LOCUS       V019416                 9041 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019416
VERSION     V019416
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             665..3691
                     /label="HLTF(NM_001318935)"
                     /note="HLTF(NM_001318935)"
                     /gene="HLTF"
     CDS             3707..3733
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3737..3763
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3767..3793
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3817..4405
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4471..4519
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4721..5149
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5163..5492
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5559..6353
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6529..6650
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6699..6715)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6723..6739
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6747..6777)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6792..6813
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7101..7689)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7860..8720)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8721..8825)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgc
      661 caccatgtcc tggatgttca agagggatcc agtttggaag tacttgcaga ctgtccagta
      721 tggagttcat ggaaattttc cacgcctctc atatccaact ttctttccac gttttgaatt
      781 ccaagatgtt atccctccag atgactttct aactagtgat gaagaagtag attccgtttt
      841 atttggaagt ttgagaggtc atgtggttgg actacgctat tacacgggag tagttaataa
      901 taatgaaatg gttgcattac aacgagatcc taataaccct tatgataaga atgcaattaa
      961 agtaaacaat gtgaatggaa atcaagttgg ccatttaaag aaagagcttg caggtgcttt
     1021 ggcctatatc atggacaaca aattggcaca aattgaaggg gtagttcctt ttggtgcaaa
     1081 caatgctttt accatgcctc tgcatatgac tttttgggga aaagaagaaa atagaaaagc
     1141 ggtttcagat cagttgaaga aacatggatt taaattgggt cctgcaccaa aaactttagg
     1201 attcaatttg gaaagtggtt ggggctctgg aagagctgga ccaagctata gtatgccagt
     1261 gcatgctgca gtacagatga caactgaaca gcttaaaaca gaatttgaca aattgtttga
     1321 agatttaaaa gaagatgata aaacccatga aatggaacca gctgaggcta ttgaaacacc
     1381 actgcttcca catcaaaaac aagctctagc ttggatggtg tcacgggaaa atagcaaaga
     1441 acttccacca ttctgggaac agcgaaatga cttatactat aacacaataa caaatttttc
     1501 tgagaaggac cgaccagaaa atgtccatgg aggaatttta gctgatgata tgggtttggg
     1561 taaaactctt acggccattg cagtaatcct taccaacttc catgatggca gacctcttcc
     1621 tattgaaaga gttaaaaaga atctactgaa gaaggaatat aatgttaacg atgactctat
     1681 gaaacttgga ggaaacaata ccagtgaaaa ggcagatgga ctaagcaaag acgcatctag
     1741 atgtagtgaa caacccagta tttcagatat caaggagaag agtaagtttc gcatgtcaga
     1801 attgtctagc tcccgcccca aaagaagaaa aactgctgtc cagtacatag aaagcagtga
     1861 ttcagaggaa attgaaacaa gtgaattgcc gcagaaaatg aaaggcaaac tgaaaaatgt
     1921 acagtctgaa actaaaggca gggcgaaagc aggatcttct aaggttatag aagatgtggc
     1981 atttgcatgt gcattaactt catctgttcc tacaacaaaa aagaaaatgt tgaaaaaggg
     2041 agcttgtgca gtggaggggt caaagaaaac tgatgttgag gagagaccaa gaacaacact
     2101 gatcatctgt ccgctttctg tgttaagcaa ctggattgac cagtttggac aacatataaa
     2161 atcagatgta cacttgaatt tttatgttta ttatggtcct gatcgtatta gagaaccggc
     2221 cttactttca aaacaggata ttgttttgac tacgtataat attttaactc atgactatgg
     2281 aactaaagga gatagtccat tacatagcat aaggtggcta agagtgatcc tggatgaagg
     2341 acatgccata cgaaatccaa atgctcagca gacaaaagct gtacttgact tagaatcaga
     2401 aagaagatgg gttttgacag gtactccaat ccagaattct ttaaaggact tgtggtctct
     2461 tctttccttt ttaaaactta aaccatttat tgatagagaa tggtggcata gaacaataca
     2521 gcgtcctgtc acaatgggag atgaaggagg acttaggcgt ttacagtccc taattaaaaa
     2581 tattacactt agaagaacaa agacaagcaa aattaaagga aaacctgttt tggagttacc
     2641 agaacgtaaa gtatttattc agcacattac actttcagat gaagagagaa agatttatca
     2701 gtctgtgaaa aatgaaggca gagccactat tggaaggtat tttaatgaag ggactgtcct
     2761 ggcacattat gcagatgtcc tgggtctttt gcttagactg cggcaaattt gttgccatac
     2821 ttaccttctt acaaatgcag tgtcttccaa tggcccctca ggaaatgata cacctgaaga
     2881 actgagaaag aagttaataa ggaagatgaa gttaattctg agctcaggtt cagatgagga
     2941 atgtgcaatt tgcctggatt ctttaacagt tcctgtgata acacattgtg cacatgtatt
     3001 ttgtaaaccc tgtatttgcc aagtcattca gaatgagcag ccacatgcta aatgcccttt
     3061 atgcagaaat gatatacatg aagataattt attagaatgt cctccagaag aattagcacg
     3121 tgacagtgag aaaaagtctg atatggaatg gacatccagt tcaaagatta atgcgctaat
     3181 gcacgcattg actgacttaa gaaagaagaa tcccaacata aaaagtttgg ttgtttctca
     3241 gtttacaaca ttcctgtctt taatagaaat accacttaaa gcctctggat ttgtgtttac
     3301 tcgtttggat ggttccatgg cccaaaagaa aagagttgaa tcaattcagt gttttcaaaa
     3361 cactgaagca ggatctccaa ctataatgct tctgtcctta aaagcaggtg gagttggttt
     3421 gaatctgtct gcagcttctc gagtgttttt aatggatcca gcctggaatc ctgctgctga
     3481 agatcagtgc tttgacagat gccatagact tggtcagaag caagaagtta tcatcacaaa
     3541 attcattgta aaggactctg ttgaagaaaa tatgctgaaa atacaaaaca aaaagagaga
     3601 acttgcagca ggagcctttg gaactaaaaa accaaatgct gacgaaatga aacaagccaa
     3661 aattaatgaa atcagaacat taattgactt agagcggccg ctcgagtacc catacgacgt
     3721 acctgattat gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt
     3781 cccagactac gcatagtgag ggcccgatat ctcgacaatc aacctctgga ttacaaaatt
     3841 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3901 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3961 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4021 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4081 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4201 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     4261 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4321 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4381 atctcccttt gggccgcctc cccgcctgga aacgggggag gctaactgaa acacggaagg
     4441 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4501 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4561 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4621 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4681 atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4861 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4921 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg
     5101 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5401 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5461 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5521 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5581 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5641 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5701 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5761 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5821 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5881 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5941 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6001 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6061 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6121 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6181 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6241 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6301 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6361 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6421 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6481 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6661 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6901 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7021 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7261 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     7321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7501 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7921 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7981 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8041 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8101 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8161 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8221 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8281 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8341 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8401 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8461 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8521 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8581 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8641 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8701 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8761 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8821 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     8881 ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8941 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9001 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag g
//
