LOCUS       V032827                10833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032827
VERSION     V032827
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             744..1451
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1500..5495
                     /label="Cntnap2(NM_001004357)"
                     /note="Cntnap2(NM_001004357)"
                     /gene="Cntnap2"
     CDS             5496..5513
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             5520..5585
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5609..6197
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6263..6311
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6513..6941
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6955..7284
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7351..8145
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8321..8442
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8491..8507)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8515..8531
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8539..8569)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8584..8605
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8893..9481)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9652..10512)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10513..10617)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgcc accatggtga gcaagggcga ggaggataac atggccatca
      781 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1141 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1201 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1261 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1321 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1441 agctgtacaa gtggatcgga ggagggggaa gcggtggagg cggtagtgag ctcggatcca
     1501 tggtgatgtc tctgagagca ggctacaggg cagcgctctc gctctggatt ctcagcagct
     1561 tcatctgcag agcctggaca gctccctcca cgttccaaaa atgtgatgag cctctcatct
     1621 ctggtcttcc ccatgtgtcc ttcagcagct cttcctccct gtccagcagc tatgcacctg
     1681 gttatgccaa gattaataaa cgaggaggtg ctgggggatg gtctccatca gacagtgacc
     1741 actatcaatg gcttcaggtt gattttggta atcggaagca gatcagtgcc attgcaaccc
     1801 aaggaaggta cagcagttct gattgggtga cacaatatcg aatgctctac agtgacacag
     1861 ggagaaactg gaaaccctat catcaagatg gcaatatctg ggcttttcca ggaaacgtca
     1921 attctgacag cgtggtccga cacgacctgc agcatgcagt agttgcccgt tatgtacgcg
     1981 ttgtgccctt ggactggaat ggagaaggtc acatcgggct gcgtgcggaa gtctatggct
     2041 gcgcctactg ggctgacgtt atcaactttg atggccacgg tgtgttacca tatcgattca
     2101 gaaacaagaa gatgaaaaca ctcaaagatg ttattgcttt gaaatttaag acctctgaaa
     2161 gtgaaggagt acttttgcat ggtgaaggac agcaagggga ttacattact ttggaactga
     2221 aaaaagcaaa gctggtcctc agtttaaatc taggaagcaa ccagcttggc cccatctatg
     2281 gccacacatc tgtgacatct gggagcctgc tggatgatca ccactggcac tctgtactca
     2341 ttgagcgcca gggacgcagc attaacttga ccctggacag gagcatgcag cattttcgca
     2401 ccaatggaga gtttgactac ctggacttgg actatgagat aacatttgga ggcatacctt
     2461 tctctggtaa gccaagttcc agtaatagga agaacttcaa gggctgcatg gaaagcatca
     2521 actacaatgg tgtcaatatt accgatcttg cccgaagaaa gaaattagag ccatcaaata
     2581 tggggaatct gagtttctct tgtgtggagc cctatacagt accagtcttc ttcaatgcta
     2641 caagctacct ggaggtacct ggcagactta atcaggacct gttctcagtc agtttccagt
     2701 ttaggacttg gaaccccagc ggtctcttgc tcttcagtca ctttgcagat aacttgggca
     2761 atgtggagat tgaccttgta gagagcaaag tgggcgtcca cataaacaac acacagacca
     2821 agacaagcca aattgacatc tcctcaggct ccgggctgaa tgatgggcag tggcatgagg
     2881 ttcgcttcct ggccaaggaa aacttcgcag tcctcaccat tgatggagat gaagcatcag
     2941 cagttcggac caatagtcct cttcaagtga agactggcga gaaatacttc tttggaggat
     3001 ttctgaacca catgaacaat gccagctact ctgcccttca gccatcattc caaggatgca
     3061 tgcagctcat tcaggtagat gaccagcttg tgaatttata cgaagtagca cacaggaagc
     3121 caggaagctt cgcaaatgtc accattgaca tgtgtgccat catagacaga tgtgtgccca
     3181 atcactgtga gcatggtgga aagtgctcgc aaacatggga cagcttcaaa tgcacttgtg
     3241 atgagacagg atacagcggg gccacctgcc acaactccat ctacgagccc tcctgtgaag
     3301 catacaaaca ccttggccag acatcaaatt actactggat agatcctgat ggcagtggac
     3361 cactggggcc tctgaaggtt tattgcaaca tgacagagga caaggtgtgg accatagtgt
     3421 ctcatgactt acagatgcag acgacagtgg taggctacaa cccagaaaag tactctgtga
     3481 cacagcttat ctacagtgcc tccatggacc agattagtgc catcaccagt agcgccgagt
     3541 actgcgaaca gtacgtctcc tacttctgca ggatgtccag gttgttgaac accccagatg
     3601 gaagtcctta cacctggtgg gttggcaaag ccaatgagaa gcactactac tggggaggat
     3661 cagagcctgg aattcagaag tgtgcctgtg gcatagagcg caactgcaca gatcccaagt
     3721 actactgcaa ctgtgatgct gactacaagc agtggaggaa ggatgctggc ttcttatcct
     3781 acaaagatca cctgccagtg agccaggtgg tggttggaga tactgaccga caaggctcag
     3841 aagccaagct gagtgtgggt cccctgcgct gccaaggaga cagaaattat tggaatgctg
     3901 cctccttccc aaacccatca tcctacctgc acttctctac cttccaaggg gaaacaagtg
