga tgggaatgtg agtaccattt
     2521 attgtcaaaa cctgtgtctt ttggcaaagt tgtttcttga ccacaaaacc ctctattacg
     2581 atgtggagcc atttcttttt tatgtactaa cacagaatga tgtcaagggc tgccaccttg
     2641 ttggctactt ttctaaggaa aagcactgcc aacagaagta caatgtttcc tgtataatga
     2701 ttcttcctca ataccagcgt aagggctatg gcaggtttct catcgatttc agttatttgt
     2761 tatcaaagcg tgaaggccaa gcagggtctc cagagaaacc gttatctgat ctgggtcgtc
     2821 tttcctacat ggcatattgg aaaagtgtaa tattggagtg cctttatcac caaaatgaca
     2881 agcagatcag cattaagaag ttaagcaagt tgactggaat ctgccctcaa gacatcactt
     2941 ccacactcca ccacctacga atgctggact tccgtagtga ccaatttgtg attatccgcc
     3001 gggaaaaact tatccaggat cacatggcaa agcttcagct gaatttgcga cctgtagatg
     3061 tagatccaga atgtttgcgc tggactccag tcatagtgtc caactctgtg gtctcagagg
     3121 aggaagaaga ggaggctgag gaaggagaaa acgaagagcc acagtgccag gaaagagaat
     3181 tagagatcag tgtgggaaag tctgtgtctc atgagaacaa agaacaagat tcttattcag
     3241 tagaaagtga aaagaaacca gaagttatgg ctccagtcag ttctacacgt ttgagcaaac
     3301 aagtccttcc tcatgatagt cttcctgcaa atagccagcc atctcggagg ggccgctggg
     3361 ggaggaagaa cagaaaaacc caggaacgtt ttggtgataa agattctaaa ctgctcttgg
     3421 aagagacgtc ttcagctcct caggaacaat atggagaatg tggggagaaa tcagaagcca
     3481 cccaggaaca atacactgaa agtgaagaac agctggtggc ttctgaggag cagccaagcc
     3541 aggacgggaa acctgacctt cccaagagaa gactcagtga gggggttgag ccctggcgag
     3601 gacagctcaa gaaaagccct gaggctctga agtgcagatt aacagaagga agtgagaggc
     3661 tgccccgtcg ctacagtgag ggtgacaggg ctgtcctcag gggcttcagt gagagcagcg
     3721 aggaggagga ggagccggaa agccctcggt caagctcgcc accaattctc acaaagccca
     3781 cgctgaagcg aaagaaacca tttctccacc gaaggaggag agtccgaaag cgcaaacacc
     3841 acaatagcag tgtagtcaca gaaactattt ctgagaccac tgaagtgtta gatgaacctt
     3901 ttgaagattc tgactccgag aggccaatgc caagattaga acccacgttt gagatcgatg
     3961 aagaagagga ggaagaggat gaaaatgaac ttttccctag agaatacttc cgtcgtttgt
     4021 cttcgcagga tgtactcagg tgtcagtcct cttctaagag gaagtctaaa gatgaagaag
     4081 aagatgaaga gtcagatgat gctgatgaca ctcctatctt aaagccagta tctcttttgc
     4141 gaaaacgtga tgtgaagaat tctcctcttg agccagatac atccacacct ttgaaaaaga
     4201 aaaagggatg gcccaaaggc aagagccgca aaccaatcca ctggaagaaa agacctggtc
     4261 gaaaaccagg atttaagttg agtcgggaaa tcatgccagt ttctactcaa gcatgcgtca
     4321 ttgagcccat cgtttccatt cctaaagctg gacgtaaacc caagatccag gagagtgaag
     4381 aaactgttga gccaaaagaa gacatgcccc tacccgagga gaggaaggag gaggaggaga
     4441 tgcaagcaga ggcagaagag gctgaagagg gtgaggaaga ggatgcagcc agcagtgaag
     4501 tcccagcagc ctctccagca gacagcagca atagtcctga gaccgaaacc aaggagcctg
     4561 aggtggagga ggaagaagag aagccccgtg tctcagagga gcagaggcag tcagaggagg
     4621 agcagcagga attagaggag ccagagccag aggaggagga agatgcagct gcagagactg
     4681 cccagaatga cgaccacgac gctgatgatg aggatgatgg ccacctggag tccacaaaga
     4741 aaaaggagct agaggaacag cccacgaggg aagatgtcaa ggaggagcct ggtgttcaag
     4801 agtctttttt agatgctaat atgcagaaga gtagggaaaa gataaaggat aaagaggaaa
     4861 ccgagctgga ttccgaagag gagcagcctt cccatgacac gtccgtggtg tcagagcaga
     4921 tggctgggtc tgaggacgac cacgaagaag actcccacac taaggaagag ttaatcgaat
     4981 taaaagagga ggaagagatt cctcatagtg agctggatct ggaaactgta caggcagtgc
     5041 agtctttgac tcaagaagaa agcagtgagc atgagggcgc ctaccaggac tgtgaggaaa
     5101 ctcttgcggc gtgtcagacc ctgcagagtt acacccaggc tgacgaggac cctcagatgt
     5161 ccatggttga agactgtcat gcgtcagaac ataatagccc tatctcctcc gttcagtctc
     5221 accccagcca gtcagtccgt tcggtcagca gtcccaacgt gcctgccctt gagagtggct
     5281 acacccagat cagcccagaa caaggatccc tgtccgcacc ctctatgcag aacatggaga
     5341 ccagccccat gatggatgtg ccttccgtat cagaccactc tcagcaggtg gtggacagcg
     5401 gcttcagtga cctgggcagc attgagagca ccactgaaaa ctatgagaac ccaagcagtt
     5461 acgactccac gatgggcggc agcatctgtg ggaacagctc ttcccagagc agctgctcct
     5521 acggtgggct gtcgtcctcc agcagcctca cccagagcag ctgtgtggtc actcagcaga
     5581 tggccagcat gggcagcagc tgcagcatga tgcagcagag cagcgtccag cctgctgcca
     5641 actgcagcat caagtcacct cagagctgcg tggtggagag gcctcccagt aaccagcagc
     5701 agcagccgcc accaccgcct ccacagcagc cacagccgcc gccgccacaa ccacaaccag
     5761 caccacagcc tccaccaccc cagcagcagc cgcaacagca gccgcagcct cagccccagc
     5821 agcctccacc cccaccccct ccccagcagc agcccccgct gtcacagtgt agtatgaata
     5881 acagtttcac cccagctcct atgatcatgg agataccaga atctggaagc actgggaaca
     5941 taagtatcta tgagaggatt ccaggggatt ttggtgccgg cagctactct caaccatcag
