LOCUS       V042842                 9274 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042842
VERSION     V042842
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3933
                     /label="Hdac4(NM_207225)"
                     /note="Hdac4(NM_207225)"
                     /gene="Hdac4"
     CDS             3940..3966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3970..3996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4000..4026
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4050..4638
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4704..4752
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4954..5382
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5396..5725
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5792..6586
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6762..6883
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6932..6948)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6956..6972
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6980..7010)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7025..7046
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7334..7922)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8093..8953)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8954..9058)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgag ctcccaaagc
      721 catccagatg gactttctgg ccgagaccag cctgtggagc tgctgaatcc tgcccgtgtg
      781 aaccacatgc ccagcacggt ggacgtggct acagcgctgc ctctgcaagt ggcccctaca
      841 gcagtaccca tggacctgcg cttggaccac cagttctcac tgcccttgga acctgcattg
      901 cgggagcagc aactgcagca ggaactccta gcactgaaac agaagcagca gatccagcgg
      961 cagatactca ttgcagagtt ccagcgtcaa catgagcagt tgtcccgaca gcatgaggca
     1021 cagttgcatg aacatatcaa gcagcagcag gagatgctgg ccatgaagca ccagcaggag
     1081 ctgctggagc accagcggaa actggagcgg caccggcaag agcaggagct ggagaagcag
     1141 caccgtgagc agaagctgca gcagctcaag aacaaggaga agggcaaaga gagtgctgtg
     1201 gcgagcacag aggtgaagat gaagctgcag gagtttgttc tcaacaagaa gaaggctcta
     1261 gcccaccgga acctgaacca ctgcatttcc agcgatcccc gctactggta tgggaagaca
     1321 cagcacagct cccttgacca gagctctcca ccccagagtg gggtgtcagc ctcctacaac
     1381 caccccgtct tgggaatgta cgacgccaaa gatgacttcc ctcttaggaa aacagcttct
     1441 gaacctaacc tgaaattacg ctcaaggctt aagcagaaag tagctgagag acggagcagc
     1501 cccctgttgc gcaggaaaga tggccctgtg gccactgctc taaaaaagcg acccctggat
     1561 gttacagact ccgcatgcag cagcgcccct ggctccggtc ccagctctcc aaatagcagc
     1621 tctggcaacg tcagcactga gaatggcatc gcacccactg tgcccagcgc tccagctgag
     1681 acgagcttgg cacacagact tgtgactcga gaaggctcag tcgccccact tcctctctac
     1741 acgtcaccat ccttacccaa catcaccttg ggacttcctg ccactggccc tgccgctggt
     1801 gcggcaggtc agcaggatgc tgagaggctt gctctcccag ctctccagca gcggatcttg
     1861 ttccctggga cccacctcac cccgtacctg agcacctcgc ccctggagag ggacggtgca
     1921 gcagctcaca accccctcct gcagcacatg gtcctgctgg agcagccacc cacccagaca
     1981 ccccttgtca caggcctggg ggcgctgccc ctccactcac agtccctggt tggtgcggac
     2041 agggtgtccc catccattca caagctgcgg cagcaccgcc ctctggggcg cacgcagtca
     2101 gcacccctgc cgcagaacgc acaggccctg cagcacctgg tgatccagca gcagcaccag
     2161 cagttcctgg agaagcacaa gcaacagttc cagcagcagc agctgcacct cagcaagata
     2221 atctccaaac ctagtgagcc acctcggcag cctgagagcc acccagagga gacagaggag
     2281 gagctccgtg agcaccaggc cttgctggat gagccctacc tagatcggct acctgggcag
     2341 aaggagccct ccctggctgg tgtgcaggtg aagcaggagc ccattgagag tgaggaggaa
     2401 gaagcggagg ccactcgaga gacagagccc ggccagcgcc cagccactga gcaggagctg
     2461 ctcttcagac agcaagccct cctactggag cagcagagga tccaccagtt aagaaactac
     2521 caggcatcta tggaggctgc tggcatccct gtgtcatttg gcagccacag acctctgtct
     2581 cgggcacagt cctccccagc atctgccacc ttccccatgt cagtccagga gccccccacc
     2641 aagccaaggt tcaccacagg tcttgtgtat gacacactga tgttgaagca tcagtgcacc
     2701 tgtgggaaca ccaacagcca cccggagcat gctgggagga tccagagcat ctggtcccgc
     2761 ctgcaggaga ctggactccg tggcaagtgt gagtgcatcc gtggacgcaa ggccacattg
     2821 gaggagctgc agacagtgca ctcggaggcc cacacactcc tctacggcac aaatcctctc
     2881 aacagacaga aactggacag ctcgctgacc tcagtgttcg tcaggcttcc ttgtggtggt
     2941 gttggggtgg atagcgacac catatggaat gaggtgcact cgtctggggc agcccgcctg
     3001 gctgtaggct gtgtagtgga gctggtcttc aaggtggcca cgggagagct aaagaatggc
     3061 tttgctgtgg ttcgtccccc aggacaccat gccgaggaga gcacacccat gggtttctgc
     3121 tactttaact ccgtggcagt tgcagccaaa cttctccagc agaggctgaa tgtgagcaag
     3181 atcctcattg tagactggga tgtacatcat gggaatggga cccagcaggc cttctacaat
     3241 gaccccaatg ttctctacat gtccctgcac cgctatgacg atgggaactt cttcccagga
     3301 agtggagcac cagatgaggt gggcacaggg ccaggcgtgg gtttcaatgt caacatggct
     3361 ttcacgggtg gcctcgaacc ccccatggga gacgctgagt acttggcagc cttcagaacg
     3421 gtggttatgc ctatcgcaaa tgagtttgcc ccagatgtgg tactggtgtc atcgggcttc
     3481 gatgctgtgg agggccaccc cacacctctt ggagggtaca atctctctgc caaatgtttt
     3541 gggtacttga caaaacagct gatgggctta gctggtggcc ggcttgtgct ggcccttgag
     3601 ggaggccatg acctgacagc catctgtgat gcttctgaag cctgcgtgtc tgctctgctg
     3661 ggaaacgagc ttgagcctct gccagaaaag gttctacatc agagacccaa tgccaatgct
     3721 gtccactcca tggagaaagt gatggacatc cacagcaagt actggcgctg cctgcagcgt
     3781 ctgtcctcca cggtggggca ctctctgatt gaggcgcaaa agtgtgagaa ggaagaagct
     3841 gagacagtca ccgccatggc ctcgctgtct gtaggcgtca aacctgctga gaagagatct
     3901 gaggaggagc ccatggagga ggaaccacca ctgctcgagt acccatacga cgtacctgat
     3961 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     4021 tacgcatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     4081 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4141 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4201 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4261 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4321 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4381 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4441 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4501 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4561 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4621 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     4681 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     4741 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4801 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     4861 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     4921 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4981 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     5041 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5101 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5161 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5221 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5281 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     5341 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5401 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5461 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5521 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5641 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5701 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5761 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5821 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5881 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5941 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     6001 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6061 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6121 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6181 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6241 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6301 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6361 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6421 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6481 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6541 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6601 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6661 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6721 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6901 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7501 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7741 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8221 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8701 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9061 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9121 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9181 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9241 cttgaccgac aattgcatga agaatctgct tagg
//
