LOCUS       V019728                13177 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019728
VERSION     V019728
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             652..669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             679..7920
                     /label="EP300(NM_001429)"
                     /note="EP300(NM_001429)"
                     /gene="EP300"
     misc_feature    7953..8541
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8607..8655
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8857..9285
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9299..9628
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9695..10489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10665..10786
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10835..10851)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10859..10875
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10883..10913)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10928..10949
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11237..11825)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11996..12856)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12857..12961)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgccaccat gcatcatcac
      661 catcaccatt taagcttgat ggccgagaat gtggtggaac cggggccgcc ttcagccaag
      721 cggcctaaac tctcatctcc ggccctctcg gcgtccgcca gcgatggcac agattttggc
      781 tctctatttg acttggagca cgacttacca gatgaattaa tcaactctac agaattggga
      841 ctaaccaatg gtggtgatat taatcagctt cagacaagtc ttggcatggt acaagatgca
      901 gcttctaaac ataaacagct gtcagaattg ctgcgatctg gtagttcccc taacctcaat
      961 atgggagttg gtggcccagg tcaagtcatg gccagccagg cccaacagag cagtcctgga
     1021 ttaggtttga taaatagcat ggtcaaaagc ccaatgacac aggcaggctt gacttctccc
     1081 aacatgggga tgggcactag tggaccaaat cagggtccta cgcagtcaac aggtatgatg
     1141 aacagtccag taaatcagcc tgccatggga atgaacacag ggatgaatgc gggcatgaat
     1201 cctggaatgt tggctgcagg caatggacaa gggataatgc ctaatcaagt catgaacggt
     1261 tcaattggag caggccgagg gcgacagaat atgcagtacc caaacccagg catgggaagt
     1321 gctggcaact tactgactga gcctcttcag cagggctctc cccagatggg aggacaaaca
     1381 ggattgagag gcccccagcc tcttaagatg ggaatgatga acaaccccaa tccttatggt
     1441 tcaccatata ctcagaatcc tggacagcag attggagcca gtggccttgg tctccagatt
     1501 cagacaaaaa ctgtactatc aaataactta tctccatttg ctatggacaa aaaggcagtt
     1561 cctggtggag gaatgcccaa catgggtcaa cagccagccc cgcaggtcca gcagccaggc
     1621 ctggtgactc cagttgccca agggatgggt tctggagcac atacagctga tccagagaag
     1681 cgcaagctca tccagcagca gcttgttctc cttttgcatg ctcacaagtg ccagcgccgg
     1741 gaacaggcca atggggaagt gaggcagtgc aaccttcccc actgtcgcac aatgaagaat
     1801 gtcctaaacc acatgacaca ctgccagtca ggcaagtctt gccaagtggc acactgtgca
     1861 tcttctcgac aaatcatttc acactggaag aattgtacaa gacatgattg tcctgtgtgt
     1921 ctccccctca aaaatgctgg tgataagaga aatcaacagc caattttgac tggagcaccc
     1981 gttggacttg gaaatcctag ctctctaggg gtgggtcaac agtctgcccc caacctaagc
     2041 actgttagtc agattgatcc cagctccata gaaagagcct atgcagctct tggactaccc
     2101 tatcaagtaa atcagatgcc gacacaaccc caggtgcaag caaagaacca gcagaatcag
     2161 cagcctgggc agtctcccca aggcatgcgg cccatgagca acatgagtgc tagtcctatg
     2221 ggagtaaatg gaggtgtagg agttcaaacg ccgagtcttc tttctgactc aatgttgcat
     2281 tcagccataa attctcaaaa cccaatgatg agtgaaaatg ccagtgtgcc ctccctgggt
     2341 cctatgccaa cagcagctca accatccact actggaattc ggaaacagtg gcacgaagat
     2401 attactcagg atcttcgaaa tcatcttgtt cacaaactcg tccaagccat atttcctacg
     2461 ccggatcctg ctgctttaaa agacagacgg atggaaaacc tagttgcata tgctcggaaa
     2521 gttgaagggg acatgtatga atctgcaaac aatcgagcgg aatactacca ccttctagct
     2581 gagaaaatct ataagatcca gaaagaacta gaagaaaaac gaaggaccag actacagaag
     2641 cagaacatgc taccaaatgc tgcaggcatg gttccagttt ccatgaatcc agggcctaac
     2701 atgggacagc cgcaaccagg aatgacttct aatggccctc tacctgaccc aagtatgatc
     2761 cgtggcagtg tgccaaacca gatgatgcct cgaataactc cacaatctgg tttgaatcaa
     2821 tttggccaga tgagcatggc ccagccccct attgtacccc ggcaaacccc tcctcttcag
     2881 caccatggac agttggctca acctggagct ctcaacccgc ctatgggcta tgggcctcgt
     2941 atgcaacagc cttccaacca gggccagttc cttcctcaga ctcagttccc atcacaggga
     3001 atgaatgtaa caaatatccc tttggctccg tccagcggtc aagctccagt gtctcaagca
     3061 caaatgtcta gttcttcctg cccggtgaac tctcctataa tgcctccagg gtctcagggg
     3121 agccacattc actgtcccca gcttcctcaa ccagctcttc atcagaattc accctcgcct
     3181 gtacctagtc gtacccccac ccctcaccat actcccccaa gcataggggc tcagcagcca
     3241 ccagcaacaa caattccagc ccctgttcct acacctcctg ccatgccacc tgggccacag
     3301 tcccaggctc tacatccccc tccaaggcag acacctacac caccaacaac acaacttccc
     3361 caacaagtgc agccttcact tcctgctgca ccttctgctg accagcccca gcagcagcct
     3421 cgctcacagc agagcacagc agcgtctgtt cctaccccaa cagcaccgct gcttcctccg
     3481 cagcctgcaa ctccactttc ccagccagct gtaagcattg aaggacaggt atcaaatcct