     6001 ccaccttcag cctagccaag ctgcagcagc tgaccaacac cattatggac cctcatgcca
     6061 tgccttatag ccattctcct gctgtgactt cctatgcaac cagtgtttct ctgtccaata
     6121 caggactggc tcagctggct ccatctcatc ccttagctgg gactcctcaa gcacaagcca
     6181 ccatgacgcc acccccaaac ttggcatcca ctaccatgaa cctcacatct cctctgcttc
     6241 agtgcaacat gtctgccacc aacattggca ttcctcacac gcagagattg caagggcaaa
     6301 tgccagtgaa ggggcacatt tccatccgct ccaagtctgc gccactgccc tctgcggctg
     6361 ctcaccagca gcagctgtat ggccgtagcc catcggcagt tgccatgcag gctggccctc
     6421 gcgcactggc tgttcagcgt ggcatgaaca tgggggttaa tctgatgcct actcccgcct
     6481 ataatgtcaa ttccatgaat atgaacacct tgaatgccat gaacagctat cgaatgacac
     6541 agcccatgat gaacagcagt taccatagta accctgccta catgaaccag acagcacagt
     6601 atcctatgca gatgcagatg ggaatgatgg ggagccaggc ctatacccag cagcctatgc
     6661 agcctaaccc tcatgggaac atgatgtaca caggcccctc ccatcacagc tacatgaacg
     6721 ctgctggcgt gcccaagcag tcactcaacg gaccttacat gagaagagga tccgactaca
     6781 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     6841 agtgagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6901 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6961 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7021 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7081 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7141 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7201 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7261 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7321 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7381 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7441 cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     7501 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     7561 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     7621 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     7681 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     7741 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7801 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7861 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7921 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7981 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8041 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8101 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
     8161 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8221 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8281 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8341 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8401 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8461 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8521 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     8581 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     8641 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     8701 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     8761 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     8821 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     8881 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     8941 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     9001 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     9061 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     9121 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     9181 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     9241 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     9301 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     9361 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     9421 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     9481 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     9541 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     9601 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9661 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9721 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10321 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10561 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    10621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11881 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtcgact
    11941 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    12001 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    12061 gacaattgca tgaagaatct gcttagg
//
