LOCUS V031152 11766 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V031152 VERSION V031152 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..7913 /label="Cacna1g(NM_009783)" /note="Cacna1g(NM_009783)" /gene="Cacna1g" CDS 7932..7961 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 7980..8003 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(8023..8039) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 8050..8672 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(8821..9409) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 9738..9785 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(10017..10811) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(10846..11203) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(11205..11309) /gene="bla" /label="AmpR promoter" rep_origin 11336..11764 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat ggatgaggaa gaggatggag cgggcgccga ggagtcggga cagccccgga 1081 gcttcacgca gctcaacgac ctgtctgggg ccgggggccg gcaggggccg gggtcgacgg 1141 aaaaggaccc gggcagcgcg gactccgagg cggaggggct gccctacccg gcgctggccc 1201 cggtggtttt cttctacttg agccaggaca gccgcccgcg gagctggtgt ctccgcacgg 1261 tctgtaaccc gtggttcgag cgagtcagca tgctggttat tctcctcaac tgtgtgactc 1321 tgggtatgtt caggccgtgt gaggacattg cctgtgactc ccaacgctgc cggatcctgc 1381 aggccttcga cgacttcatc tttgccttct ttgctgtgga aatggtggtg aagatggtcg 1441 ctttgggtat ctttgggaag aaatgttacc tgggagacac ttggaaccgg cttgactttt 1501 ttatcgtcat tgctgggatg ctggagtact cgctggacct gcagaatgtc agcttctccg 1561 cagtcaggac agtccgtgtg ctgcgaccgc tcagggccat taaccgggtg cccagcatgc 1621 gcattctcgt cacattactg ctggatacct tgcctatgct gggcaatgtc ctgctgctct 1681 gtttcttcgt ctttttcatc tttggcatcg ttggtgttca gctgtgggca gggctacttc 1741 gaaaccgatg cttcctccct gagaatttca gcctcccgct gagtgtggac ttggagcctt 1801 actaccagac agagaatgag gacgagagcc ccttcatctg ctctcagcct cgggagaacg 1861 gcatgcgatc ctgcaggagc gtgcccacac tgcgcgggga aggcggtggt ggtccaccct 1921 gcggtctgga ctatgaggcc tataacagtt ccagcaatac cacctgtgtc aactggaacc 1981 aatactatac caactgctct gcaggcgagc acaacccctt caaaggcgcc atcaactttg 2041 acaacattgg ctatgcctgg atcgccatct tccaggtcat cacactggag ggctgggtcg 2101 acatcatgta ctttgtgatg gatgctcatt ccttctacaa cttcatctac ttcattcttc 2161 tcatcatcgt gggctccttc ttcatgatca acctgtgcct ggtggtgatc gccacacagt 2221 tctctgagac caagcaacgg gagagtcagc tgatgcggga gcagcgtgta cgattcctgt 2281 ccaatgctag caccctggca agcttctctg agccaggcag ctgctatgag gagcttctca 2341 agtacctggt gtacatcctc cgcaaagcag cccgcaggct ggcccaggtc tctagggctg 2401 taggcgtgcg ggctgggttg ctcagcagcc cagtggcccg tggtgggcag gagccccagc 2461 ccagtggcag ctgctctcgt tcacaccgtc gtctgtctgt ccaccacctg gtccaccacc 2521 atcaccacca ccatcaccac taccacctgg gtaatgggac gctcagagtt ccccgggcca 2581 gcccagagat ccaggacagg gatgccaatg ggtcccgctg gctcatgctg ccaccaccct 2641 ctacccccac tccctctggg ggccctccga ggggtgcgga gtctgtacac agcttctacc 2701 atgctgactg ccacttggag ccagtccgtt gccaggcgcc ccctcccagg tccccatcgg 2761 aggcatctgg caggactgtg ggtagtggga aggtataccc cactgtgcat accagccctc 2821 caccagagat gctgaaggat aaggcactag tggaggtggc ccccagccct gggcccccca 2881 ccctcaccag cttcaacatc ccacctgggc ccttcagctc catgcacaag ctcctggaga 2941 cacagagtac gggagcctgc catagctcct gcaaaatctc cagcccttgc tccaaggcag 3001 acagtggagc ctgtgggccg gacagttgtc cctactgtgc ccggacagga gcaggggagc 3061 cagagtccgc tgaccatgaa atgcctgact cagacagtga ggctgtgtat gagttcacac 3121 aggacgctca gcacagcgac ctccgggatc cccacagacg gcgacggccg agcctgggcc 3181 cagatgcaga gcctagttct gtgctggcct tctggaggct gatctgcgac acattccgga 3241 agatcgtaga tagcaaatac tttggccggg gaatcatgat cgctatcctg gtcaataccc 3301 tcagcatggg catcgagtac cacgagcagc ccgaggagct caccaacgcc ctggaaatca 3361 gcaacatcgt cttcaccagc ctcttcgcct tggagatgct gctgaagctg ctcgtctacg 3421 gtcccttcgg ctacattaag aatccctaca acatctttga tggcgtcatt gtggtcatca 3481 gcgtgtggga gattgtgggc cagcagggag gtggcctgtc ggtgctgcgg accttccgcc 3541 tgatgcgggt gctgaagcta gttcgcttcc tgccggcact gcagcggcag ctcgtggtgc 3601 tcatgaagac catggacaac gtggccacct tctgcatgct gctcatgctc ttcatcttca 3661 tcttcagcat cctgggcatg cacctttttg gttgcaagtt cgcatctgaa cgggatgggg 3721 acacgttgcc agaccggaag aattttgact ccctgctctg ggccattgtc actgtctttc 3781 agattctgac tcaggaagac tggaataaag tcctttacaa cggcatggcc tccacgtcat 3841 cttgggctgc tctttacttc atcgccctca tgacttttgg caactacgtg ctctttaacc 3901 tgcttgtcgc cattctcgtg gagggtttcc aggcagagga