LOCUS       V031181                 7503 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031181
VERSION     V031181
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3650
                     /label="Ddx54(NM_028041)"
                     /note="Ddx54(NM_028041)"
                     /gene="Ddx54"
     CDS             3669..3698
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3717..3740
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3760..3776)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3787..4409
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4558..5146)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5475..5522
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5754..6548)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6583..6940)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6942..7046)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7073..7501
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggctgctggc agacgtgtgg gacctggccc gccgtcgcgt cccaccatgg
     1081 cgccgtggaa gaagaagagg ctgcggaaac gccgaactgg ggcttcccaa ggccgcgaca
     1141 gcgactcgga tgacggcgag ttcgagatcc aggcggagga tgacgcccgg gcgaggaagc
     1201 tgggccctgg cagagccttg ccctcatttc ctacctcaga gtgcgtatca gatgtggagc
     1261 ccgacactcg ggagatggtg cgagcccaga acaagaaaaa gaagaagtct ggaggcttcc
     1321 agtccatggg cctgagttac cctgtgttca aggggatcat gaaaaagggc tacaaggtgc
     1381 cgacgcccat ccagaggaag accatccccg tgatcttgga tggcaaggat gtggtggcca
     1441 tggcccggac aggcagtggc aagacggcct gcttcctcct cccgatgttt gagcggctga
     1501 aggcacgcag tgcacagacg ggggctcgag ccctcatcct ctcacccacc cgggagctgg
     1561 ccctgcagac catgaagttc actaaagagc taggcaagtt caccggcctc aagactgcct
     1621 tgatcctggg tggagacaaa atggaagacc agtttgcagc cctgcacgag aaccctgaca
     1681 taatcattgc cacccctggg cgtctggtgc atgtggctgt ggagatgaac ttgaagctgc
     1741 agagtgtgga gtatgtggtg ttcgatgaag cagacaggct ctttgaaatg ggctttgctg
     1801 agcagctaca ggagatcata ggccgccttc ctgggggcca ccagacggtg ctgttctcag
     1861 ctacactgcc caagctgctg gtggaatttg cacgggcagg cctcacagag cccgtgctca
     1921 tccgcctgga cgtagactcc aagctcaatg agcagctcaa gacctccttc ctccttgtgc
     1981 gcgaagacac caaggctgct gtgctcctct acctgctgca gaatgtcgtt cggccccagg
     2041 accagactgt ggtgtttgta gccacaaagc accatgcgga gtacctcaca gagttgctga
     2101 tgggccaggg tgtgagttgc gcccacatct atagtgcctt ggaccagacg gcccgcaaga
     2161 tcaacttggc caagttcaca cacaacaaat gttccaccct catcgtgact gacctggccg
     2221 cccggggcct ggacatccca ctgctggaca acgtcatcaa ctacagcttc cctgccaagg
     2281 gcaagctctt cctgcaccga gtgggccgtg tggcccgagc aggccgaagt ggcacagcct
     2341 attctttggt ggccccagac gaggtcccct acctgcttga cctacacctg ttcctgggcc
     2401 gctctgtcac cctggcccgt ccttgtgagg agccttcagt ggcagatgcg gttggcaggg
     2461 acggagtgct gggtcgcgtg ccccagagtg tagtggatga tgaggacagc agcctgcaga
     2521 ctgccatggg ggcatccctg gatcttcagg gcctgcaccg cgtggccaac aacgctcagc
     2581 agcagtatgt gcgctcacgg ccagcgccct cgcctgagtc catcaagaga gccaaggagc
     2641 tggacctggc agagctgggc ttgcacccac tcttcagctc atgctttgag gagggagagc
     2701 tccaacgcct gaggctggtg gacagcatca agaactatcg cacgcgcaca accatctttg
     2761 agatcaatgc ctccagcaag gacccaagca gccaaatgat gcgtgccaag cggcagaggg
     2821 accggaaagc tgttgccagt ttccagcagc ggcgccagga aaggcaggaa ggcccagctg
     2881 acccagcccc ccagagggag ctgcctcagg aggaggagga ggagatggtt gagactgtag
     2941 agggtgtctt cacagaagtc gtgggccaga aacggccaag gccgggaccc agccaaggag
     3001 ccaagaggcg gaggatggag acccgtcagc gagaccagga gttttatgtc ccctaccggc
     3061 ccaaggattt cgacagtgag cgggggctga gtgtcagtgg ggctggaggg gcctttgagc
     3121 agcaggtggc tggtgcagtc ctggacctga tgggggatga agcacagaac atgagccggg
     3181 ggcagcagca gctcaagtgg gaccggaaga agaagcggtt tgtggggcag tcaggccaag
     3241 aagacaagaa aaagatcaag acagagagcg gccggtttat cagcagctct tacaagcggg
     3301 atctctacca gaagtggaag cagaagcaga aaattgatga ccgggactcc gaggaagaag
     3361 ggccatccaa ccagcgaggc cctgggcccc gcagaggtgg aaagcgaggt cgtagtcaag
     3421 gcacatccca gccccgagct tccagtgtac ccgcaggccg catgcgctcg gaactcaaga
     3481 ccaaggagca aatcctcaag cagcgccggc aagctcagaa gcagcgcttc ctgcagcgag
     3541 ggggcctgaa gcagctttca gcacgcaacc gacgccgagc ccaggagctg cgccagggcg
     3601 cctttggccg gggtgctccc tccaggaagg gcaagatgag gaaaaggatg acgcgtacgc
     3661 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     3721 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     3781 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     3841 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     3901 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     3961 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     4021 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     4081 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     4141 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     4201 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     4261 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     4321 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     4381 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     4441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5281 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     5341 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     5401 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     5461 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     5521 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     5581 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     5641 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     5701 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     5761 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     5821 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     5881 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     5941 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6001 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6061 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6121 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     6181 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     6241 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     6301 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     6361 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     6421 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     6481 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     6541 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     6601 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     6661 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     6721 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     6781 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     6841 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     6901 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     6961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     7081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7501 ttt
//
