LOCUS       V027943                 7032 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027943
VERSION     V027943
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3179
                     /label="Ikbke(NM_019777)"
                     /note="Ikbke(NM_019777)"
                     /gene="Ikbke"
     CDS             3198..3227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3246..3269
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3289..3305)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3316..3938
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4087..4675)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5004..5051
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5283..6077)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6112..6469)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6471..6575)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6602..7030
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcagagtacc actaactacc tgtggcatac tgatgacctg ctagggcagg
     1081 gggccactgc cagtgtgtac aaggcccgaa acaagaaatc cggggaggtg gttgctgtaa
     1141 aggtcttcaa ctcagccagc tatcggcgac ctcctgaggt tcaggtgagg gagtttgagg
     1201 tcctgcggag gctgaatcac cagaacatcg tgaagctatt cgcagtggag gaaacgggag
     1261 gcagccggca gaaggtgcta atcatggagt actgctccag tgggagcctg ctgagcgtgc
     1321 tggaagaccc tgagaacacg ttcgggcttt ctgaagagga gttcctagtg gtgctgcgct
     1381 gtgtggtggc tggcatgaac cacctgcggg agaatggcat tgtccatcgg gacatcaaac
     1441 ctgggaacat catgcgcctg gtgggcgagg aggggcagag catctataag ctgtctgact
     1501 tcggggctgc ccgcaagctg gacgatgatg agaagtttgt ttctgtctat ggtacagagg
     1561 aatacctgca ccctgacatg tatgagcgtg cagtgctgcg caaaccccag caaaaggcat
     1621 ttggtgtgac tgtggatctc tggagtattg gggtgaccct gtaccacgca gccacaggca
     1681 gtctgccctt catccccttc ggtgggcccc ggcgcaacaa agagatcatg tacagaatca
     1741 ccacagagaa gccagccggg gccatttcag ggactcagaa gcaggaaaat ggtcccttgg
     1801 agtggagcta cagcctcccc atcacctgta gactgtccat gggactgcag aaccagctgg
     1861 tgcccatcct ggccaacatc ctggaggtgg aagaggataa gtgctggggc tttgatcagt
     1921 tcttcgcgga gaccagtgac attctgcagc gaacggtcat ccacgtcttt tccctacccc
     1981 aggccgtttt gcatcatgtc tacatccacg cccacaacac gattgccatc tttttggagg
     2041 ctgtatatga gcagaccaac gtgaccccca aacaccagga gtacctcttc gagggtcacc
     2101 cttgtgtcct tgagccaagc ctctcagccc agcacatcgc ccacacagct gccagcagcc
     2161 ctctaactct gttcagcatg tccagcgaca cacctaaggg gctggccttc agggaccctg
     2221 ctctggatgt cccaaagttc gtccctaagg ttgacctaca ggccgattac agcacagcta
     2281 agggggtgct gggcgctggc taccaggccc tgtggctggc gcgggtcctg ctggatggac
     2341 aggcgttgat gcttcggggg ttacattggg tcctggaggt gcttcaggac acgtgccagc
     2401 agacactgga ggtcacacgg acagccctcc tctacctcag cagcagcctg ggcactgaaa
     2461 ggttcagcag tggagcgggg atgcctgacg tccaggaacg aaaggaggcc acagagctaa
     2521 gaaccaggct gcagactctc tcagagatcc tgtctaaatg ttcccacaat gtcacagaaa
     2581 cccaaaggag cctgagctgt ctgggtgaag agcttttaaa gaaccgggac cagattcatg
     2641 aggataacaa aagtatccag aagattcagt gttgtttgga caagatgcac ttcatctaca
     2701 aacagttcaa gaaatccagg atgaggccag ggctcagcta caatgaggag cagatccaca
     2761 agctggataa ggtaaatttc agtcatctag ccaagaggct gctgcaggtg ttccaggagg
     2821 agtgtgtgca gacgtatcag gtgtcgctgg tcacacacgg caagcggatg aggcaggtgc
     2881 agagggccca gaaccacctg catctcattg gccactctgt ggccacctgt aactcggaag
     2941 cccggggagc ccaggagagt ctgaacaaga tctttgatca gctccttctg gacagagctt
     3001 ccgaacaggg agctgaggtg tcaccgcaac ctatggctcc tcatcccggc cctgatccga
     3061 aggacctggt cttccacatg caggagcttt gtaatgatat gaagctattg gcctttgatc
     3121 tccaggacaa caaccgactc atcgaacggt tacatagagt tccatcggca ccagatgtca
     3181 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     3241 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     3301 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3361 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3421 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3481 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3541 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     3601 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     3661 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     3721 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3781 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     3841 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     3901 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     3961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4801 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     4861 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     4921 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     4981 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5041 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5101 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5161 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5221 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     5281 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     5341 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     5401 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     5461 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5521 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     5581 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5641 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5701 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5761 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5821 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5881 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5941 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6001 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6061 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6121 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6181 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6241 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     6301 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     6361 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     6421 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     6481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6601 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6661 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6721 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6781 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6841 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6901 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6961 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7021 aacgcgaatt tt
//