     3961 ctgacatttc tttctacttc aagacattaa tccccagggg agtatttttg gaaaatctgg
     4021 ggaacacaga cttcatcaag ctggagctca agtctgccac agaagtgtcc ttttcatttg
     4081 atgttggaaa cgggccagtg gagatcgtgg tgaggtcacc ttcacctctc aatgatgacc
     4141 agtggcatcg ggtcactgca gagaggaatg tcaagcaggc cagtctccag gtggatcgcc
     4201 tgccccagca aatccgaaaa gccccgactg aaggtcatac ccgcctggaa ctctacagtc
     4261 agctgtttgt tggtggtgct ggaggccagc agggcttcct aggctgcatc cgctccttga
     4321 ggatgaatgg agtaacactt gacttggaag aaagggcgaa ggtcacatct gggttcaagt
     4381 ctggatgctc aggccactgc accagctatg gggctaactg tgaaaatgga ggcaaatgca
     4441 tcgaaaaata ccatggctac tcctgcgact gctctaacac agcctatgat gggacattct
     4501 gcaacaaaga tgttggtgcc ttttttgaag aaggcatgtg gctgcgctat aacttccagg
     4561 ccccagcggt cactgccaga gacacaggca gcagggccga gaattctgca gaccagcagc
     4621 agcacttggc tccagatctg gcccaggagc agatccactt tagcttcagc actaccaagg
     4681 caccctgcat cctcctctat gtcagctccc tcaccactga cttcctagct gttctggtca
     4741 agcccacagg aaacttgcag atccggtata acctgggagg aaccagagag ccattcaata
     4801 ttgacgtaga ccacaggaac atggccaatg gacagccgca cagtgtcaac atcacccggc
     4861 atgagaggac cataattctc aagctcgacc attatcctgc tgtgggttac catctaccca
     4921 gctcctctga tacgctcttc aattctccca agtcgctctt ccttggaaaa gttatagaaa
     4981 cgggaaaaat cgaccaagaa attcacaaat acaacacccc aggcttcaca ggctgcctgt
     5041 ccagagtcca gttcaaccac atcgcccctc tcaaggcagc cttgagacag acaaacgcct
     5101 cagcccacgt gcacattcag ggtgagctgg tggaatctaa ctgcggggcc tccccactga
     5161 cgctttcccc catgtcttca gccactgacc cttggcacct agatcacttg gattcagcca
     5221 gtgctgattt cccatataac ccaggacaag gccaagctat aagaaatgga gtcaacagaa
     5281 actcagctat cattggaggg gtcatcgctg tggtgatttt caccatcctc tgcaccctgg
     5341 tcttcctcat ccggtacatg ttccgtcaca agggcaccta ccacaccaat gaggccaagg
     5401 gagctgagtc agccgagagt gcagatgctg ccatcatgaa caacgacccc aacttcacag
     5461 agaccattga cgagagcaaa aaggagtggc tcattcacca tcaccatcac catctcgagg
     5521 actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg
     5581 acaagtagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5641 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5701 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5761 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5821 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5881 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5941 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6001 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6061 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6121 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6181 ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa ggagacaata
     6241 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     6301 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6361 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     6421 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga
     6481 tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6661 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6841 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     6901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7201 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7261 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7321 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     7381 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7441 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7501 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7561 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7621 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7681 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7741 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7801 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7861 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7921 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7981 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     8041 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     8101 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     8161 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8221 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8281 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8461 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8581 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8641 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8701 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9061 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     9121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9301 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10621 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    10681 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    10741 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    10801 ttgaccgaca attgcatgaa gaatctgctt agg
//
