LOCUS       V031068                 5418 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031068
VERSION     V031068
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1565
                     /label="Dcdc2c(NM_001177964)"
                     /note="Dcdc2c(NM_001177964)"
                     /gene="Dcdc2c"
     CDS             1584..1613
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1632..1655
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1675..1691)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1702..2324
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2473..3061)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3390..3437
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3669..4463)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4498..4855)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4857..4961)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4988..5416
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gattggagaa aaagtcttcc ctctaggagg agttcgaaag ttgtttacaa
     1081 tggatggaca tcttttggat gactcaaaga atttgcaaga caattatttt tatgttgctg
     1141 caggactgga gacttttaaa tccattcctt actggaagtc ctcatgggtc cccagtgagg
     1201 tccagcagag atttggaggc aatgataaat acacgcaaac aaagaaaaga gtggagtcca
     1261 aagtaaaaga acctcttcaa aatgacagtg taccacctag aagtcaggat tctgtttatt
     1321 atgccaaaga gaaaaagcag atggatacag aacttttagt tcaaagtggt gcagaaggtg
     1381 atgtttataa agcccagact ccggctaagg aggcccaaga agccctggaa gtcaaggagg
     1441 acccagaggt gaaggtggag gtgccagtcg atcaggcacc agctgaaatt gtcaaagaaa
     1501 ttgacgagat aggcgatagc agccctggcc tgaagagtgg catgcatatt cctgcttcat
     1561 tcatgacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     1621 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     1681 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     1741 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     1801 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     1861 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     1921 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     1981 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     2041 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     2101 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     2161 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     2221 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     2281 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     2341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3181 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     3241 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     3301 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     3361 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     3421 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     3481 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     3541 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     3601 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     3661 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     3721 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     3781 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     3841 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     3901 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     3961 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4021 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4081 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4141 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4201 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4261 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4321 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4381 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4441 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     4501 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4561 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4621 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4681 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     4741 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4801 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     4861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4981 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5401 aaatttaacg cgaatttt
//