aatcggcaaa cgggaagata 3961 cgagtggaca gttaagctgt attcagctgc ctgtcaactc ccagggggga gatgccacca 4021 agtctgagtc agagcctgat ttcttttcgc ccagtgtgga tggtgatggg gacaggaaga 4081 agcgcttggc cctggtggcc ttgggagaac actcggaact acgaaagagc cttttgccac 4141 ctctcatcat ccacacagct gctacaccga tgtcactgcc caagagctcc agcacaggtg 4201 tgggggaagc actgggctct ggctctcgcc gcaccagtag cagtgggtcc gctgagcctg 4261 gaactgccca tcatgagatg aaatcaccgc caagtgcccg aagctccccg cacagtccct 4321 ggagcgcagc aagcagctgg accagcaggc gctccagccg gaacagcctg ggccgggccc 4381 ccagcctgaa gcgtaggagc ccaagcgggg agcggaggtc cctgctgtct ggagagggtc 4441 aggagagcca ggatgaggag gagagttcag aagaggaccg ggccagccca gcaggcagtg 4501 atcatcgcca caggggttcc ttggaacgtg aggccaagag ttcctttgac ctgcctgaca 4561 ccctgcaggt gcccgggctt catcgaacag ccagcggtcg gagctctgcc tctgaacacc 4621 aagactgtaa tggcaagtcg gcttcagggc gtttggcccg caccctgcgg gctgatgacc 4681 ccccactgga tggggatgat ggcgatgatg agggcaacct gagcaaaggg gaacgcttac 4741 gagcctgggt ccgagcccgg ctccctgcct gttgccgaga gcgagattcc tggtctgcct 4801 atatcttccc tccccagtca aggtttcgtc tcctgtgtca ccggatcatc acccacaaga 4861 tgtttgacca tgtggtcctc gtcatcatct tcctcaactg tatcaccatc gctatggagc 4921 gccccaaaat tgacccccac agcgctgaac gcatcttcct gaccctctcc aactacatct 4981 tcacggcagt cttcctggct gaaatgacag tgaaggtggt ggcactgggc tggtgctttg 5041 gggagcaggc ctacctgcgc agcagctgga acgtgctgga cggcttgctg gtgctcatct 5101 ctgtcatcga catcctggtg tccatggtct ctgacagcgg caccaagatt ctcggcatgc 5161 tgagggtgct gcggctgctg cggaccctac gtccactcag ggtcatcagc cgggcccagg 5221 ggctgaagct ggtggtagag actctgatgt catccctcaa acccattggc aacattgtgg 5281 tcatctgctg tgccttcttc atcatttttg gaattcttgg ggtgcagctc ttcaaaggga 5341 agttcttcgt gtgtcagggt gaggacacca ggaacatcac taacaagtcc gactgtgctg 5401 aggccagtta ccggtgggtc cggcacaagt acaactttga caacctgggc caggctctga 5461 tgtccctgtt tgtgctggcc tccaaggatg gctgggttga catcatgtat gatggactgg 5521 atgctgtggg agtggaccag cagcccatca tgaaccacaa cccttggatg ctgctctact 5581 tcatctcctt cctcctcatc gtggccttct tcgtcctgaa catgtttgtg ggcgtggtgg 5641 tggagaactt ccataagtgc aggcagcacc aggaggagga ggaggcgcgg cggcgggagg 5701 agaagcgact aaagaggctg gagaaaaaga gaaggagtaa ggagaagcag atggccgatc 5761 taatgttgga cgatgtaatt gcttccggca gctcagccag cgctgcgtca gaagcccagt 5821 gcaaacccta ctactctgac tactcgcgct tccggctcct cgtccaccac ctgtgtacca 5881 gccactacct ggacctcttc atcactggtg tcatcgggct gaatgtggtc acgatggcca 5941 tggaacatta ccagcagccc cagatcctgg acgaggctct gaagatctgc aactacatct 6001 ttaccgtcat ctttgtcttg gagtcagtat tcaaacttgt ggccttcggc ttccgccggt 6061 tcttccagga caggtggaac cagctggacc tggctattgt gcttctgtcc atcatgggca 6121 tcacgctgga agagattgag gtcaatgctt cactgcccat caaccccacc atcatccgta 6181 tcatgagggt gctccgcatt gctcgagttc tgaagctgtt gaagatggct gtgggcatgc 6241 gggcactgct ggacacggtg atgcaggccc tgccccaggt ggggaacctg ggacttctct 6301 tcatgctatt atttttcatc tttgcagctc tgggcgtgga gctctttgga gacctggagt 6361 gtgatgagac acacccttgt gagggcttgg gccggcatgc cacctttagg aactttggta 6421 tggcctttct gaccctcttc cgagtctcca ctggtgacaa ctggaatggt attatgaagg 6481 acaccctccg ggactgtgac caggagtcca cctgctacaa caccgtcatc tcacccatct 6541 acttcgtgtc cttcgtgctg acggcccagt ttgtgctggt caacgtggtc atagccgtgc 6601 tgatgaagca cctggaagag agcaacaaag aggccaagga ggaggcggag ttggaggcgg 6661 agctggagct agagatgaag acactcagcc cgcagcccca ctccccgctg ggcagcccct 6721 tcctctggcc tggggtggaa ggtgtcaata gccctgacag ccctaagcct ggggctccac 6781 acaccacggc ccacattgga gcagcctctt caggcttctc ccttgagcac cccacgatgg 6841 tacctcacac tgaggagggg ccagtccccc taggaccaga cctgctgact gtgaggaagt 6901 ctggtgtcag ccggacacac tctctgccca atgacagcta catgtgccgc aatgggagca 6961 ctgccgagag atccctagga cacaggggct gggggctccc caaagcccag tcaggctcca 7021 tcttgtctgt tcactcccaa ccagcagaca ccagctgcat cctacagctt cccaaagatg 7081 cacactatct gctccagcct catggggctc ccacctgggg cgccatccct aaactacccc 7141 cacctggccg ctcccctctg gctcagaggc ctctcaggcg ccaggcagca ataaggactg 7201 actccctgga cgtgcagggc ctgggtagcc gggaagacct gttgtcagag gtgagtgggc 7261 cctcctgccc tctgacccgc tcctcatcct tctggggcgg gtcgagcatc caggtgcagc 7321 agcgctccgg cagccagagc aaagtctcca agcacatccg cctgccagcc ccttgcccag 7381 gcctggaacc cagctgggcc aaggaccctc aagagaccag aagcagctta gagctggaca 7441 cggagctgag ctggatttca ggagacctcc tgcccagcag tcaggaagaa cccctgtccc 7501 cacgggactt gaaaaaatgc tacagtgtag aggcccagag ctgccggcgc aggcctgggt 7561 cctggctaga cgaacagagg agacactcca