     3541 ccatctacta gtagcacaga agtgaattct caggccattg ctgagaagca gccttcccag
     3601 gaagtgaaga tggaggccaa aatggaagtg gatcaaccag aaccagcaga tactcagccg
     3661 gaggatattt cagagtctaa agtggaagac tgtaaaatgg aatctaccga aacagaagag
     3721 agaagcactg agttaaaaac tgaaataaaa gaggaggaag accagccaag tacttcagct
     3781 acccagtcat ctccggctcc aggacagtca aagaaaaaga ttttcaaacc agaagaacta
     3841 cgacaggcac tgatgccaac tttggaggca ctttaccgtc aggatccaga atcccttccc
     3901 tttcgtcaac ctgtggaccc tcagctttta ggaatccctg attactttga tattgtgaag
     3961 agccccatgg atctttctac cattaagagg aagttagaca ctggacagta tcaggagccc
     4021 tggcagtatg tcgatgatat ttggcttatg ttcaataatg cctggttata taaccggaaa
     4081 acatcacggg tatacaaata ctgctccaag ctctctgagg tctttgaaca agaaattgac
     4141 ccagtgatgc aaagccttgg atactgttgt ggcagaaagt tggagttctc tccacagaca
     4201 ctgtgttgct acggcaaaca gttgtgcaca atacctcgtg atgccactta ttacagttac
     4261 cagaacaggt atcatttctg tgagaagtgt ttcaatgaga tccaagggga gagcgtttct
     4321 ttgggggatg acccttccca gcctcaaact acaataaata aagaacaatt ttccaagaga
     4381 aaaaatgaca cactggatcc tgaactgttt gttgaatgta cagagtgcgg aagaaagatg
     4441 catcagatct gtgtccttca ccatgagatc atctggcctg ctggattcgt ctgtgatggc
     4501 tgtttaaaga aaagtgcacg aactaggaaa gaaaataagt tttctgctaa aaggttgcca
     4561 tctaccagac ttggcacctt tctagagaat cgtgtgaatg actttctgag gcgacagaat
     4621 caccctgagt caggagaggt cactgttaga gtagttcatg cttctgacaa aaccgtggaa
     4681 gtaaaaccag gcatgaaagc aaggtttgtg gacagtggag agatggcaga atcctttcca
     4741 taccgaacca aagccctctt tgcctttgaa gaaattgatg gtgttgacct gtgcttcttt
     4801 ggcatgcatg ttcaagagta tggctctgac tgccctccac ccaaccagag gagagtatac
     4861 atatcttacc tcgatagtgt tcatttcttc cgtcctaaat gcttgaggac tgcagtctat
     4921 catgaaatcc taattggata tttagaatat gtcaagaaat taggttacac aacagggcat
     4981 atttgggcat gtccaccaag tgagggagat gattatatct tccattgcca tcctcctgac
     5041 cagaagatac ccaagcccaa gcgactgcag gaatggtaca aaaaaatgct tgacaaggct
     5101 gtatcagagc gtattgtcca tgactacaag gatattttta aacaagctac tgaagataga
     5161 ttaacaagtg caaaggaatt gccttatttc gagggtgatt tctggcccaa tgttctggaa
     5221 gaaagcatta aggaactgga acaggaggaa gaagagagaa aacgagagga aaacaccagc
     5281 aatgaaagca cagatgtgac caagggagac agcaaaaatg ctaaaaagaa gaataataag
     5341 aaaaccagca aaaataagag cagcctgagt aggggcaaca agaagaaacc cgggatgccc
     5401 aatgtatcta acgacctctc acagaaacta tatgccacca tggagaagca taaagaggtc
     5461 ttctttgtga tccgcctcat tgctggccct gctgccaact ccctgcctcc cattgttgat
     5521 cctgatcctc tcatcccctg cgatctgatg gatggtcggg atgcgtttct cacgctggca
     5581 agggacaagc acctggagtt ctcttcactc cgaagagccc agtggtccac catgtgcatg
     5641 ctggtggagc tgcacacgca gagccaggac cgctttgtct acacctgcaa tgaatgcaag
     5701 caccatgtgg agacacgctg gcactgtact gtctgtgagg attatgactt gtgtatcacc
     5761 tgctataaca ctaaaaacca tgaccacaaa atggagaaac taggccttgg cttagatgat
     5821 gagagcaaca accagcaggc tgcagccacc cagagcccag gcgattctcg ccgcctgagt
     5881 atccagcgct gcatccagtc tctggtccat gcttgccagt gtcggaatgc caattgctca
     5941 ctgccatcct gccagaagat gaagcgggtt gtgcagcata ccaagggttg caaacggaaa
     6001 accaatggcg ggtgccccat ctgcaagcag ctcattgccc tctgctgcta ccatgccaag
     6061 cactgccagg agaacaaatg cccggtgccg ttctgcctaa acatcaagca gaagctccgg
     6121 cagcaacagc tgcagcaccg actacagcag gcccaaatgc ttcgcaggag gatggccagc
     6181 atgcagcgga ctggtgtggt tgggcagcaa cagggcctcc cttcccccac tcctgccact
     6241 ccaacgacac caactggcca acagccaacc accccgcaga cgccccagcc cacttctcag
     6301 cctcagccta cccctcccaa tagcatgcca ccctacttgc ccaggactca agctgctggc
     6361 cctgtgtccc agggtaaggc agcaggccag gtgacccctc caacccctcc tcagactgct
     6421 cagccacccc ttccagggcc cccacctgca gcagtggaaa tggcaatgca gattcagaga
     6481 gcagcggaga cgcagcgcca gatggcccac gtgcaaattt ttcaaaggcc aatccaacac
     6541 cagatgcccc cgatgactcc catggccccc atgggtatga acccacctcc catgaccaga
     6601 ggtcccagtg ggcatttgga gccagggatg ggaccgacag ggatgcagca acagccaccc
     6661 tggagccaag gaggattgcc tcagccccag caactacagt ctgggatgcc aaggccagcc
     6721 atgatgtcag tggcccagca tggtcaacct ttgaacatgg ctccacaacc aggattgggc
     6781 caggtaggta tcagcccact caaaccaggc actgtgtctc aacaagcctt acaaaacctt
     6841 ttgcggactc tcaggtctcc cagctctccc ctgcagcagc aacaggtgct tagtatcctt
     6901 cacgccaacc cccagctgtt ggctgcattc atcaagcagc gggctgccaa gtatgccaac
     6961 tctaatccac aacccatccc tgggcagcct ggcatgcccc aggggcagcc agggctacag
     7021 ccacctacca tgccaggtca gcagggggtc cactccaatc cagccatgca gaacatgaat
     7081 ccaatgcagg cgggcgttca gagggctggc ctgccccagc agcaaccaca gcagcaactc
     7141 cagccaccca tgggagggat gagcccccag gctcagcaga tgaacatgaa ccacaacacc
     7201 atgccttcac aattccgaga catcttgaga cgacagcaaa tgatgcaaca gcagcagcaa
     7261 cagggagcag ggccaggaat aggccctgga atggccaacc ataaccagtt ccagcaaccc
     7321 caaggagttg gctacccacc acagcagcag cagcggatgc agcatcacat gcaacagatg
     7381 caacaaggaa atatgggaca gataggccag cttccccagg ccttgggagc agaggcaggt
     7441 gccagtctac aggcctatca gcagcgactc cttcagcaac agatggggtc ccctgttcag
