LOCUS V043255 9439 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043255 VERSION V043255 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..4098 /label="CIITA(NM_001379332)" /note="CIITA(NM_001379332)" /gene="CIITA" CDS 4105..4131 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4135..4161 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4165..4191 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 4215..4803 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4869..4917 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5119..5547 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5561..5890 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5957..6751 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6927..7048 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7097..7113) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7121..7137 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7145..7175) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7190..7211 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7499..8087) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8258..9118) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9119..9223) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcg ttgcctggct 721 ccacgccctg ctgggtccta cctgtcagag ccccaaggca gctcacagtg tgccaccatg 781 gagttggggc ccctagaagg tggctacctg gagcttctta acagcgatgc tgaccccctg 841 tgcctctacc acttctatga ccagatggac ctggctggag aagaagagat tgagctctac 901 tcagaacccg acacagacac catcaactgc gaccagttca gcaggctgtt gtgtgacatg 961 gaaggtgatg aagagaccag ggaggcttat gccaatatcg cggaactgga ccagtatgtc 1021 ttccaggact cccagctgga gggcctgagc aaggacattt tcatagagca cataggacca 1081 gatgaagtga tcggtgagag tatggagatg ccagcagaag ttgggcagaa aagtcagaaa 1141 agacccttcc cagaggagct tccggcagac ctgaagcact ggaagccagc tgagcccccc 1201 actgtggtga ctggcagtct cctagtggga ccagtgagcg actgctccac cctgccctgc 1261 ctgccactgc ctgcgctgtt caaccaggag ccagcctccg gccagatgcg cctggagaaa 1321 accgaccaga ttcccatgcc tttctccagt tcctcgttga gctgcctgaa tctccctgag 1381 ggacccatcc agtttgtccc caccatctcc actctgcccc atgggctctg gcaaatctct 1441 gaggctggaa caggggtctc cagtatattc atctaccatg gtgaggtgcc ccaggccagc 1501 caagtacccc ctcccagtgg attcactgtc cacggcctcc caacatctcc agaccggcca 1561 ggctccacca gccccttcgc tccatcagcc actgacctgc ccagcatgcc tgaacctgcc 1621 ctgacctccc gagcaaacat gacagagcac aagacgtccc ccacccaatg cccggcagct 1681 ggagaggtct ccaacaagct tccaaaatgg cctgagccgg tggagcagtt ctaccgctca 1741 ctgcaggaca cgtatggtgc cgagcccgca ggcccggatg gcatcctagt ggaggtggat 1801 ctggtgcagg ccaggctgga gaggagcagc agcaagagcc tggagcggga actggccacc 1861 ccggactggg cagaacggca gctggcccaa ggaggcctgg ctgaggtgct gttggctgcc 1921 aaggagcacc ggcggccgcg tgagacacga gtgattgctg tgctgggcaa agctggtcag 1981 ggcaagagct attgggctgg ggcagtgagc cgggcctggg cttgtggccg gcttccccag 2041 tacgactttg tcttctctgt cccctgccat tgcttgaacc gtccggggga tgcctatggc 2101 ctgcaggatc tgctcttctc cctgggccca cagccactcg tggcggccga tgaggttttc 2161 agccacatct tgaagagacc tgaccgcgtt ctgctcatcc tagacggctt cgaggagctg 2221 gaagcgcaag atggcttcct gcacagcacg tgcggaccgg caccggcgga gccctgctcc 2281 ctccgggggc tgctggccgg ccttttccag aagaagctgc tccgaggttg caccctcctc 2341 ctcacagccc ggccccgggg ccgcctggtc cagagcctga gcaaggccga cgccctattt 2401 gagctgtccg gcttctccat ggagcaggcc caggcatacg tgatgcgcta ctttgagagc 2461 tcagggatga cagagcacca agacagagcc ctgacgctcc tccgggaccg gccacttctt 2521 ctcagtcaca gccacagccc tactttgtgc cgggcagtgt gccagctctc agaggccctg 2581 ctggagcttg gggaggacgc caagctgccc tccacgctca cgggactcta tgtcggcctg 2641 ctgggccgtg cagccctcga cagccccccc ggggccctgg cagagctggc caagctggcc 2701 tgggagctgg gccgcagaca tcaaagtacc ctacaggagg accagttccc atccgcagac 2761 gtgaggacct gggcgatggc caaaggctta gtccaacacc caccgcgggc cgcagagtcc 2821 gagctggcct tccccagctt cctcctgcaa tgcttcctgg gggccctgtg gctggctctg 2881 agtggcgaaa tcaaggacaa ggagctcccg cagtacctag cattgacccc aaggaagaag 2941 aggccctatg acaactggct ggagggcgtg ccacgctttc tggctgggct gatcttccag 3001 cctcccgccc gctgcctggg agccctactc gggccatcgg cggctgcctc ggtggacagg 3061 aagcagaagg tgcttgcgag gtacctgaag cggctgcagc cggggacact gcgggcgcgg 3121 cagctgctgg agctgctgca ctgcgcccac gaggccgagg aggctggaat ttggcagcac 3181 gtggtacagg agctccccgg ccgcctctct tttctgggca cccgcctcac gcctcctgat 3241 gcacatgtac tgggcaaggc cttggaggcg gcgggccaag acttctccct ggacctccgc 3301 agcactggca tttgcccctc tggattgggg agcctcgtgg gactcagctg tgtcacccgt 3361 ttcagggctg ccttgagcga cacggtggcg ctgtgggagt ccctgcagca gcatggggag 3421 accaagctac ttcaggcagc agaggagaag ttcaccatcg agcctttcaa agccaagtcc 3481 ctgaaggatg tggaagacct gggaaagctt gtgcagactc agaggacgag aagttcctcg 3541 gaagacacag ctggggagct ccctgctgtt cgggacctaa agaaactgga gtttgcgctg 3601 ggccctgtct caggccccca ggctttcccc aaactggtgc ggatcctcac ggccttttcc 3661 tccctgcagc atctggacct ggatgcgctg agtgagaaca agatcgggga cgagggtgtc 3721 tcgcagctct cagccacctt cccccagctg aagtccttgg aaaccctcaa tctgtcccag 3781 aacaacatca ctgacctggg tgcctacaaa ctcgccgagg ccctgccttc gctcgctgca 3841 tccctgctca ggctaagctt gtacaataac tgcatctgcg acgtgggagc cgagagcttg 3901 gctcgtgtgc ttccggacat ggtgtccctc cgggtgatgg acgtccagta caacaagttc 3961 acggctgccg gggcccagca gctcgctgcc agccttcgga ggtgtcctca tgtggagacg 4021 ctggcgatgt ggacgcccac catcccattc agtgtccagg aacacctgca acaacaggat 4081 tcacggatca gcctgagact cgagtaccca tacgacgtac ctgattatgc aacttatcct 4141 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagtgaggg 4201 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 4261 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 4321 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 4381 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 4441 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4501 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 4561 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 4621 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 4681 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 4741 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 4801 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 4861 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata 4921 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 4981 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 5041 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg 5101 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 5161 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 5221 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc 5281 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 5341 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 5401 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 5461 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc 5521 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 5581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 5641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 5701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 5761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 5821 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 5881 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 5941 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 6001 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 6061 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 6121 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 6181 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 6241 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 6301 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 6361 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 6421 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 6481 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 6541 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 6601 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 6661 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 6721 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 6781 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 6841 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 6901 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 6961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 7021 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 7081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7681 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9241 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac 9301 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 9361 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 9421 catgaagaat ctgcttagg //