LOCUS       V043255                 9439 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043255
VERSION     V043255
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4098
                     /label="CIITA(NM_001379332)"
                     /note="CIITA(NM_001379332)"
                     /gene="CIITA"
     CDS             4105..4131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4135..4161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4165..4191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4215..4803
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4869..4917
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5119..5547
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5561..5890
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5957..6751
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6927..7048
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7097..7113)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7121..7137
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7145..7175)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7190..7211
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7499..8087)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8258..9118)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9119..9223)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgcg ttgcctggct
      721 ccacgccctg ctgggtccta cctgtcagag ccccaaggca gctcacagtg tgccaccatg
      781 gagttggggc ccctagaagg tggctacctg gagcttctta acagcgatgc tgaccccctg
      841 tgcctctacc acttctatga ccagatggac ctggctggag aagaagagat tgagctctac
      901 tcagaacccg acacagacac catcaactgc gaccagttca gcaggctgtt gtgtgacatg
      961 gaaggtgatg aagagaccag ggaggcttat gccaatatcg cggaactgga ccagtatgtc
     1021 ttccaggact cccagctgga gggcctgagc aaggacattt tcatagagca cataggacca
     1081 gatgaagtga tcggtgagag tatggagatg ccagcagaag ttgggcagaa aagtcagaaa
     1141 agacccttcc cagaggagct tccggcagac ctgaagcact ggaagccagc tgagcccccc
     1201 actgtggtga ctggcagtct cctagtggga ccagtgagcg actgctccac cctgccctgc
     1261 ctgccactgc ctgcgctgtt caaccaggag ccagcctccg gccagatgcg cctggagaaa
     1321 accgaccaga ttcccatgcc tttctccagt tcctcgttga gctgcctgaa tctccctgag
     1381 ggacccatcc agtttgtccc caccatctcc actctgcccc atgggctctg gcaaatctct
     1441 gaggctggaa caggggtctc cagtatattc atctaccatg gtgaggtgcc ccaggccagc
     1501 caagtacccc ctcccagtgg attcactgtc cacggcctcc caacatctcc agaccggcca
     1561 ggctccacca gccccttcgc tccatcagcc actgacctgc ccagcatgcc tgaacctgcc
     1621 ctgacctccc gagcaaacat gacagagcac aagacgtccc ccacccaatg cccggcagct
     1681 ggagaggtct ccaacaagct tccaaaatgg cctgagccgg tggagcagtt ctaccgctca
     1741 ctgcaggaca cgtatggtgc cgagcccgca ggcccggatg gcatcctagt ggaggtggat
     1801 ctggtgcagg ccaggctgga gaggagcagc agcaagagcc tggagcggga actggccacc
     1861 ccggactggg cagaacggca gctggcccaa ggaggcctgg ctgaggtgct gttggctgcc
     1921 aaggagcacc ggcggccgcg tgagacacga gtgattgctg tgctgggcaa agctggtcag
     1981 ggcaagagct attgggctgg ggcagtgagc cgggcctggg cttgtggccg gcttccccag
     2041 tacgactttg tcttctctgt cccctgccat tgcttgaacc gtccggggga tgcctatggc
     2101 ctgcaggatc tgctcttctc cctgggccca cagccactcg tggcggccga tgaggttttc
     2161 agccacatct tgaagagacc tgaccgcgtt ctgctcatcc tagacggctt cgaggagctg
     2221 gaagcgcaag atggcttcct gcacagcacg tgcggaccgg caccggcgga gccctgctcc
     2281 ctccgggggc tgctggccgg ccttttccag aagaagctgc tccgaggttg caccctcctc
     2341 ctcacagccc ggccccgggg ccgcctggtc cagagcctga gcaaggccga cgccctattt
     2401 gagctgtccg gcttctccat ggagcaggcc caggcatacg tgatgcgcta ctttgagagc
     2461 tcagggatga cagagcacca agacagagcc ctgacgctcc tccgggaccg gccacttctt
     2521 ctcagtcaca gccacagccc tactttgtgc cgggcagtgt gccagctctc agaggccctg
     2581 ctggagcttg gggaggacgc caagctgccc tccacgctca cgggactcta tgtcggcctg
     2641 ctgggccgtg cagccctcga cagccccccc ggggccctgg cagagctggc caagctggcc
     2701 tgggagctgg gccgcagaca tcaaagtacc ctacaggagg accagttccc atccgcagac
     2761 gtgaggacct gggcgatggc caaaggctta gtccaacacc caccgcgggc cgcagagtcc
     2821 gagctggcct tccccagctt cctcctgcaa tgcttcctgg gggccctgtg gctggctctg
     2881 agtggcgaaa tcaaggacaa ggagctcccg cagtacctag cattgacccc aaggaagaag
     2941 aggccctatg acaactggct ggagggcgtg ccacgctttc tggctgggct gatcttccag
     3001 cctcccgccc gctgcctggg agccctactc gggccatcgg cggctgcctc ggtggacagg
     3061 aagcagaagg tgcttgcgag gtacctgaag cggctgcagc cggggacact gcgggcgcgg
     3121 cagctgctgg agctgctgca ctgcgcccac gaggccgagg aggctggaat ttggcagcac
     3181 gtggtacagg agctccccgg ccgcctctct tttctgggca cccgcctcac gcctcctgat
     3241 gcacatgtac tgggcaaggc cttggaggcg gcgggccaag acttctccct ggacctccgc
     3301 agcactggca tttgcccctc tggattgggg agcctcgtgg gactcagctg tgtcacccgt
     3361 ttcagggctg ccttgagcga cacggtggcg ctgtgggagt ccctgcagca gcatggggag
     3421 accaagctac ttcaggcagc agaggagaag ttcaccatcg agcctttcaa agccaagtcc
     3481 ctgaaggatg tggaagacct gggaaagctt gtgcagactc agaggacgag aagttcctcg
     3541 gaagacacag ctggggagct ccctgctgtt cgggacctaa agaaactgga gtttgcgctg
     3601 ggccctgtct caggccccca ggctttcccc aaactggtgc ggatcctcac ggccttttcc
     3661 tccctgcagc atctggacct ggatgcgctg agtgagaaca agatcgggga cgagggtgtc
     3721 tcgcagctct cagccacctt cccccagctg aagtccttgg aaaccctcaa tctgtcccag
     3781 aacaacatca ctgacctggg tgcctacaaa ctcgccgagg ccctgccttc gctcgctgca
     3841 tccctgctca ggctaagctt gtacaataac tgcatctgcg acgtgggagc cgagagcttg
     3901 gctcgtgtgc ttccggacat ggtgtccctc cgggtgatgg acgtccagta caacaagttc
     3961 acggctgccg gggcccagca gctcgctgcc agccttcgga ggtgtcctca tgtggagacg
     4021 ctggcgatgt ggacgcccac catcccattc agtgtccagg aacacctgca acaacaggat
     4081 tcacggatca gcctgagact cgagtaccca tacgacgtac ctgattatgc aacttatcct
     4141 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagtgaggg
     4201 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4261 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4321 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4381 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4441 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4501 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4561 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4621 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4681 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4741 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4801 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4861 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     4921 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     4981 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     5041 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg
     5101 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5161 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5221 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     5281 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5341 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5401 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5461 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     5521 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5821 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5881 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5941 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6001 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6061 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6121 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6181 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6241 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6301 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6361 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6421 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6481 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6541 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6601 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6661 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6721 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg
     6781 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     6841 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6901 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7021 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7681 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9241 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac
     9301 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     9361 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     9421 catgaagaat ctgcttagg
//
