LOCUS V018600 7880 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018600 VERSION V018600 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 671..2527 /label="DTX4(NM_015177)" /note="DTX4(NM_015177)" /gene="DTX4" CDS 2567..2632 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2656..3244 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3310..3358 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3560..3988 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4002..4331 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4398..5192 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5368..5489 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5538..5554) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5562..5578 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5586..5616) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5631..5652 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5940..6528) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6699..7559) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7560..7664) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgccacc atgctcctgg cctcggccgt ggtggtctgg gaatggctga acgagcacgg 721 ccgctggcgt ccctacagcc cagcggtgag ccaccacatc gaggcggtgg tccgcgccgg 781 cccccgcgcg gggggcagcg tggtgctggg ccaggtggac agccgtctcg cgccctacat 841 catcgacctg cagtccatga accagttccg ccaagacacg ggaactctcc gcccagttcg 901 ccgcaactac tacgacccct cctcggcccc tgggaagggc gtggtgtggg agtgggagaa 961 cgacaatggc tcctggacgc cctacgacat ggaagtgggc atcaccatcc agcatgccta 1021 tgagaagcag cacccctgga tcgacctcac ttccattggc tttagctacg taattgactt 1081 caacaccatg ggccagatca accgtcagac ccagcgccaa cgccgcgtcc gccggcgcct 1141 cgacctcatc taccccatgg tcacagggac cttgcctaag gctcagtcct ggccagtcag 1201 ccctgggcca gccacctcgc cccccatgtc cccctgctcc tgtccccagt gtgtcttggt 1261 gatgagtgtt aaggcagccg tggtcaatgg cagcactggg cccctacagc tgccagtgac 1321 ccgcaagaac atgccgcctc ctggagtggt caagctaccc ccactgccag gctctggggc 1381 caagccactg gacagcacag gcaccattcg aggcccactg aagaccgccc catcgcaggt 1441 gatccggaga caagcctcca gcatgcccac tgggacaacc atgggctctc ctgccagtcc 1501 cccaggaccc aacagcaaga ccggaagggt ggccctggcc accttgaatc gtaccaacct 1561 gcagcgactg gccattgccc agtcccgggt gctgatcgcc tctggggtcc ccacagtccc 1621 agtgaagaac ctaaatgggt ccagtcctgt caaccctgcc ttggcaggaa tcactgggat 1681 cctcatgagt gcagcggggc tgcctgtgtg tctcaccagg ccaccaaagc tggtcctaca 1741 cccacccccc gtcagcaaga gtgaaataaa atccatccca ggggtttcca acacaagccg 1801 caagaccacc aaaaaacaag ccaagaaagg taaaacccca gaggaagtgc taaaaaaata 1861 tctacagaaa gtccggcacc caccagatga ggactgcacc atctgtatgg aacgcctcac 1921 ggccccctca ggctacaagg gcccgcagcc tacggtaaaa cctgacctgg tagggaagct 1981 gtccagatgc ggccacgtct accacatcta ctgcttggtt gccatgtaca acaatgggaa 2041 caaggatgga agtttgcagt gtccaacctg caagaccatt tatggggtga agacaggcac 2101 ccaacctcca gggaagatgg agtaccacct catcccccac tccttgcctg gccacccaga 2161 ctgcaaaacc atccggatca tctacagcat cccccccggc attcagggac cggaacaccc 2221 gaatcctggg aagagtttca gcgcccgagg cttcccacga cactgttacc ttccggacag 2281 cgagaaaggg agaaaagttc tgaagctgct gctcgtggcc tgggatcgcc gcctcatttt 2341 tgccattggc acctccagca ccacaggcga gtcagacacc gtcatctgga atgaggtcca 2401 ccacaagaca gagtttggct ctaatctcac tggccatggc tacccagatg ccaattacct 2461 ggataatgtg ctggctgaac tggctgccca gggcatctct gaggacagca ctgcccagga 2521 gaaggacgaa ttctgcagat atccagcaca gtggcggccg ctcgaggact acaaagacca 2581 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgagg 2641 gcccgatatc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 2701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 2761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 2821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 2881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 2941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 3001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 3061 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 3121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 3181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 3241 ccgcctggaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 3301 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat 3361 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 3421 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 3481 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg 3541 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 3601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 3661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc 3721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 3781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 3841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 3901 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag 3961 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 4021 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4081 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4141 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4261 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4321 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 4381 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 4441 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 4501 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 4561 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 4621 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 4681 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 4741 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 4801 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 4861 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 4921 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 4981 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 5041 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 5101 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 5161 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc 5221 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 5281 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 5341 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 5401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5461 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 5521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 5761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6121 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6361 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 6421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 7021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 7081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 7141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 7201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 7681 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta 7741 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 7801 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 7861 gcatgaagaa tctgcttagg //