LOCUS       V030235                 7311 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030235
VERSION     V030235
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3458
                     /label="Il4ra(NM_001008700)"
                     /note="Il4ra(NM_001008700)"
                     /gene="Il4ra"
     CDS             3477..3506
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3568..3584)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3595..4217
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4366..4954)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5283..5330
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5562..6356)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6391..6748)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6750..6854)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6881..7309
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggggcggctt tgcaccaagt tcctgacctc tgtgggctgt ctgattttgc
     1081 tgttggtgac tggatctggg agcatcaagg tcctgggtga gcccacctgc ttctctgact
     1141 acatccgcac ttccacgtgt gagtggttcc tggatagcgc tgtggactgc agttctcagc
     1201 tctgcctaca ctacaggctg atgttcttcg agttctctga aaacctcaca tgcatcccga
     1261 ggaacagtgc cagcactgtg tgtgtgtgcc acatggaaat gaataggccg gtccaatcag
     1321 acagatacca gatggaactg tgggctgagc acagacagct gtggcagggc tccttcagcc
     1381 ccagtggtaa tgtgaagccc ctagctccag acaacctcac actccacacc aatgtgtccg
     1441 acgaatggct gctgacctgg aataacctgt acccatcgaa caacttactg tacaaagacc
     1501 tcatctccat ggtcaacatc tccagagagg acaaccctgc agaattcata gtctataatg
     1561 tgacctacaa ggaacccagg ctgagcttcc cgatcaacat cctgatgtca ggggtctact
     1621 atacggcgcg tgtgagggtc agatcccaga tactcactgg cacctggagt gagtggagtc
     1681 ctagcatcac gtggtacaac cacttccagc tgcccctgat acagcgcctt ccactggggg
     1741 tcaccatctc ctgcctctgc atcccgttgt tttgcctgtt ctgttacttc agcattacca
     1801 agattaagaa gatatggtgg gaccagattc ccaccccagc acgcagtccc ttggtggcca
     1861 tcatcattca ggatgcacag gtgcccctct gggataagca gacccgaagc caggagtcaa
     1921 ccaagtaccc gcactggaaa acttgtctag acaagctgct gccttgcttg ctgaagcaca
     1981 gagtaaagaa gaagacagac ttcccgaagg ctgccccaac caagtctctc cagagtcctg
     2041 gaaaggcagg ctggtgtccc atggaggtca gcaggaccgt cctctggcca gagaatgtta
     2101 gtgtcagtgt ggtgcgctgt atggagctgt ttgaggcccc agtacagaat gtggaggagg
     2161 aagaagatga gatagtcaaa gaggacctga gcatgtcacc tgagaacagc ggaggctgcg
     2221 gcttccagga gagccaggca gacatcatgg ctcggctcac tgagaacctg ttttccgact
     2281 tgttggaggc tgagaatggg ggccttggcc agtcagcctt ggcagagtca tgctcccctc
     2341 tgccttcagg aagtgggcag gcttctgtat cctgggcctg cctccccatg gggcccagtg
     2401 aggaggccac atgccaggtc acagagcagc cttcacaccc aggccctctt tcaggcagcc
     2461 cagcccagag tgcacctact ctggcttgca cgcaggtccc acttgtcctt gcagacaatc
     2521 ctgcctaccg gagttttagt gactgctgta gcccggcccc aaatcctgga gagctggctc
     2581 cagagcagca gcaggctgat catctggaag aagaggagcc tccaagcccg gctgaccccc
     2641 attcttcagg gccaccaatg cagccagtgg agagctggga gcagatcctt cacatgagtg
     2701 tcctgcagca tggggcagct gctggctcca ccccagcccc tgccggtggc taccaggagt
     2761 ttgtgcaggc agtgaagcag ggtgccgccc aggatcctgg ggtgcctggt gtcaggcctt
     2821 ctggagaccc cggttacaag gccttctcga gcctgctcag cagcaatggc atccgcgggg
     2881 acacagcagc agcggggact gacgatgggc atggaggcta caagcccttc cagaatcctg
     2941 ttcctaacca gtcccctagc tccgtgccct tatttacttt cggactagac acggagctgt
     3001 cacccagtcc tctgaactca gacccaccca aaagcccccc agaatgcctt ggtctggagc
     3061 tggggctcaa aggaggtgac tgggtgaagg cccctcctcc tgcagatcag gtgcccaagc
     3121 cctttgggga tgacctgggc tttggtattg tgtactcgtc cctcacttgc cacttgtgtg
     3181 gccacctgaa gcaacaccac agccaggagg aaggtggcca gagccccatc gttgctagcc
     3241 ctggctgtgg ctgctgctac gatgacagat caccatccct ggggagcctc tcgggggcct
     3301 tggaaagctg tcctgaggga ataccaccag aagccaacct catgtcagca cccaagacac
     3361 cctcaaactt gtcaggggag ggcaagggcc ctggtcactc tcctgttccc agccagacga
     3421 ccgaggtgcc tgtgggcgcc ctgggcattg ctgtttctac gcgtacgcgg ccgctcgagc
     3481 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     3541 acgataaggt ttaaacggcc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg
     3601 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3661 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     3721 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     3781 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     3841 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     3901 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     3961 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     4021 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta
     4081 aaataactat accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa
     4141 ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag
     4201 tagatgcctg ttgaattggg tacgcggcca gcggcgagcg gtatcagctc actcaaaggc
     4261 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4321 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4381 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4441 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4501 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4561 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4621 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4681 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4741 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4801 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4861 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4921 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4981 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5041 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5101 atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg
     5161 gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc
     5221 ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt
     5281 atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     5341 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa
     5401 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     5461 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     5521 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     5581 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     5641 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     5701 tgatagcgat ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     5761 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     5821 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     5881 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     5941 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     6001 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     6061 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     6121 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     6181 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     6241 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     6301 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     6361 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     6421 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     6481 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     6541 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     6601 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     6661 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     6721 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     6781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt t
//
