LOCUS       V033243                13013 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033243
VERSION     V033243
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             775..6939
                     /label="EVPL(NM_001320747)"
                     /note="EVPL(NM_001320747)"
                     /gene="EVPL"
     CDS             6955..7011
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             7012..7722
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     misc_feature    7789..8377
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8443..8491
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8693..9121
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9135..9464
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9531..10325
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10501..10622
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10671..10687)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10695..10711
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10719..10749)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10764..10785
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11073..11661)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11832..12692)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12693..12797)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccgc caccatgttc
      781 aaggggctga gcaaaggctc ccaggggaag gggtccccca agggctcccc cgccaagggg
      841 tcccccaaag gctcccccag caggcacagc cgggctgcca cccaggagct ggcccttctc
      901 atctcccgca tgcaagccaa cgccgaccag gtggagcggg acatcctgga gacgcagaag
      961 aggctgcagc aggaccggct gaacagtgag cagagccagg ccctgcagca ccagcaggag
     1021 acgggccgca gcctgaagga ggctgaggtg ctgctcaagg acctcttcct ggacgtggac
     1081 aaggcccggc ggctcaagca cccgcaggct gaggagattg agaaggacat caagcagctg
     1141 cacgagcggg tgacccagga gtgtgcggag taccgtgccc tgtacgagaa gatggtgctg
     1201 ccccccgacg tgggacccag ggtcgactgg gcacgcgtgc tggagcagaa acagaagcag
     1261 gtctgcgcag gccagtacgg gccgggcatg gcggagctgg agcaacagat cgccgagcac
     1321 aacatcctgc agaaggagat cgacgcctat gggcagcagc tgcggagcct cgtggggccg
     1381 gatgcagcca ccatccggag ccaataccga gacctactga aggcggcgtc gtggcgcggg
     1441 cagagcctgg gcagcctgta cacgcacctc cagggctgca cgcggcagct gagcgccctg
     1501 gctgagcagc agcgccgcat cctgcagcag gactggagcg acctcatggc cgaccctgcg
     1561 ggcgtgcggc gggagtacga gcacttcaag cagcacgagc tgctgagcca ggagcagagc
     1621 gtgaaccagc tggaggacga cggcgagcgc atggtggagc tgcggcaccc cgcggtgggg
     1681 cccatccagg cccaccagga ggccctgaag atggagtggc agaacttcct gaacctgtgt
     1741 atctgccagg agacccagct gcagcacgtg gaggactacc gccggttcca ggaagaggcc
     1801 gactcagtca gccagaccct ggcgaagctc aactccaact tggatgccaa gtacagccct
     1861 gcacctgggg gcccccctgg cgcccccaca gagctgctgc aacagctgga ggcagaggaa
     1921 aaacggctgg ccgtcaccga gagggccact ggggacctgc agcggcgaag ccgggatgtg
     1981 gcccctctgc cacagcgaag aaacccccct cagcagcccc tgcacgtgga cagcatctgc
     2041 gactgggact caggagaagt gcagctgctg cagggtgagc ggtataagct ggtagataac
     2101 actgacccgc acgcctgggt cgtgcagggc cctggcgggg agaccaagcg tgctcccgcc
     2161 gcctgcttct gcatcccagc accagaccct gatgctgtgg ccagggcctc ccggctggcc
     2221 tcagagctgc aggccctgaa gcagaaattg gccacagtcc agagccgcct gaaggccagt
     2281 gctgtggagt ctcttcggcc cagccagcag ggtacccagg gtggcccggg gagaggtggc
     2341 tgtggggcta gaaggagtag attccgactt ccgactgctc catctggctc agacctggcc
     2401 aacccacagg cccagaagct cctgacacag atgacccggc tggatggaga cctgggacag
     2461 atagagaggc aggtgctggc ctgggcgcgg gccccgctga gccgccccac acccttggag
     2521 gacttggagg gccgcatcca cagccatgag ggcacagccc agcgcctgca gagcctggga
     2581 acggagaagg agacagccca gaaggagtgc gaggcgtttc tgtccacgcg gcccgtgggc
     2641 cccgctgccc tgcagctgcc cgtagccctc aacagcgtga agaacaagtt cagtgacgtg
     2701 caggttctgt gcagcctcta cggggagaaa gccaaggctg ccctggatct ggagcggcag
     2761 atccaggatg cggacagggt catccgaggc ttcgaggcca ccctggtgca ggaggccccc
     2821 atccctgctg aaccgggggc tctgcaggag agggtcagcg agctgcagcg ccagcggagg
     2881 gagctgctgg aacagcagac ctgcgtgctg cggctacacc gcgcgctgaa ggcctcggag
     2941 cacgcatgcg ctgccctgca gaacaacttc caggagttct gccaagacct gcctcgccag
     3001 cagcgccagg tgcgagccct caccgaccgc taccacgccg taggggacca gctggacctg
     3061 cgggagaagg tggtgcagga tgccgccctc acctaccagc agttcaagaa ctgcaaggat
     3121 aacctgagct cctggctgga gcacctgccc cgcagccagg tgcggcccag cgacggcccc
     3181 agccagatcg cctacaagct gcaggcgcag aagaggctga cgcaggagat ccagagccga
     3241 gagcgggaca gggccacagc atcccacctc tcccaggccc tgcaggcagc gctccaggac
