LOCUS V030853 5334 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030853 VERSION V030853 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1481 /label="Cav3(NM_007617)" /note="Cav3(NM_007617)" /gene="Cav3" CDS 1500..1529 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1548..1571 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1591..1607) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1618..2240 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2389..2977) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3306..3353 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3585..4379) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4414..4771) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4773..4877) /gene="bla" /label="AmpR promoter" rep_origin 4904..5332 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gatgaccgaa gagcacacgg atctggaagc tcggatcatc aaggacattc 1081 actgcaagga gatagacttg gtgaaccgcg accccaagaa catcaatgag gacattgtga 1141 aggtagattt tgaagacgtg attgcggagc ccgagggcac ctacagcttc gacggtgtat 1201 ggaaggtgag cttcaccacg ttcaccgtct ccaagtactg gtgctaccgc ctgttgtcta 1261 cactgctggg tgttccactg gccctgctct ggggattcct gttcgcctgt atctccttct 1321 gccacatctg ggccgtggtg ccctgcatta agagctacct gatcgagatc cagtgcatca 1381 gccacatcta ctcactgtgt atccgcacct tctgcaaccc gctcttcgct gcgttgggcc 1441 aggtctgcag caacattaag gtggtgctgc gaagggaagg cacgcgtacg cggccgctcg 1501 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg 1561 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatcccggg 1621 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg 1681 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc 1741 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc 1801 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc 1861 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg 1921 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt 1981 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg 2041 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt 2101 ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc 2161 caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact 2221 cagtagatgc ctgttgaatt gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa 2281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 2341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 2401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 2461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 2521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 2581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 2641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 2701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 2761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 2821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 2881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 2941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 3061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 3121 tatatatgag taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc 3181 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg 3241 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg 3301 tttatgaaca aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc 3361 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc 3421 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac 3481 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag 3541 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca 3601 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg 3661 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg 3721 tcctgatagc gatccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca 3781 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg 3841 tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct 3901 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 3961 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 4021 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc 4081 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc 4141 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc 4201 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 4261 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg 4321 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg 4381 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa 4441 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat 4501 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg 4561 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat 4621 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg 4681 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca 4741 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc 4801 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 4861 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc 4921 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 4981 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 5041 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 5101 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 5161 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 5221 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 5281 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa tttt //