LOCUS       V020974                10339 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020974
VERSION     V020974
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             649..1365
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1387..5016
                     /EC_number=""
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="precursor of human epidermal growth factor
                     receptor"
                     /protein_id=""
                     /transl_table=1
                     /translation="MRPSGTAGAALLALLAALCPASRA,LEEKKVCQGTSNKLTQLGTF
                     EDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLEN
                     LQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESI
                     QWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSG
                     RCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVN
                     PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNG
                     IGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTV
                     KEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEIS
                     DGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCW
                     GPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRG
                     PDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLE
                     GCPTNGPKIPS,IATGMVGALLLLLVVALGIGLFM,RRRHIVRKRTLRRLLQERELVEP
                     LTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREA
                     TSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGSLLDYVREHKDNI
                     GSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKE
                     YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSIL
                     EKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDER
                     MHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNS
                     TVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGS
                     VQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSH
                     QISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
                     /label="EGFR"
     CDS             5017..5082
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5115..5703
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5769..5817
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6019..6447
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6461..6790
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6857..7651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7827..7948
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7997..8013)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8021..8037
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8045..8075)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8090..8111
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9158..10018)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10019..10123)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgccaccat ggtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggagg agggggaagc
     1381 ggtaccatgc gaccctccgg gacggccggg gcagcgctcc tggcgctgct ggctgcgctc
     1441 tgcccggcga gtcgggctct ggaggaaaag aaagtttgcc aaggcacgag taacaagctc
     1501 acgcagttgg gcacttttga agatcatttt ctcagcctcc agaggatgtt caataactgt
     1561 gaggtggtcc ttgggaattt ggaaattacc tatgtgcaga ggaattatga tctttccttc
     1621 ttaaagacca tccaggaggt ggctggttat gtcctcattg ccctcaacac agtggagcga
     1681 attcctttgg aaaacctgca gatcatcaga ggaaatatgt actacgaaaa ttcctatgcc
     1741 ttagcagtct tatctaacta tgatgcaaat aaaaccggac tgaaggagct gcccatgaga
     1801 aatttacagg aaatcctgca tggcgccgtg cggttcagca acaaccctgc cctgtgcaac
     1861 gtggagagca tccagtggcg ggacatagtc agcagtgact ttctcagcaa catgtcgatg
     1921 gacttccaga accacctggg cagctgccaa aagtgtgatc caagctgtcc caatgggagc
     1981 tgctggggtg caggagagga gaactgccag aaactgacca aaatcatctg tgcccagcag
     2041 tgctccgggc gctgccgtgg caagtccccc agtgactgct gccacaacca gtgtgctgca
     2101 ggctgcacag gcccccggga gagcgactgc ctggtctgcc gcaaattccg agacgaagcc
     2161 acgtgcaagg acacctgccc cccactcatg ctctacaacc ccaccacgta ccagatggat
     2221 gtgaaccccg agggcaaata cagctttggt gccacctgcg tgaagaagtg tccccgtaat
     2281 tatgtggtga cagatcacgg ctcgtgcgtc cgagcctgtg gggccgacag ctatgagatg
     2341 gaggaagacg gcgtccgcaa gtgtaagaag tgcgaagggc cttgccgcaa agtgtgtaac
     2401 ggaataggta ttggtgaatt taaagactca ctctccataa atgctacgaa tattaaacac
     2461 ttcaaaaact gcacctccat cagtggcgat ctccacatcc tgccggtggc atttaggggt
     2521 gactccttca cacatactcc tcctctggat ccacaggaac tggatattct gaaaaccgta
     2581 aaggaaatca cagggttttt gctgattcag gcttggcctg aaaacaggac ggacctccat
     2641 gcctttgaga acctagaaat catacgcggc aggaccaagc aacatggtca gttttctctt
     2701 gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga gataagtgat
     2761 ggagatgtga taatttcagg aaacaaaaat ttgtgctatg caaatacaat aaactggaaa
     2821 aaactgtttg ggacctccgg tcagaaaacc aaaattataa gcaacagagg tgaaaacagc
     2881 tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc ccgagggctg ctggggcccg
     2941 gagcccaggg actgcgtctc ttgccggaat gtcagccgag gcagggaatg cgtggacaag
     3001 tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg catacagtgc
     3061 cacccagagt gcctgcctca ggccatgaac atcacctgca caggacgggg accagacaac
     3121 tgtatccagt gtgcccacta cattgacggc ccccactgcg tcaagacctg cccggcagga
     3181 gtcatgggag aaaacaacac cctggtctgg aagtacgcag acgccggcca tgtgtgccac
     3241 ctgtgccatc caaactgcac ctacggatgc actgggccag gtcttgaagg ctgtccaacg
     3301 aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct cttgctgctg
     3361 gtggtggccc tggggatcgg cctcttcatg cgaaggcgcc acatcgttcg gaagcgcacg
     3421 ctgcggaggc tgctgcagga gagggagctt gtggagcctc ttacacccag tggagaagct
     3481 cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg
     3541 ggctccggtg cgttcggcac ggtgtataag ggactctgga tcccagaagg tgagaaagtt
     3601 aaaattcccg tcgctatcaa ggaattaaga gaagcaacat ctccgaaagc caacaaggaa
     3661 atcctcgatg aagcctacgt gatggccagc gtggacaacc cccacgtgtg ccgcctgctg
     3721 ggcatctgcc tcacctccac cgtgcagctc atcatgcagc tcatgccctt cggctccctc
     3781 ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct caactggtgt
     3841 gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca ccgcgacctg
     3901 gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga ttttgggcgg
     3961 gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa agtgcctatc
     4021 aagtggatgg cattggaatc aattttacac agaatctata cccaccagag tgatgtctgg
     4081 agctacgggg tgactgtttg ggagttgatg acctttggat ccaagccata tgacggaatc
     4141 cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca gccacccata
     4201 tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc agatagtcgc
     4261 ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc ccagcgctac
     4321 cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc caacttctac
     4381 cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga gtacctcatc
     4441 ccacagcagg gcttcttcag cagcccctcc acgtcacgga ctcccctcct gagctctctg
     4501 agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct gcaaagctgt
     4561 cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg cgccttgact
     4621 gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca gtccgttccc
     4681 aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct gaaccccgcg
     4741 cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa ccccgagtat
     4801 ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc ccactgggcc
     4861 cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga cttctttccc
     4921 aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc agaataccta
     4981 agggtcgcgc cacaaagcag tgaatttatt ggagcagact acaaagacca tgacggtgat
     5041 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagctcga gtctagaggg
     5101 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     5521 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5701 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5761 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     5821 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     5881 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     5941 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg
     6001 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     6181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6361 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     6421 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6721 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6781 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     6841 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7021 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7381 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg
     7681 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7741 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7801 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7921 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10141 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac
    10201 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    10261 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10321 catgaagaat ctgcttagg
//
