LOCUS       V031240                 6372 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031240
VERSION     V031240
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2519
                     /label="Irf5(NM_012057)"
                     /note="Irf5(NM_012057)"
                     /gene="Irf5"
     CDS             2538..2567
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2586..2609
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2629..2645)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2656..3278
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3427..4015)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4344..4391
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4623..5417)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5452..5809)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5811..5915)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5942..6370
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaccactca gccccaggga ttcccccacc accccgccgt gtgaggctga
     1081 agccctggtt ggtggcccag gtgaacagct gccagtaccc agggcttcag tgggtcaacg
     1141 gggaaaagaa actcttctat ataccctggc gccatgccac gaggcatggt cccagccagg
     1201 atggggacaa caccatcttc aaggcctggg ctaaagagac agggaagtac actgaagggg
     1261 tggatgaggc tgacccagcc aagtggaagg ccaacctgcg ctgtgccctt aacaaaagcc
     1321 gtgacttcca gctgttctat gatggccctc gggacatgcc acctcagccg tacaagatct
     1381 acgaggtctg ctccaacggc cctgctccca cagagagcca acccactgat gattacgttc
     1441 tgggagaaga ggaggaggag gaagaggaag agctccagag aatgctacca ggcctgagca
     1501 tcacagagcc tgcgctacct gggcctccca acgcacccta ttccttaccc aaagaagaca
     1561 ccaagtggcc acctgctctc cagccacctg tagggctggg tccccctgtc ccagacccaa
     1621 atctcctggc ccctccctct ggaaatcctg ctggcttcag gcagcttctc cctgaggtcc
     1681 tggagcctgg acctctggct tccagccagc cccctacaga accactcttg cctgacctgc
     1741 tgatcagccc ccacatgttg cctttgacgg acctagagat caagttccag taccggggac
     1801 gcgcaccccg gaccctcacc atcagcaacc cacaaggctg caggctcttc tacagccagc
     1861 tagaggctac ccaggagcaa gtggaactct ttggccctgt gaccctggag caagtgcgct
     1921 tccctagccc agaggacatc cccagtgaca agcagcgttt ctatacgaac cagctgctag
     1981 atgtcctgga ccgtgggctc atcctgcagc tgcagggcca ggacctgtac gccatccgtc
     2041 tgtgccagtg taaggtgttc tggagtgggc cctgcgcctt ggcccatggc tcctgcccca
     2101 accccattca gcgggaagtc aagacgaagc tctttagcct agagcagttt ctcaatgagc
     2161 tcatcctgtt ccagaagggc cagactaata ccccaccacc ttttgagatc ttcttttgct
     2221 ttggagaaga atggcctgat gtcaaacccc gagagaagaa gctcattact gtacaggtgg
     2281 tacctgttgc agcccggttg ctgctggaga tgttctcagg ggagctttct tggtcggcag
     2341 acagcatccg actgcagatc tcaaacccgg atctcaaaga ccacatggta gagcagttta
     2401 aagagcttca tcacctctgg cagtcccagc agcaattgca gcccatggtc caggcccctc
     2461 ctgtggcagg cctcgatgca agccaggggc cctggcccat gcacccagtt ggcatgcaaa
     2521 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     2581 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     2641 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     2701 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     2761 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     2821 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     2881 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     2941 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     3001 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3061 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3121 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     3181 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     3241 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     3301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4141 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     4201 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     4261 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     4321 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     4381 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     4441 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4501 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4561 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4621 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4681 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4741 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     4801 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     4861 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     4921 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     4981 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5041 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5101 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5161 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5221 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5281 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5341 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5401 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     5461 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     5521 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     5581 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     5641 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     5701 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5761 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     5821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5941 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6001 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6061 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6301 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6361 aacgcgaatt tt
//
