LOCUS       V026837                 6018 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026837
VERSION     V026837
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2165
                     /label="Gna12(NM_010302)"
                     /note="Gna12(NM_010302)"
                     /gene="Gna12"
     CDS             2184..2213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2232..2255
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2275..2291)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2302..2924
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3073..3661)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3990..4037
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4269..5063)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5098..5455)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5457..5561)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5588..6016
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtccggggtg gtgcggaccc ttagccgctg cttgctgccg gccgaggccg
     1081 gagcccgcga gcgcagggcg ggcgcggcgc gcgacgcgga gcgcgaggcc cgacggcgca
     1141 gccgcgacat cgacgcgctg ctggcccgcg agcggcgcgc ggtgcggcgg ctggtcaaga
     1201 tcctgctgct gggcgccggc gagagcggca agtccacctt cctcaagcag atgcgcatca
     1261 tccacggccg ggagttcgac cagaaggcgc tgctggagtt ccgcgacacc atcttcgaca
     1321 acatccttaa gggttcgagg gttcttgtgg acgctcgaga caagctcggc attccctggc
     1381 agcactctga gaacgagaag cacgggatgt ttctgatggc cttcgagaac aaggcagggc
     1441 tgcctgtgga gcctgccacc ttccagctct acgtgccagc cctgagtgcc ctctggagag
     1501 actcggggat cagggaagcc ttcagccgca gaagcgagtt ccagctgggt gaatcagtga
     1561 agtacttcct ggataacttg gaccggattg gccagctgaa ctacttcccc agtaagcaag
     1621 acatcctgct ggctagaaag gccaccaagg gaatcgtgga acatgacttc gttataaaga
     1681 aaatcccatt taagatggtg gatgtgggcg gccagaggtc acagcgccag aagtggttcc
     1741 agtgcttcga cggcatcaca tctatcctgt tcatggtgtc ctcgagcgag tatgaccagg
     1801 tcctcatgga ggacaggcgc accaaccggc tggtggagtc catgaacatc ttcgagacca
     1861 tcgtcaacaa caagctcttc ttcaacgtct ccatcatcct cttcctcaac aagatggacc
     1921 tcctggtgga gaaggtgaag tctgtgagca ttaagaagca cttcccagat ttcaagggcg
     1981 acccgcaccg gctggaggac gtccagcgct acctggtgca gtgcttcgac aggaagcgca
     2041 ggaaccgcag caagcccctg ttccaccact tcaccaccgc catagacacc gagaacatcc
     2101 gcttcgtgtt tcatgctgtg aaggacacga tcctgcagga gaacctgaaa gacatcatgc
     2161 tgcagacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     2221 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     2281 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     2341 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     2401 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     2461 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     2521 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     2581 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     2641 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     2701 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     2761 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     2821 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     2881 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     2941 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3001 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3061 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3121 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3181 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3241 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3301 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3361 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3421 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3481 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3541 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3601 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3661 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3721 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3781 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     3841 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     3901 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     3961 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     4021 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4081 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     4141 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     4201 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     4261 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4321 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4381 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     4441 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     4501 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     4561 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4621 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4681 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4741 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4801 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4861 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4921 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4981 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5041 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     5101 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     5161 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     5221 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     5281 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     5341 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     5401 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     5461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5581 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6001 aaatttaacg cgaatttt
//
