LOCUS       V035181                 6449 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035181
VERSION     V035181
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1007..2608
                     /label="CD276(NM_001024736)"
                     /note="CD276(NM_001024736)"
                     /gene="CD276"
     CDS             2615..2644
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2663..2686
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2706..2722)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2733..3355
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3504..4092)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4421..4468
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4700..5494)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5529..5886)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5888..5992)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6019..6447
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tctcgaggcg atcgccatgc tgcgtcggcg
     1021 gggcagccct ggcatgggtg tgcatgtggg tgcagccctg ggagcactgt ggttctgcct
     1081 cacaggagcc ctggaggtcc aggtccctga agacccagtg gtggcactgg tgggcaccga
     1141 tgccaccctg tgctgctcct tctcccctga gcctggcttc agcctggcac agctcaacct
     1201 catctggcag ctgacagata ccaaacagct ggtgcacagc tttgctgagg gccaggacca
     1261 gggcagcgcc tatgccaacc gcacggccct cttcccggac ctgctggcac agggcaacgc
     1321 atccctgagg ctgcagcgcg tgcgtgtggc ggacgagggc agcttcacct gcttcgtgag
     1381 catccgggat ttcggcagcg ctgccgtcag cctgcaggtg gccgctccct actcgaagcc
     1441 cagcatgacc ctggagccca acaaggacct gcggccaggg gacacggtga ccatcacgtg
     1501 ctccagctac cagggctacc ctgaggctga ggtgttctgg caggatgggc agggtgtgcc
     1561 cctgactggc aacgtgacca cgtcgcagat ggccaacgag cagggcttgt ttgatgtgca
     1621 cagcatcctg cgggtggtgc tgggtgcaaa tggcacctac agctgcctgg tgcgcaaccc
     1681 cgtgctgcag caggatgcgc acagctctgt caccatcaca ccccagagaa gccccacagg
     1741 agccgtggag gtccaggtcc ctgaggaccc ggtggtggcc ctagtgggca ccgatgccac
     1801 cctgcgctgc tccttctccc ccgagcctgg cttcagcctg gcacagctca acctcatctg
     1861 gcagctgaca gacaccaaac agctggtgca cagtttcacc gaaggccggg accagggcag
     1921 cgcctatgcc aaccgcacgg ccctcttccc ggacctgctg gcacaaggca atgcatccct
     1981 gaggctgcag cgcgtgcgtg tggcggacga gggcagcttc acctgcttcg tgagcatccg
     2041 ggatttcggc agcgctgccg tcagcctgca ggtggccgct ccctactcga agcccagcat
     2101 gaccctggag cccaacaagg acctgcggcc aggggacacg gtgaccatca cgtgctccag
     2161 ctaccggggc taccctgagg ctgaggtgtt ctggcaggat gggcagggtg tgcccctgac
     2221 tggcaacgtg accacgtcgc agatggccaa cgagcagggc ttgtttgatg tgcacagcgt
     2281 cctgcgggtg gtgctgggtg cgaatggcac ctacagctgc ctggtgcgca accccgtgct
     2341 gcagcaggat gcgcacggct ctgtcaccat cacagggcag cctatgacat tccccccaga
     2401 ggccctgtgg gtgaccgtgg ggctgtctgt ctgtctcatt gcactgctgg tggccctggc
     2461 tttcgtgtgc tggagaaaga tcaaacagag ctgtgaggag gagaatgcag gagctgagga
     2521 ccaggatggg gagggagaag gctccaagac agccctgcag cctctgaaac actctgacag
     2581 caaagaagat gatggacaag aaatagccgg atccgagcag aaactcatct cagaagagga
     2641 tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt aaacggccgg
     2701 ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac ccctccccag
     2761 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     2821 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     2881 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     2941 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     3001 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     3061 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     3121 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     3181 cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac cagcaggagg
     3241 acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat ttgagttgct
     3301 tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt gaattgggta
     3361 cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3901 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg
     4261 gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg
     4321 tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta
     4381 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg
     4441 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     4501 ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg
     4561 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
     4621 aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt
     4681 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     4741 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     4801 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc gccacaccca
     4861 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     4921 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     4981 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     5041 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     5101 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     5161 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     5221 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     5281 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
     5341 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     5401 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     5461 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     5521 gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     5581 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     5641 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     5701 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     5761 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5821 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
     5881 cctcaggact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5941 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6001 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6421 tgatttaaca aaaatttaac gcgaatttt
//
