LOCUS       V035383                13174 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035383
VERSION     V035383
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..7833
                     /label="CNOT1(NM_016284)"
                     /note="CNOT1(NM_016284)"
                     /gene="CNOT1"
     CDS             7840..7866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             7870..7896
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             7900..7926
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    7950..8538
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    8604..8652
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8854..9282
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9296..9625
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9692..10486
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10662..10783
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10832..10848)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10856..10872
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10880..10910)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10925..10946
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11234..11822)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11993..12853)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12854..12958)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgaa tcttgactcg
      721 ctctcgctgg ccttgtctca aatcagctac ctggtggaca atttaaccaa gaaaaattac
      781 cgagccagcc agcaggaaat acagcatatt gtgaatcggc acggtcctga ggcagacagg
      841 catttattac gctgcctatt ttcgcatgtg gatttcagtg gcgatggtaa aagcagtggc
      901 aaagatttcc atcagactca gtttctgatt caggagtgtg cgttgctgat tacaaagcca
      961 aattttatct cgacgctgtc ctatgccatt gataatccat tgcactatca gaagagttta
     1021 aagcctgcac cccacttatt tgcccagctg agtaaagtgc tcaaattaag caaagtacaa
     1081 gaggtaattt ttggccttgc cctgttgaat tcttccagct cagatcttag aggtttcgct
     1141 gcccagttta tcaaacagaa gcttccagat cttctgcgtt cttacattga cgcagacgtc
     1201 agtggaaatc aagaaggtgg cttccaagat atagcaatag aggtcctaca cctcctcctc
     1261 tcccatctcc tctttgggca gaagggagcc tttggagttg gacaagaaca gatagacgct
     1321 tttcttaaga cgctgcgcag agattttccc caagaacgct gtcccgtggt gctcgcacca
     1381 cttttatacc ctgaaaaacg ggacattcta atggacagga tcctgcctga ttccggaggg
     1441 gtagctaaaa ccatgatgga gagctctttg gctgatttca tgcaagaagt aggctatggc
     1501 ttttgtgcaa gtattgaaga atgtcgcaat ataatcgtgc agtttggtgt tcgggaggtc
     1561 acagctgccc aggttgcaag ggttttggga atgatggctc gaactcattc aggattaaca
     1621 gatggcattc cattacagag tatttctgct ccgggcagtg ggatctggag tgatgggaaa
     1681 gataaaagtg atggagcaca ggcacacaca tggaatgtag aagtcttgat tgacgttctt
     1741 aaagaactga atccaagttt gaatttcaag gaagtaactt atgaactgga ccatcctgga
     1801 tttcaaattc gtgacagtaa aggacttcat aatgtggttt atggcattca gaggggtttg
     1861 ggtatggaag tgttcccagt agacctcata tatagacctt ggaaacatgc tgaaggccag
     1921 ctctccttca ttcaacattc ccttataaat ccagagatct tctgttttgc tgactatccc
     1981 tgtcatactg ttgccactga tattctgaaa gcaccaccag aggatgacaa tcgagaaatt
     2041 gccacatgga agagcttgga tttgattgaa tctctgctga ggcttgcaga ggttgggcag
     2101 tatgagcaag tcaaacagct cttcagcttc cctatcaaac actgtccaga catgctggta
     2161 ttggccttac tacaaattaa cacctcttgg cataccttgc gccatgaact tatctccact
     2221 ctgatgccaa ttttccttgg aaaccatcct aactcagcta ttattttgca ctatgcatgg
     2281 catgggcagg gacagtctcc ctcaattcgc caacttatca tgcatgcaat ggcagaatgg
     2341 tacatgagag gggagcagta tgatcaggcc aaattgtctc gaatacttga tgtggcccag
     2401 gacttgaagg ccttgtcaat gctgctaaat ggtactccat ttgcctttgt tattgacctt
     2461 gctgcacttg cttcacgtcg tgaatacctc aaacttgata agtggctcac agataaaatt
     2521 cgagagcatg gggagccttt tatccaggcg tgtatgactt ttttaaagag acggtgtcct
     2581 tctattttgg gcggacttgc cccagaaaaa gaccagccca aaagtgctca acttcctcca
     2641 gaaactttgg cgacaatgtt ggcctgtctg caagcttgtg cagggagtgt ttctcaggag
     2701 ctatcagaaa ctatcctcac catggtagcc aattgcagta atgttatgaa taaggccaga
     2761 caaccaccac ctggagttat gccaaaagga cgtcctccta gtgctagcag cttagatgcc
     2821 atttctcctg ttcagattga ccctcttgct ggaatgacat ctcttagtat aggtggttca
     2881 gctgcccctc acacccagag tatgcagggt tttcctccaa atttgggttc tgcattcagt
     2941 acccctcagt caccagcaaa agcatttcca cccctttcaa cccccaatca gaccactgca
     3001 ttcagtggta ttggaggact ttcatcacag cttccagtag gtggtcttgg cacaggcagc
     3061 ctgactggta taggaactgg tgctcttgga ctccctgcag tgaataacga cccttttgta
     3121 cagaggaaac tgggcacctc tggactgaat cagcctacat tccagcagag taagatgaaa
