LOCUS       V031072                 5082 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031072
VERSION     V031072
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1229
                     /label="Ccdc179(NM_001195601)"
                     /note="Ccdc179(NM_001195601)"
                     /gene="Ccdc179"
     CDS             1248..1277
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1296..1319
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1339..1355)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1366..1988
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2137..2725)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3054..3101
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3333..4127)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4162..4519)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4521..4625)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4652..5080
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtgccttcga gttaaggatg aggagcctgc acaagtctac ccagaaggac
     1081 cacgaaggca ccatccttca gatgtttcta ccaggcagtc tgtggaaaaa cgtatcaatt
     1141 acatgcaaaa cctacagaaa gagaagagga aattggggaa aaggttcgca aggcctaacc
     1201 cgattccgga tacaggcatc ttgtggacaa cgcgtacgcg gccgctcgag cagaaactca
     1261 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     1321 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     1381 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     1441 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     1501 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     1561 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     1621 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     1681 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     1741 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     1801 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     1861 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     1921 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     1981 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2041 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2101 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2161 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2221 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2281 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2341 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2401 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2461 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2521 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2581 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2641 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2761 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2821 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2881 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     2941 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     3001 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     3061 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     3121 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     3181 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     3241 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     3301 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     3361 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     3421 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     3481 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     3541 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     3601 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     3661 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     3721 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     3781 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     3841 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     3901 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     3961 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     4021 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4081 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4141 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     4201 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4261 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4321 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     4381 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     4441 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     4501 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     4561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4621 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
