LOCUS       V031288                 5976 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031288
VERSION     V031288
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2123
                     /label="Cxadr(NM_001025192)"
                     /note="Cxadr(NM_001025192)"
                     /gene="Cxadr"
     CDS             2142..2171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2190..2213
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2233..2249)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2260..2882
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3031..3619)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3948..3995
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4227..5021)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5056..5413)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5415..5519)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5546..5974
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcgcgccta ctgtgcttcg tgctcttgtg cgggatcgcg gatttcacca
     1081 gtggtttgag catcactaca cccgaacaga ggatcgaaaa agccaaaggg gaaactgcgt
     1141 atctaccatg caagtttact ctcagtcccg aagaccaggg accactggac attgaatggc
     1201 tgatatcccc gtctgataac cagatagtgg atcaagtgat cattttgtat tctggagaca
     1261 aaatttatga taactactat ccggatctga aaggacgggt acattttacg agtaacgatg
     1321 tcaagtctgg cgacgcatct ataaatgtga ccaacctgca gctgtcggac attggcactt
     1381 accagtgcaa agtgaagaaa gcccctgggg ttgcaaataa gaaattcctg ctgaccgttc
     1441 ttgttaagcc ttcaggtaca agatgcttcg tggatggatc ggaagagatt ggaaatgact
     1501 tcaagctaaa atgtgaaccc aaggaaggct cccttccact acagtttgaa tggcagaaac
     1561 tgtcggactc ccagacaatg cctacgccat ggctggcaga aatgacgtca ccagttatat
     1621 ctgtgaagaa cgccagttct gagtattctg ggacatacag ctgcacggtt caaaacagag
     1681 tgggctctga ccagtgtatg ctgcgactag acgttgtccc accctccaac cgagccggaa
     1741 cgatcgcggg cgccgtcata gggacgctgc tggcccttgt gctcatcggg gccatcctct
     1801 tctgctgtca caggaaacgc agagaagaga agtacgagaa ggaagttcat catgatatca
     1861 gggaagatgt gcctcctcca aagagtcgga catccactgc caggagctat attggcagca
     1921 accattcctc cctgggatcc atgtccccct ctaacatgga ggggtattcc aagacgcagt
     1981 ataaccaagt ccccagtgaa gactttgaac gtgcgcctca gagcccgact ctggcacccg
     2041 ctaaggtagc tgcccctaat ctcagtcgaa tgggagcggt tcctgtgatg attcctgcac
     2101 agagcaagga cgggtctata gtaacgcgta cgcggccgct cgagcagaaa ctcatctcag
     2161 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     2221 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     2281 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2341 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2401 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2461 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     2521 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     2581 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     2641 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     2701 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     2761 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     2821 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     2881 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2941 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     3781 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     3841 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     3901 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     3961 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4021 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4081 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4141 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4201 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4261 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4321 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4381 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4441 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4501 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4561 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4621 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4681 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4741 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4801 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     4861 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4921 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4981 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5041 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5101 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5161 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5221 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5281 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5341 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5401 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5521 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5581 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5641 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5701 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5761 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5821 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5881 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5941 aatgagctga tttaacaaaa atttaacgcg aatttt
//
