LOCUS V030235 7311 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030235 VERSION V030235 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..3458 /label="Il4ra(NM_001008700)" /note="Il4ra(NM_001008700)" /gene="Il4ra" CDS 3477..3506 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 3525..3548 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(3568..3584) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 3595..4217 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(4366..4954) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 5283..5330 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(5562..6356) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(6391..6748) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(6750..6854) /gene="bla" /label="AmpR promoter" rep_origin 6881..7309 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggggcggctt tgcaccaagt tcctgacctc tgtgggctgt ctgattttgc 1081 tgttggtgac tggatctggg agcatcaagg tcctgggtga gcccacctgc ttctctgact 1141 acatccgcac ttccacgtgt gagtggttcc tggatagcgc tgtggactgc agttctcagc 1201 tctgcctaca ctacaggctg atgttcttcg agttctctga aaacctcaca tgcatcccga 1261 ggaacagtgc cagcactgtg tgtgtgtgcc acatggaaat gaataggccg gtccaatcag 1321 acagatacca gatggaactg tgggctgagc acagacagct gtggcagggc tccttcagcc 1381 ccagtggtaa tgtgaagccc ctagctccag acaacctcac actccacacc aatgtgtccg 1441 acgaatggct gctgacctgg aataacctgt acccatcgaa caacttactg tacaaagacc 1501 tcatctccat ggtcaacatc tccagagagg acaaccctgc agaattcata gtctataatg 1561 tgacctacaa ggaacccagg ctgagcttcc cgatcaacat cctgatgtca ggggtctact 1621 atacggcgcg tgtgagggtc agatcccaga tactcactgg cacctggagt gagtggagtc 1681 ctagcatcac gtggtacaac cacttccagc tgcccctgat acagcgcctt ccactggggg 1741 tcaccatctc ctgcctctgc atcccgttgt tttgcctgtt ctgttacttc agcattacca 1801 agattaagaa gatatggtgg gaccagattc ccaccccagc acgcagtccc ttggtggcca 1861 tcatcattca ggatgcacag gtgcccctct gggataagca gacccgaagc caggagtcaa 1921 ccaagtaccc gcactggaaa acttgtctag acaagctgct gccttgcttg ctgaagcaca 1981 gagtaaagaa gaagacagac ttcccgaagg ctgccccaac caagtctctc cagagtcctg 2041 gaaaggcagg ctggtgtccc atggaggtca gcaggaccgt cctctggcca gagaatgtta 2101 gtgtcagtgt ggtgcgctgt atggagctgt ttgaggcccc agtacagaat gtggaggagg 2161 aagaagatga gatagtcaaa gaggacctga gcatgtcacc tgagaacagc ggaggctgcg 2221 gcttccagga gagccaggca gacatcatgg ctcggctcac tgagaacctg ttttccgact 2281 tgttggaggc tgagaatggg ggccttggcc agtcagcctt ggcagagtca tgctcccctc 2341 tgccttcagg aagtgggcag gcttctgtat cctgggcctg cctccccatg gggcccagtg 2401 aggaggccac atgccaggtc acagagcagc cttcacaccc aggccctctt tcaggcagcc 2461 cagcccagag tgcacctact ctggcttgca cgcaggtccc acttgtcctt gcagacaatc 2521 ctgcctaccg gagttttagt gactgctgta gcccggcccc aaatcctgga gagctggctc 2581 cagagcagca gcaggctgat catctggaag aagaggagcc tccaagcccg gctgaccccc 2641 attcttcagg gccaccaatg cagccagtgg agagctggga gcagatcctt cacatgagtg 2701 tcctgcagca tggggcagct gctggctcca ccccagcccc tgccggtggc taccaggagt 2761 ttgtgcaggc agtgaagcag ggtgccgccc aggatcctgg ggtgcctggt gtcaggcctt 2821 ctggagaccc cggttacaag gccttctcga gcctgctcag cagcaatggc atccgcgggg 2881 acacagcagc agcggggact gacgatgggc atggaggcta caagcccttc cagaatcctg 2941 ttcctaacca gtcccctagc tccgtgccct tatttacttt cggactagac acggagctgt 3001 cacccagtcc tctgaactca gacccaccca aaagcccccc agaatgcctt ggtctggagc 3061 tggggctcaa aggaggtgac tgggtgaagg cccctcctcc tgcagatcag gtgcccaagc 3121 cctttgggga tgacctgggc tttggtattg tgtactcgtc cctcacttgc cacttgtgtg 3181 gccacctgaa gcaacaccac agccaggagg aaggtggcca gagccccatc gttgctagcc 3241 ctggctgtgg ctgctgctac gatgacagat caccatccct ggggagcctc tcgggggcct 3301 tggaaagctg tcctgaggga ataccaccag aagccaacct catgtcagca cccaagacac 3361 cctcaaactt gtcaggggag ggcaagggcc ctggtcactc tcctgttccc agccagacga 3421 ccgaggtgcc tgtgggcgcc ctgggcattg ctgtttctac gcgtacgcgg ccgctcgagc 3481 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg 3541 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg 3601 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc 3661 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat 3721 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt 3781 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact 3841 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga 3901 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc 3961 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct 4021 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta 4081 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa 4141 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag 4201 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc 4261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 4321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 4381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 4441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 4501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 4561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 5041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 5101 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg 5161 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc 5221 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt 5281 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg 5341 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa 5401 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 5461 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 5521 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 5581 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 5641 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 5701 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 5761 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 5821 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 5881 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 5941 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 6001 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 6061 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 6121 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 6181 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 6241 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 6301 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 6361 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc 6421 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 6481 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 6541 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 6601 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 6661 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 6721 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt 6781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 6841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca 6901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 7021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 7081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 7141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 7201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 7261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t //