LOCUS       V029571                 7101 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029571
VERSION     V029571
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3248
                     /label="Dclk1(NM_001195538)"
                     /note="Dclk1(NM_001195538)"
                     /gene="Dclk1"
     CDS             3267..3296
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3315..3338
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3358..3374)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3385..4007
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4156..4744)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5073..5120
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5352..6146)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6181..6538)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6540..6644)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6671..7099
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtcgttcggc agagatatgg agttggagca ttttgatgag cgggacaagg
     1081 cgcagaggta cagcaggggg tcccgtgtga atggcctgcc cagccccaca cacagcgccc
     1141 actgcagctt ctaccgcacc cgcaccctgc agacactcag ctccgagaag aaagccaaga
     1201 aggttcgatt ctacagaaat ggtgaccgct acttcaaagg aattgtgtat gccatctccc
     1261 cagaccgctt cagatctttc gaggccctgc tggctgattt gacccgaact ctctcggata
     1321 atgtgaattt gccccagggg gtgagaacca tctacaccat cgatggactc aagaagatct
     1381 ccagcctgga ccagctggtg gaaggtgaaa gctatgtctg cggctccatc gagcccttta
     1441 agaagctgga gtacaccaag aatgtgaacc ccaactggtc agtgaacgtc aagaccacct
     1501 cagcctcccg cgcagtgtct tctttggcca ctgccaaggg tgggccttcg gaggttcggg
     1561 agaataagga tttcattcga cccaagctgg tcaccatcat cagaagtggg gtgaagccac
     1621 ggaaggctgt cagaatcctg ctgaacaaga agacggctca ctccttcgag caggttctca
     1681 ctgacattac cgacgctatc aagctggact ccggtgtggt gaagcgcctg tacactctgg
     1741 atgggaagca ggtgatgtgc cttcaggact tttttggtga cgatgacatt tttattgcat
     1801 gtggaccaga gaagttccgt taccaggatg atttcttgct agatgaaagt gaatgtcgag
     1861 tggtgaaatc aacttcttac accaaaatag catcagcgtc ccgcagaggc acaaccaaga
     1921 gcccaggacc ttcccggaga agcaagtccc cagcctccac cagctcagtt aatggaaccc
     1981 ctggtagtca gctctctact ccacgctcgg gcaagtcacc aagtccatca cccaccagcc
     2041 caggaagcct gcggaagcag aggatctctc agcatggcgg ctcctcgact tcactttcat
     2101 ccactaaagt ttgcagctca atggatgaga atgatggccc tggggaagaa gagtctgagg
     2161 aaggcttcca gattcctgcc acaataacag agagatacaa agtcgggaga acaataggag
     2221 acggaaattt tgctgttgtc aaggaatgta tagagaggtc gactgctcgg gagtatgccc
     2281 tgaaaatcat caagaaaagc aaatgccgag gcaaagagca catgatccag aacgaggtct
     2341 ccatcctacg gagggtgaag caccccaaca ttgtcctcct gattgaagag atggatgtgc
     2401 cgactgaact gtatcttgta atggaattag tgaagggtgg agaccttttc gatgccatca
     2461 cctccactag caaatacaca gagagagatg ccagcgggat gctgtacaac ctggccagcg
     2521 ccatcaaata cctgcacagc ctgaacatcg tccaccgtga catcaagcca gagaatctgc
     2581 tggtgtatga gcaccaggat ggcagtaagt cactcaagtt gggtgacttt ggcctggcca
     2641 caattgtcga cggccccctg tacacagtct gtggcacccc aacatatgtg gctccagaaa
     2701 tcattgcaga gactggatat ggcctcaagg tggacatctg ggcagctggc gtgatcactt
     2761 atatcctgct gtgtggcttc cctccgttcc gtggaagtgg ggatgaccag gaggtgcttt
     2821 ttgaccagat cttgatgggc caagtggact ttccatctcc gtattgggac aatgtgtcag
     2881 attctgctaa ggagctcatc aacatgatgc tgttggttaa cgtggaccag agattttcag
     2941 ccgtgcaggt ccttgagcat ccctgggtta atgatgatgg tctcccagaa aatgagcatc
     3001 agctgtcagt agctggcaaa atcaagaagc atttcaacac aggccccaag ccgagcagca
     3061 ctgcagcagg agtttctgta atagcaacca ccgctcttga taaggagagg caggttttcc
     3121 gacgaagacg caaccaggat gtgaggagcc ggtacaaggc gcagccagct ccaccggaat
     3181 tgaactcgga atcggaggac tactccccca gctcctctga gactgttcgc tcccccaatt
     3241 cgccctttac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
     3301 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
     3361 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     3421 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     3481 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     3541 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     3601 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     3661 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     3721 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     3781 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     3841 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     3901 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     3961 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     4021 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc
     4921 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     4981 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     5041 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     5101 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     5161 ttcagttagc ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     5221 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     5281 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     5341 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     5401 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     5461 gcaatatcac gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca
     5521 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     5581 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     5641 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     5701 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     5761 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     5821 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     5881 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     5941 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     6001 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     6061 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     6121 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc
     6181 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6241 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6301 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6361 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6421 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     6481 tggggacttt ccacacccta actgacacac attccacagc tggttctttc cgcctcagga
     6541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6661 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6721 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6781 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6841 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6901 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6961 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7021 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7081 caaaaattta acgcgaattt t
//