     7501 cccaacccca tgagccccca gcagcatatg ctcccaaatc aggcccagtc cccacaccta
     7561 caaggccagc agatccctaa ttctctctcc aatcaagtgc gctctcccca gcctgtccct
     7621 tctccacggc cacagtccca gcccccccac tccagtcctt ccccaaggat gcagcctcag
     7681 ccttctccac accacgtttc cccacagaca agttccccac atcctggact ggtagctgcc
     7741 caggccaacc ccatggaaca agggcatttt gccagcccgg accagaattc aatgctttct
     7801 cagcttgcta gcaatccagg catggcaaac ctccatggtg caagcgccac ggacctggga
     7861 ctcagcaccg ataactcaga cttgaattca aacctctcac agagtacact agacatacac
     7921 tagctcgagt ctagagggcc cgatatctcg acaatcaacc tctggattac aaaatttgtg
     7981 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8041 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8101 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8161 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8221 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8281 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8341 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8401 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8461 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8521 ccctttgggc cgcctccccg cctggaaacg ggggaggcta actgaaacac ggaaggagac
     8581 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg
     8641 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     8701 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     8761 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     8821 cagatctgcg cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     8881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9001 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     9121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     9181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct
     9241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     9301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9541 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     9601 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     9661 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     9721 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     9781 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     9841 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     9901 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     9961 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
    10021 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
    10081 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
    10141 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
    10201 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
    10261 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
    10321 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
    10381 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
    10441 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
    10501 gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
    10561 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    10621 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    10681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    10801 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10981 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11041 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11401 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
    11461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11641 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    11701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11881 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11941 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    12001 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    12061 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    12121 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    12181 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12241 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12301 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12361 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12421 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    12481 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    12541 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12601 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12661 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12721 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12781 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12841 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12901 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12961 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    13021 atggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    13081 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    13141 aggcttgacc gacaattgca tgaagaatct gcttagg
//
