LOCUS       pEnCMV-Ext1(rat)-HA     8284 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027087
VERSION     V027087
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2943
                     /label="Ext1(NM_001130540)"
                     /note="Ext1(NM_001130540)"
                     /gene="Ext1"
     CDS             2950..2976
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2980..3006
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3010..3036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3060..3648
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3714..3762
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3964..4392
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4406..4735
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4802..5596
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5772..5893
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5942..5958)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5966..5982
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5990..6020)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6035..6056
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6344..6932)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7103..7963)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7964..8068)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgca ggccaaaaaa
      721 cgctatttca tcctgctctc agctggctct tgtctcgccc ttttgtttta ttttggaggc
      781 gtgcagttta gggcatcgag gagccacagc cggagagaag agcacagtgg tcggaatggc
      841 ttgcaccaac ccagtccgga tcatttctgg ccccgcttcc cggacgctct gcgccccttc
      901 tttccttggg atcaattgga aaacgaggat tccagcgtgc acatttcccc ccggcagaag
      961 cgggacgcca actcgagcat ctacaaaggc aagaagtgcc gcatggagtc ctgcttcgat
     1021 ttcacccttt gcaagaaaaa cggcttcaaa gtctacgtct acccgcagca gaaaggggag
     1081 aaaattgccg aaagttacca aaacattcta gcggccatcg agggctccag gttctacacc
     1141 tcggacccca gccaggcgtg cctctttgtc ctgagtctgg atactttaga cagagaccag
     1201 ttatcacctc agtatgtgca caatttgaga tccaaagtgc agagtctcca cttgtggaac
     1261 aatggtagga atcatttaat ttttaattta tattctggca cttggcctga ctacaccgag
     1321 gacgtggggt ttgacatcgg ccaggcgatg ctggccaaag ccagcatcag tactgaaaac
     1381 ttccgaccaa actttgatgt ttctattccc ctcttttcta aggatcatcc caggacagga
     1441 ggggagaggg ggtttttgaa atttaacacc atccctcccc tcaggaagta catgctggta
     1501 ttcaagggga agaggtacct gacagggata gggtcagaca ccaggaatgc cttatatcac
     1561 gtccataacg gggaggacgt cttgctcctc accacctgca agcatggcaa agactggcaa
     1621 aagcacaagg attctcgctg tgacagagac aacaccgagt atgagaaata tgattatcgg
     1681 gaaatgctgc acaatgccac tttctgtctg gttcctcgtg gtcgcaggct tgggtccttc
     1741 agattcctgg aggctttgca ggctgcctgt gtccctgtaa tgctcagcaa tggatgggag
     1801 ttgccattct ccgaagtgat aaattggaac caagctgccg tcataggcga tgagagattg
     1861 ctattacaga ttccttctac aatcaggtct attcatcagg ataaaatcct agcacttaga
     1921 cagcagacac agttcttgtg ggaggcttat ttttcttcag ttgagaagat tgtattaact
     1981 acactagaga ttattcagga cagaatattc aagcacatat cacgtaacag tttaatatgg
     2041 aacaaacatc ctggaggatt gttcgtccta ccacagtatt catcctacct gggagatttt
     2101 ccttactact atgctaattt aggtctaaag cccccctcca aattcactgc agtcatccat
     2161 gctgtgaccc ccctggtctc tcagtcccag ccagtgttga agcttcttgt ggcagcagcc
     2221 aaatcccagt actgtgcaca gattatagtt ctgtggaatt gtgacaagcc tctaccagcc
     2281 aaacaccgct ggcctgccac tgccgtgcct gtcatcgtca ttgaaggaga aagcaaggtt
     2341 atgagcagcc ggtttctgcc ctatgacaac atcatcactg atgctgtgct cagccttgat
     2401 gaggacactg tgctttcaac cacggaagtg gattttgcct tcactgtatg gcagagcttc
     2461 ccggagagga ttgtgggata tcctgcacgc agccatttct gggataactc aaaggagcga
     2521 tggggatata catccaagtg gacgaatgac tactccatgg tgttgacagg agctgctatc
     2581 taccacaaat attatcacta cctgtattcc cattacctgc cagccagcct gaagaacatg
     2641 gtagaccaac tggccaactg tgaggacatt ctcatgaatt tcctggtgtc tgctgtgacg
     2701 aaattgcctc caatcaaagt gacccagaag aaacagtata aggagacaat gatgggacag
     2761 acttcccgag catctcgctg ggctgaccct gaccactttg cccagcgaca gagctgcatg
     2821 aatacgtttg ccagctggtt tggctacatg ccgctgatcc actctcagat gaggctcgac
     2881 ccggtcctct ttaaagacca agtctcaatc ctgaggaaga aatacagaga cattgaacga
     2941 cttctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     3001 tatgctagct atccttatga tgtcccagac tacgcatagt gagggcccga tatctcgaca
     3061 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3121 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3181 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3241 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3301 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3361 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3421 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3481 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3541 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3601 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg
     3661 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     3721 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     3781 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     3841 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     3901 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     3961 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4021 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4081 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     4141 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4201 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4261 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4321 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4381 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4561 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4741 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     4801 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4861 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4921 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4981 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5041 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5101 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5161 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5221 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5281 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5341 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5401 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5461 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5521 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5581 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     5641 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5701 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5761 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5821 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5881 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5941 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6001 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6181 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6241 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     6541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6781 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7201 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8101 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
     8161 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8221 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8281 tagg
//