     3181 ccttcggact tgtctcaggt gtggccagag gcaaaccagc actttagtaa agagatagat
     3241 gatgaagcaa acagctattt ccagcgaata tataatcatc caccacatcc aaccatgtct
     3301 gttgatgagg tattagaaat gctgcagaga tttaaagact ctactataaa gagggaacga
     3361 gaagtattta actgtatgct aaggaacttg tttgaagaat atcgtttttt tccccagtat
     3421 cctgataaag agttacatat aacagcctgc ctatttggtg gtataattga gaaaggactg
     3481 gtcacttaca tggcactagg tctggctcta cgatatgttc ttgaagcctt acgcaagcct
     3541 tttggatcca aaatgtatta tttcgggatt gctgcactag atagatttaa aaacagattg
     3601 aaggactatc cccagtattg tcagcatttg gcttctatca gtcactttat gcaatttcca
     3661 catcatttac aggagtatat tgagtatgga cagcagtcta gagatcctcc tgtgaaaatg
     3721 caaggctcta tcacaacccc tggaagtatt gcactggctc aggcccaggc tcaggcccag
     3781 gttccagcaa aagctcctct tgctggtcaa gttagcacta tggtaaccac ctcaacaact
     3841 accactgttg ctaaaacggt tacggtcacc aggccaactg gagtcagctt taagaaagat
     3901 gtgccacctt ctattaatac tacaaatata gatacgttgc ttgtggccac agatcaaact
     3961 gagagaattg tggagccccc agaaaatatc caggagaaaa ttgcttttat tttcaataat
     4021 ctctcacagt caaatatgac acaaaaggtt gaagagctaa aggaaacggt gaaagaagaa
     4081 tttatgcctt gggtttcaca gtatctggtt atgaagagag tcagtattga gccaaacttt
     4141 catagcctgt attcaaactt ccttgacacg ctgaagaatc ctgaatttaa caagatggtt
     4201 ctgaatgaga cctacagaaa cattaaagtg ctcctgacct ctgataaagc tgcagccaat
     4261 ttctcagatc gttctttgct gaagaacttg ggacattggc taggaatgat cacattagct
     4321 aaaaacaaac ccatcttaca cactgacttg gatgtgaaat cattgctgct agaggcttat
     4381 gttaaaggac aacaagaatt gctctatgta gtgccctttg ttgccaaagt cttagaatct
     4441 agcattcgta gtgtggtttt taggccacca aacccttgga caatggcaat tatgaatgta
     4501 ttagctgagc tacatcagga gcatgactta aagttaaact tgaagtttga aatcgaggtt
     4561 ctctgcaaga accttgcatt agacatcaat gagctaaaac ctggaaacct cctaaaggat
     4621 aaagatcgcc tgaagaattt agatgagcaa ctctctgctc caaagaaaga tgtcaagcag
     4681 ccagaagaac tccctcccat cacaaccaca acaacttcta ctacaccagc taccaacacc
     4741 acttgtacag ccacggttcc accacagcca cagtacagct accacgacat caatgtctat
     4801 tcccttgcgg gcttggcacc acacattact ctgaatccaa caattccctt gtttcaggcc
     4861 catccacagt tgaagcagtg tgtgcgtcag gcaattgaac gggctgtcca ggagctggtc
     4921 catcctgtgg tggatcgatc aattaagatt gccatgacta cttgtgagca aatagtcagg
     4981 aaggattttg ccctggattc ggaggaatct cgaatgcgaa tagcagctca tcacatgatg
     5041 cgtaacttga cagctggaat ggctatgatt acatgcaggg aacctttgct catgagcata
     5101 tctaccaact taaaaaacag ttttgcctca gcccttcgta ctgcttcccc acaacaaaga
     5161 gaaatgatgg atcaggcagc tgctcaatta gctcaggaca attgtgagtt ggcttgctgt
     5221 tttattcaga agactgcagt agaaaaagca ggccctgaga tggacaagag attagcaact
     5281 gaatttgagc tgagaaaaca tgctaggcaa gaaggacgca gatactgtga tcctgttgtt
     5341 ttaacatatc aagctgaacg gatgccagag caaatcaggc tgaaagttgg tggtgtggac
     5401 ccaaagcagt tggctgttta cgaagagttt gcacgcaatg ttcctggctt cttacctaca
     5461 aatgacttaa gtcagcccac gggattttta gcccagccca tgaagcaagc ttgggcaaca
     5521 gatgatgtag ctcagattta tgataagtgt attacagaac tggagcaaca tctacatgcc
     5581 atcccaccaa ctttggccat gaaccctcaa gctcaggctc ttcgaagtct cttggaggtt
     5641 gtagttttat ctcgaaactc tcgggatgcc atagctgctc ttggattgct ccaaaaggct
     5701 gtagagggct tactagatgc cacaagtggt gctgatgctg accttctgct gcgctacagg
     5761 gaatgccacc tcttggtcct aaaagctctg caggatggcc gggcatatgg gtctccatgg
     5821 tgcaacaaac agatcacaag gtgcctaatt gaatgtcgag atgaatataa atataatgtg
     5881 gaggctgtgg agctgctaat tcgcaatcat ttggttaata tgcagcagta tgatcttcac
     5941 ctagcgcagt caatggagaa tggcttaaac tacatggctg tggcatttgc tatgcagtta
     6001 gtaaaaatcc tgctggtgga tgaaaggagt gttgctcatg ttactgaggc agatctgttc
     6061 cacaccattg aaaccctcat gaggattaat gctcattcca gaggcaatgc tccagaagga
     6121 ttgccccagc tgatggaagt agtgcgatcc aactatgaag caatgattga tcgtgctcat
     6181 ggaggcccaa actttatgat gcattctggg atctctcaag cctcagagta tgatgaccct
     6241 ccaggcctga gggagaaggc agagtatctt ctgagggaat gggtgaatct ctaccattca
     6301 gcagcagctg gccgcgacag taccaaagct ttctctgcat ttgttggaca gatgcaccag
     6361 caaggaatac tgaagaccga tgatctcata acaaggttct ttcgtctgtg tactgaaatg
     6421 tgtgttgaaa tcagttaccg tgctcaggct gagcagcagc acaatcctgc tgccaatccc
     6481 accatgatcc gagccaagtg ctatcacaac ctggatgcct ttgttcgact cattgcactg
     6541 ctcgtgaaac actcagggga ggccaccaac actgtcacaa agattaatct gctgaacaag
     6601 gtccttggta tagtagtggg agttctcctt caggatcatg atgttcgtca gagtgaattt
     6661 cagcaacttc cctaccatcg aatttttatc atgcttctct tggaactcaa tgcacctgag
     6721 catgtgttgg aaaccattaa tttccagaca cttacagctt tctgcaatac attccacatc
     6781 ttgaggccta ccaaagctcc tggctttgta tatgcctggc ttgaactgat ttcccatcgg
