LOCUS V041104 6877 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041104 VERSION V041104 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1533 /label="EXOSC8(NM_181503)" /note="EXOSC8(NM_181503)" /gene="EXOSC8" CDS 1540..1629 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 1653..2241 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2307..2355 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2557..2985 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2999..3328 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3395..4189 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4365..4486 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4535..4551) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4559..4575 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4583..4613) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4628..4649 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4937..5525) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5696..6556) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6557..6661) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggctgggttc 721 aaaaccgtgg aacctctgga gtattacagg agatttctga aagagaactg ccgtcctgat 781 ggaagagaac ttggtgaatt cagaaccaca actgtcaaca tcggttcaat tagtaccgca 841 gatggttctg ctttagtgaa gttgggaaat actacagtaa tctgtggagt taaagcagaa 901 tttgcagcac catcaacaga tgcccctgat aaaggatacg ttgttcctaa tgtggatcta 961 ccacccctgt gttcatcgag attccggtct ggacctcctg gagaagaggc ccaagtggct 1021 agccagttca ttgcagatgt cattgaaaat tcacagataa ttcagaaaga ggacttatgc 1081 atttctccag gaaagcttgt ctgggttcta tactgtgatc tcatttgcct cgactacgat 1141 ggaaacattt tggatgcctg cacatttgct ttgctagcgg ctttaaaaaa tgtacagttg 1201 cctgaagtta ctataaatga agaaactgct ttagcagaag ttaatttaaa gaagaaaagt 1261 tatttgaata ttagaactca tccagttgca acttcctttg ctgtgtttga tgacactttg 1321 cttatagttg accctactgg agaggaggaa catctggcaa caggaacctt aacaatagta 1381 atggatgagg aaggcaaact ctgttgtctt cacaaaccag gtggaagtgg gctaactgga 1441 gctaaacttc aggactgtat gagccgagca gttacaagac acaaagaagt taaaaaactg 1501 atggatgaag taattaagag tatgaaaccc aaactcgagg aacaaaaact aatatcagag 1561 gaagatttgg aacagaaact aatcagtgaa gaagatttag agcaaaagtt aatttccgag 1621 gaggacttat agtgagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg 1681 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 1741 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 1801 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 1861 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 1921 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 1981 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 2041 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 2101 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2161 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2221 ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac 2281 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg 2341 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 2401 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 2461 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 2521 cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 2581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 2641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 2701 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 2761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 2821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 2881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 2941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 3001 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3061 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 3121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 3181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 3241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 3301 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 3361 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 3421 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 3481 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 3541 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 3601 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 3661 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 3721 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 3781 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 3841 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 3901 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 3961 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 4021 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 4081 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 4141 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 4201 gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 4261 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 4321 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 4381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 4441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 4501 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 4561 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 4621 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4681 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4741 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4861 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4921 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4981 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5041 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5101 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 5161 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5221 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5281 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5341 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5401 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5461 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5521 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5581 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5641 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5701 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5761 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5821 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5881 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5941 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6001 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6061 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6121 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6181 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6241 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6301 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6361 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6421 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6481 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6541 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 6601 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6661 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 6721 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 6781 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 6841 aggcttgacc gacaattgca tgaagaatct gcttagg //