     3301 tatgagctcc aggcagacac ctaccgctgc tctttggagc ccaccctggc agtgtcagcc
     3361 cccaagagac cccgagtggc tcccctgcaa gagagcatcc aagcccagga gaagaacctt
     3421 gcaaaggcct atactgaggt tgcagcagca cagcagcagc tgctccagca gctggagttt
     3481 gctagaaaaa tgctggagaa gaaggagctc agtgaggaca tccgaaggac ccatgatgca
     3541 aagcagggct ccgagagccc tgcccaagca gggagagagt cagaggccct gaaggcccag
     3601 ctggaagagg agaggaagcg ggtggcccgg gtgcagcatg agctggaggc gcagaggagc
     3661 caactgctgc agctgaggac ccagcggccc ttggagaggc tggaggagaa ggaagtggta
     3721 gagttctacc gggaccccca gctggagggc agcctgtcca gggtgaaggc ccaggtggag
     3781 gaggagggca agcggcgggc tggcctgcag gcagacctgg aagtggcagc ccagaaggtc
     3841 gtgcagctgg aaagcaagag gaagaccatg cagcctcatc tgctgaccaa ggaggtcacc
     3901 caggtggaga gggaccccgg cctggacagc caggcggccc agctcaggat ccagatccag
     3961 cagctccgcg gggaggatgc cgtcatctcg gcccggctgg aagggctgaa gaaggagcta
     4021 ctggcccttg agaagaggga ggtggacgtg aaggagaagg tcgtggtgaa agaggtagtc
     4081 aaggtggaga agaatctgga aatggtcaag gcagcccagg ctctgaggct gcagatggag
     4141 gaggatgctg cgcggaggaa gcaggcggag gaggctgtgg ccaagctaca ggctcgcatc
     4201 gaagacctgg agcgggctat cagctcggtg gagcccaagg tcatcgtgaa ggaggtgaag
     4261 aaggtggagc aggacccagg gctcctccag gagtcctcca ggctgaggag cctcctcgag
     4321 gaggagagga ccaagaacgc gacgctggcc agggagctga gcgacctgca cagcaagtac
     4381 agcgtggtgg agaagcagag gcccaaagtg cagctccagg agcgcgtcca cgagatcttc
     4441 caggtggatc cggagacaga gcaggagatc actcggctca aggccaagct gcaggagatg
     4501 gcgggcaaga ggagcggtgt ggagaaggag gtggagaagc tgctgcccga cctggaggtc
     4561 ctgcgggccc agaagcccac ggtggagtac aaggaggtga cccaggaggt ggtgaggcat
     4621 gagaggagcc ccgaggtgct gcgtgagatc gaccgcctga aggctcagct caacgagctc
     4681 gtcaacagcc acgggcgctc ccaggagcag ctcatccgcc tgcagggtga gcgcgacgag
     4741 tggaggcgcg agcgggccaa ggtggagacc aagacggtga gcaaggaggt ggtgcgccac
     4801 gagaaggacc cggtgctgga gaaagaagca gagcggctcc gccaggaggt gcgggaggcg
     4861 gcccagaaga ggcgggccgc ggaggacgcg gtgtacgagc tgcagagcaa gcgcctgctg
     4921 ctggagagga ggaagcccga ggagaaggtg gtggtgcagg aggtggtggt cacccagaag
     4981 gacccgaagc tgcgcgagga gcacagccgg ctgagcggga gcctggatga ggaggtgggc
     5041 cggcggcgcc agctagagct tgaggtgcag cagctgcggg ccggcgtgga ggagcaggag
     5101 ggcctgctca gcttccagga ggaccgcagc aagaagctgg ccgtggagag ggagctgcgg
     5161 cagctgacct tgaggatcca ggagctcgag aagcggcctc ccacggtgca ggagaagatc
     5221 atcatggagg aagtggtcaa gctggagaag gacccggacc tggagaagtc cacggaagcc
     5281 ctgcggtggg acctggacca ggagaagacc caggtaaccg agctgaatcg ggagtgcaag
     5341 aacctgcagg tccagattga cgtcctccag aaagccaaat cgcaggagaa gaccatctac
     5401 aaggaagtga tccgggtgca gaaggaccgc gtcctggaag atgagcgggc ccgcgtgtgg
     5461 gagatgctca acagggagcg cacggcccgg caggcccggg aggaggaggc acggcgcctg
     5521 cgggagcgca ttgaccgggc cgagacgctg gggagaacct ggtcccggga ggagtccgag
     5581 ctgcagaggg cccgggacca ggccgaccag gagtgtgggc ggctgcagca ggagctgcgg
     5641 gctctggaga ggcagaagca gcagcagaca ctgcagctgc aggaggagtc gaagctgctc
     5701 agccagaaga cggagagcga gcgacagaag gcggcccagc ggggccagga gctctcgcgg
     5761 ctggaggcgg ccatcctccg cgagaaggac cagatctacg agaaggagcg gacgctccgg
     5821 gacctccacg ccaaggtgag ccgggaggag ctcagccagg agacccagac gcgagagacc
     5881 aacctttcca ccaagatctc catcctggaa cccgagacgg ggaaggacat gtccccatac
     5941 gaggcctaca agaggggcat catcgacagg ggccagtact tgcagctgca ggagctcgag
     6001 tgtgactggg aggaggtcac cacctcgggg ccctgtgggg aggagtctgt gctcctggac
     6061 cgcaagagcg ggaagcagta ctccatcgag gccgccctcc gctgccggcg catctctaag
     6121 gaggagtacc atctgtacaa ggacggccac ctgcccatct ccgagtttgc gctgcttgta
     6181 gctggggaga ccaagccaag ctcctcactc tccatcggct ctatcatctc caagtccccg
     6241 ctcgcctccc cggcccccca gagcaccagt ttcttctctc ccagcttctc tctcgggctc
     6301 ggtgatgaca gcttccctat cgccgggatc tatgacacaa ccacagacaa caagtgcagc
     6361 atcaagacgg ccgtggccaa gaacatgctg gaccccatca ctgggcagaa gctactggag
     6421 gcccaggcgg ccacaggggg catcgtggac ctgctcagcc gtgagcgcta ctctgtgcac
     6481 aaggcgatgg agaggggcct gatcgagaac acctccacac agaggctgct taacgcccag
     6541 aaggccttca ccggcatcga ggaccccgtc accaagaaga ggctctcggt gggcgaggcc
     6601 gtccagaagg gctggatgcc ccgggagagc gtgctcccac acctgcaggt gcagcacctg
     6661 accggggggc tcatcgaccc caagaggaca ggccgcatcc ccatccagca ggccctcctc
     6721 tccgggatga tcagtgaaga gctggcccag ctcctgcagg acgagtccag ctacgagaag
     6781 gatttgacag accccatctc caaggaacgg ctgagctaca aggaggccat gggccgctgc
     6841 cgcaaagacc ccctgagcgg cctgctgctc ctgccagcgg cactggaggg gtaccgctgc
     6901 taccgctccg cctcccccac cgtcccgcgc tcccttcgcg aattcggaag cggagctact
     6961 aacttcagcc tgctgaagca ggctggcgac gtggaggaga accctggacc tatggtgagc
     7021 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     7081 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7141 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     7201 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     7261 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7321 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7381 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     7441 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     7501 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     7561 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     7621 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     7681 ggccgccact ccaccggcgg catggacgag ctgtacaagt aactcgagtc tagagggccc
     7741 cttaagggtt cgatccctac cggttagtaa tgagtttgat atctcgacaa tcaacctctg
     7801 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7861 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7921 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7981 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8041 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8101 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8161 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8221 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8281 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8341 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg
     8401 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     8461 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     8521 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     8581 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     8641 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc
     8701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8821 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     8881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9061 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9121 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9181 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     9421 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     9481 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     9541 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     9601 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     9661 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     9721 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     9781 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     9841 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     9901 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     9961 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    10021 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    10081 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    10141 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    10201 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    10261 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    10321 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
    10381 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    10441 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10621 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    10681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    10741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    11221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
    11281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    11341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    11401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    11461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    11521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    11581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    11761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    12361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    12421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    12601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    12661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    12721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    12781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    12841 gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
    12901 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    12961 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