     6841 atatttattg caagaatgct ggcacatacg ccacagcaga aggggtggcc tatgtatgca
     6901 cagctactga ttgatttatt caaatattta gcgcctttcc ttagaaatgt ggaactcacc
     6961 aaacctatgc aaatcctcta caagggcact ttaagagtgc tgctggttct tttgcatgat
     7021 ttcccagagt tcctttgtga ttaccattat gggttctgtg atgtgatccc acctaattgt
     7081 atccagttaa gaaatttgat cctgagtgcc tttccaagaa acatgaggct ccccgaccca
     7141 ttcactccta atctaaaggt ggacatgttg agtgaaatta acattgctcc ccggattctc
     7201 accaatttca ctggagtaat gccacctcag ttcaaaaagg atttggattc ctatcttaaa
     7261 actcgatcac cagtcacttt cctgtctgat ctgcgcagca acctacaggt atccaatgaa
     7321 cctgggaatc gctacaacct ccagctcatc aatgcactgg tgctctatgt cgggactcag
     7381 gccattgcgc acatccacaa caagggcagc acaccttcaa tgagcaccat cactcactca
     7441 gcacacatgg atatcttcca gaatttggct gtggacttgg acactgaggg tcgctatctc
     7501 tttttgaatg caattgcaaa tcagctccgg tacccaaata gccacactca ctacttcagt
     7561 tgcaccatgc tgtacctttt tgcagaggcc aatacggaag ccatccaaga acagatcaca
     7621 agagttctct tggaacggtt gattgtaaat aggccacatc cttggggtct tcttattacc
     7681 ttcattgagc tgattaaaaa cccagcgttt aagttctgga accatgaatt tgtacactgt
     7741 gccccagaaa tcgaaaagtt attccagtcg gtcgcacagt gctgcatggg acagaagcag
     7801 gcccagcaag taatggaagg gacaggtgcc agtctcgagt acccatacga cgtacctgat
     7861 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     7921 tacgcatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     7981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8341 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8521 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     8581 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     8641 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     8701 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     8761 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     8821 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     8881 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8941 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9001 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9061 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9121 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9181 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     9241 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9301 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9361 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9421 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9481 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9541 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9601 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9661 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     9721 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9781 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9841 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     9901 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     9961 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10021 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10081 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10141 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10201 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10261 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10321 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10381 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10441 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10501 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
    10561 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10621 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10681 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10741 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10801 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    11041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11401 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
    11461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11641 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    11701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    12001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    12061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12961 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    13021 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    13081 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    13141 cttgaccgac aattgcatga agaatctgct tagg
//
