LOCUS       V030850                 6747 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030850
VERSION     V030850
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2894
                     /label="Dbh(NM_138942)"
                     /note="Dbh(NM_138942)"
                     /gene="Dbh"
     CDS             2913..2942
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2961..2984
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3004..3020)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3031..3653
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3802..4390)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4719..4766
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4998..5792)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5827..6184)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6186..6290)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6317..6745
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gcaagctcat ctcagccacc agccttgttg gagcagcctc ccaagcccca
     1081 gtgtccgtga ggcggcttcc atgtacggca ctgctgtggc catcttcctg gtcatcctgg
     1141 tggctgcact gcggggctcg gagcctccag agagcccctt cccctaccac atccccctgg
     1201 accccgaagg gattttagag ctctcatgga acgtcagcta tgtccaggag atcatccact
     1261 tccagctcca ggtgcaaggg ctgagggctg gggtcctgtt tggaatgtca gatcgaggag
     1321 agatggagaa cgcagatctc atcatgctct ggactgatgg ggacagggcc tactttgcgg
     1381 atgcctggag tgaccggaaa gggcagatcc atctggattc ccagcaagac taccagctgc
     1441 tccaggcaca gaggactcga gatggcctgt ccctgctctt caagaggccc tttgtcacct
     1501 gtgaccccaa ggattatgtc attgaggatg acactgtcca tctagtgtat gggatcttgg
     1561 aagagccatt tcagtcgctg gaggccatca acacctcagg cctgcataca gggctgcagc
     1621 gggtgcagct tctgaagtct gaggtcccca ctccatccat gcctgaggat gtacaaacca
     1681 tggatatccg ggctcctgac atcctcatcc ccgacaatga gaccacgtac tggtgctata
     1741 tcactgagct acccccacgc ttcccccgac accacatcat catgtatgag gccattgtca
     1801 ctgagggcaa tgaggccctg gtgcaccaca tggaggtctt ccaatgtgca gctgagtctg
     1861 aggacttccc ccagttcaac ggaccctgtg actccaaaat gaagcctgac cgactcaact
     1921 actgccggca cgtgctggca gcatgggccc tgggtgccaa ggcattttac tacccaaaag
     1981 aagctggtgt tccctttggg ggcccggggt cctccccgtt tctccgactg gaagttcatt
     2041 accacaaccc acggaagata caaggccggc aggactcctc tggcatccgc ctacactaca
     2101 cagctactct ccgacgctat gatgcgggca tcatggagct cggactggtg tatacaccct
     2161 tgatggccat cccccctcag gagactgcct ttgtgttgac cggctactgc acagacaagt
     2221 gcacccagat ggcactgcag gactccggaa tccacatctt tgcctctcag cttcatacac
     2281 acctgacggg caggaaggtg gttactgtgc tcgccaggga tggccaagag aggaaggtgg
     2341 tgaacaggga caaccactac agcccccact tccaggagat cagaatgctg aagaaggttg
     2401 tgacagtcta cccgggggac gtactcatca cttcatgcac atacaacaca gaaaacaaga
     2461 cgctggccac agtggggggg tttggaatct tggaggagat gtgtgtcaac tatgtgcact
     2521 actaccccca gacagagctg gaactctgca agagtgccgt ggacgacggc ttcctacaga
     2581 aatacttcca catggtaaac aggttcagca gtgaggaggt ctgcacctgc cctcaggcct
     2641 cagtccccca gcagttctcc tccgtgccct ggaactcttt caatcgggat atgctcaagg
     2701 ctctgtatga ctacgcccct atctccatgc attgcaacaa gacttctgct gtccgcttcc
     2761 cgggtgagtg gaatctgcag cctttgccta agatcacctc cacgctggaa gaacccaccc
     2821 cacgctgccc catccgacag actcagagcc ccgctaaccc cactgtgccc atcacaacag
     2881 aggcagatgc tgaaacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc
     2941 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc
     3001 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg
     3061 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     3121 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     3181 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     3241 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     3301 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     3361 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     3421 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     3481 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     3541 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     3601 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg
     3661 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     4561 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     4621 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     4681 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     4741 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     4801 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     4861 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     4921 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     4981 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     5041 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     5101 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc
     5161 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     5221 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     5281 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     5341 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     5401 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     5461 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     5521 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     5581 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     5641 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     5701 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     5761 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     5821 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     5881 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     5941 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     6001 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     6061 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6121 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     6181 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6241 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6301 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6361 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6421 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6481 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6541 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6601 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6661 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6721 atttaacaaa aatttaacgc gaatttt
//
