LOCUS V030072 7569 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030072 VERSION V030072 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..3716 /label="Csf2rb(NM_007780)" /note="Csf2rb(NM_007780)" /gene="Csf2rb" CDS 3735..3764 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 3783..3806 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(3826..3842) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 3853..4475 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(4624..5212) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 5541..5588 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(5820..6614) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(6649..7006) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(7008..7112) /gene="bla" /label="AmpR promoter" rep_origin 7139..7567 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggaccagcaa atggcactca catgggggct gtgctacatg gcactggtgg 1081 ctctctgttg gggacacggg gtgacagagg cagaagaaac ggtccctctg aagactctgc 1141 agtgctacaa tgactacacc aaccacatca tctgcagctg ggcggacaca gaggatgccc 1201 aggggctaat caacatgacc ctctatcacc agctagagaa aaaacagcca gtgtcctgtg 1261 agctcagtga ggaactcatg tggtcagagt gcccgtcatc ccaccgctgt gtgcccagaa 1321 gatgtgtcat cccctataca cgattttcca tcacaaacga agactactac tccttccggc 1381 cagatagtga tctgggcatc cagctcatgg tgccacttgc ccagaatgtg cagccaccac 1441 ttcccaagaa cgtcagcatc agctcctctg aggatcgttt cctgctggag tggagtgtgt 1501 cccttgggga tgcccaggtc tcctggcttt catcaaagga catagagttt gaggtggctt 1561 ataagcggct tcaggactcc tgggaggatg cctacagtct ccacactagc aaatttcagg 1621 tgaatttcga gccaaagcta ttcctaccca acagcatcta tgctgcccgt gtgcgcactc 1681 ggctgtcccc gggttcaagc ttgtctggga gacccagcag atggagccca gaggttcact 1741 gggactccca gccaggggac aaggcccagc cacagaacct tcaatgcttc tttgatggga 1801 tccagtccct ccactgctcc tgggaggtgt ggacccagac gactggctct gtttcctttg 1861 ggctcttcta tcgccccagc cctgtagctc cggaggagaa atgctctccg gtggtgaagg 1921 agccgccggg ggccagtgtc tacacccgct accattgcag tctacctgtg cctgagccca 1981 gtgcacacag ccagtacaca gtctctgtta agcacctgga acaagggaag ttcatcatga 2041 gctataacca catccagatg gagcctccaa ccctcaacct gaccaagaac agagacagct 2101 acagcctgca ttgggaaact cagaagatgg cttactcatt cattgagcac acattccagg 2161 tccagtacaa gaagaaatcg gacagctggg aggacagcaa gacagagaac ctagatcgag 2221 cccatagcat ggacctctcc cagctggagc cagacacctc atactgcgcc agggtgaggg 2281 tcaagcccat ctctaactac gatgggatct ggagcaagtg gagcgaagag tacacttgga 2341 agactgactg ggtgatgccc acgctgtgga tagtcctcat cctggtcttt ctcatcctca 2401 ccttgctcct gatccttcgc tttggctgtg tctctgtata caggacgtac aggaagtgga 2461 aggaaaagat ccccaacccc agcaagagcc tcctgttcca ggatggaggt aaaggtctct 2521 ggcctcctgg cagcatggca gccttcgcca ctaagaaccc cgctctccag gggccacaga 2581 gcaggcttct tgctgagcaa cagggggagt catatgcaca tttggaagac aacaacgtgt 2641 cacctctcac tatagaggac cctaatataa ttcgagttcc accatccggg cctgatacaa 2701 ccccagctgc ctcatccgaa tccacagagc aacttcccaa tgttcaagta gagggaccaa 2761 ctcctaacag acctaggaag caattaccca gctttgactt caatgggccc tacctggggc 2821 ctccccaatc ccactctctg cctgatctcc cagaccagct gggttccccc caggtgggtg 2881 ggagcctgaa gccagcactg ccaggctcct tggagtacat gtgtctgccc cctggaggtc 2941 aagcgcaact ggttccattg tcccaggtga tggggcaggg ccaggctatg gatgtgcagt 3001 gtgggtccag cctggagacc tcagggagcc cttctgtgga gccaaaggag aaccctccag 3061 ttgagctgag catggaggaa caggaggcac gggacaaccc agtgactctg cccataagct 3121 ctgggggccc tgagggcagt atgatggcct ctgattatgt cactcctgga gatccggtgc 3181 tcactctgcc cacagggccc ctgtctacct ctctgggccc ctctctaggg ttgccctcag 3241 cccaaagccc cagtctctgt cttaagctgc ccagggtccc ctctggaagc ccagctctag 3301 ggccaccagg gtttgaggac tatgtggagc tgcctccaag tgtgagccag gctgccaagt 3361 cccctccagg ccatcctgct cctcctgtgg caagcagccc cacagtgatc ccaggagagc 3421 ccagggagga agtgggccca gcatccccac atcccgaagg cctccttgtt cttcagcagg 3481 ttggggacta ctgcttcctc cctggcctgg gacctggctc cctctcacca cacagtaagc 3541 caccctctcc aagtctgtgt tctgagactg aggacctagt ccaggacttg tctgtcaaaa 3601 agtttcccta tcagcccatg ccccaggcgc cagccattca gtttttcaag tccctaaagc 3661 atcaggacta cctgtccctg cccccttggg acaatagcca gtctgggaag gtgtgcacgc 3721 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc 3781 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc 3841 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 3901 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 3961 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 4021 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 4081 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 4141 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 4201 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 4261 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 4321 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg 4381 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca 4441 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt 4501 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5041 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5341 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac 5401 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga 5461 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg 5521 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt 5581 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct 5641 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc 5701 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa 5761 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct 5821 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata 5881 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg 5941 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat 6001 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg 6061 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg 6121 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta 6181 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc 6241 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga 6301 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca 6361 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc 6421 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc 6481 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt 6541 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca 6601 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc 6661 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc 6721 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg 6781 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag 6841 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc 6901 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 6961 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt 7021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7141 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 7201 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 7261 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 7321 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 7381 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 7441 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 7501 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 7561 gcgaatttt //