tcgctgtcag ctgcctggac agcggctccc 7621 agccccgcct atgtccaagc ccctcaagcc tcgggggcca acctcttggg ggccctggga 7681 gccggcctaa gaaaaaactc agcccaccca gtatctctat agaccccccg gagagccagg 7741 gccctcggcc cccatgcagt cctggcgtct gcctcaggag gagggcgccg gccagtgact 7801 cgaaggatcc ctcggcctcc agcccccttg acagcacggc tgcctcaccc tccccaaaga 7861 aagatgcgct gagtctctct ggtttgtctt ctgacccaac agacctggat cccacgcgta 7921 cgcggccgct cgagcagaaa ctcatctcag aagaggatct ggcagcaaat gatatcctgg 7981 attacaagga tgacgacgat aaggtttaaa cggccggccg cggtcatagc tgtttcctga 8041 acagatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt 8101 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac 8161 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt 8221 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca 8281 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct 8341 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg 8401 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc 8461 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct 8521 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta 8581 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc 8641 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcggc gagcggtatc 8701 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8761 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8821 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8881 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8941 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 9001 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 9061 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 9121 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 9181 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 9241 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 9301 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 9361 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 9421 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 9481 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 9541 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt 9601 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac 9661 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga 9721 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat 9781 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc 9841 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc 9901 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc 9961 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag 10021 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg 10081 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta 10141 gccaacgcta tgtcctgata gcgatccgcc acacccagcc ggccacagtc gatgaatcca 10201 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg 10261 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc 10321 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt 10381 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta 10441 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat 10501 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg 10561 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct 10621 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg 10681 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc 10741 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct 10801 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag 10861 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc 10921 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg 10981 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg 11041 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga 11101 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca 11161 ccctaactga cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa 11221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 11281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg 11341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 11401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 11461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 11521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 11581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 11641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 11701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 11761 aatttt //