LOCUS       V022696                10895 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022696
VERSION     V022696
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..5542
                     /label="CC2D2A(NM_001378615)"
                     /note="CC2D2A(NM_001378615)"
                     /gene="CC2D2A"
     CDS             5582..5647
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5671..6259
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6325..6373
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6575..7003
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7017..7346
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7413..8207
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8383..8504
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8553..8569)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8577..8593
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8601..8631)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8646..8667
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8955..9543)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9714..10574)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10575..10679)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgaatcc cagggaagaa aaagtaaaaa taattacaga
      721 ggagttcatt gaaaatgatg aggatgcaga catgggaaga cagaataaga actcaaaggt
      781 tcgaagacag ccaagaaaga aacagccacc aactgctgtc cccaaggaaa tggtgtccga
      841 aaaatcccac cttggcaacc cccaggagcc tgtgcaggag gagcccaaga cccgcctcct
      901 gagtatgaca gtccggagag gcccacggag cttacctcca attccttcaa cttccagaac
      961 aggctttgca gaattttcca tgaggggacg catgagggag aaattgcaag cagcgaggtc
     1021 caaagcagaa agtgcattgc tgcaggaaat ccccactcct cggcccagac gcttacgaag
     1081 tcccagtaag aaagaattgg agactgaatt tggcacagag ccagggaaag aggtagaaag
     1141 gactcaacaa gaagttgact cccaaagtta ctcaagagtc aagttccatg attctgcacg
     1201 aaaaatcaag cctaaacccc aggttccacc tggcttccct tctgcagaag aggcctataa
     1261 cttctttact ttcaactttg atcccgaacc agaaggatca gaggaaaaac caaaagcaag
     1321 acatagagcg ggaactaatc aagaggagga ggaaggggaa gaagaagaac cacctgcaca
     1381 aggaggagga aaggaaatgg atgaggaaga actgcttaat ggtgatgatg ccgaggactt
     1441 cctattgggc ttagatcacg tggctgacga ttttgtagca gtcagacctg cagattatga
     1501 aagcatccat gatcggctgc agatggaaag agaaatgctc ttcataccca gtaggcagac
     1561 agtccctaca tataaaaagc ttcctgagaa tgtacagccc aggttcctgg aagatgaagg
     1621 cctttacacc ggggtaagac cagaggtggc acgcaccaat cagaacatca tggagaacag
     1681 attgctgatg caggaccccg aaagaagatg gtttggagat gacggcagga tcctagctct
     1741 gccaaacccc atcaagccat ttccttcaag gccgccagta ctaacacagg agcagagcat
     1801 taaggcagag cttgaaacac tgtataaaaa ggctgtaaaa tacgttcaca gtagtcagca
     1861 tgtgatcaga tctggagacc ctcctggaaa tttccaactg gacattgata tttcagggtt
     1921 aatcttcact catcatccct gttttagccg agagcatgtt ttggcagcca agctggccca
     1981 gttatatgac cagtaccttg caagacacca gagaaacaag gcgaaatttc ttactgataa
     2041 gctccaagct ttaagaaatg ctgttcagac tggccttgat ccagaaaaac ctcatcagtc
     2101 tctcgatacc atccaaaaaa ccatcaatga gtataaatct gaaattcgac aaacaagaaa
     2161 attccgtgat gctgaacaag aaaaagatag aacattgctt aagactatca taaaagtttg
     2221 gaaagagatg aaatcccttc gagagttcca gagatttaca aatactccct tgaaacttgt
     2281 tttgagaaag gaaaaagctg accagaaagc agatgaagaa gcatatgaag cagaaattca
     2341 agctgaaata agtgaactgt tagaagagca cacggaggag tacgcacaga agatggaaga
     2401 atacagaacg tcgttacaac agtggaaggc ctggaggaaa gtgcaaaggg ccaagaagaa
     2461 gaaaaggaaa caagcagcag aagaacatcc cggtgatgag attgcagagc cgtatcccga
     2521 ggaggacctt gtgaagccca gccctccaga gcccactgat cgggcagtga tagagcagga
     2581 ggtgagggag agagcagccc agagcaggag gaggccttgg gagcccacgc tggtcccgga
     2641 gctaagcctg gcaggaagcg taacacccaa tgaccagtgc cccagagcgg aggtctcgag
     2701 aagggaggat gtaaagaagc gctcagtgta cttaaaagtg ctgttcaaca acaaggaggt
     2761 gtccaggaca gtcagtcggc cactaggagc agacttccga gttcactttg ggcagatttt
     2821 caatttgcaa atagtcaact ggccggagag tttaacactt caggtctatg aaactgtcgg
     2881 acacagtagt cccaccttgc tagcagaagt gtttctgcct attcctgaga ctactgttgt
     2941 cactggaagg gctcctactg aagaagtgga gtttagcagt aatcagcatg tgacactgga
     3001 ccacgaggga gttggaagtg gagtgccctt ctcatttgaa gctgatggca gtaaccagct
     3061 gactctgatg acctcaggga aagtgtctca tagtgtggca tgggccattg gagaaaacgg
     3121 gataccttta attcctccat tgtcacagca gaacatcgga tttcggagtg ctttgaagaa
     3181 agcagatgcc atctcatcta ttggcacatc aggactgaca gacatgaaaa aattggccaa
     3241 gtgggcagca gagtccaagc tcgacccaaa tgaccccaac aatgcccctt tgatgcagct
     3301 tatctcggtt gctaccagtg gtgaatccta tgtccctgat ttctttagac tggagcagct
     3361 gcaacaggag tttaactttg tttcagatca agaattaaat agatccaaac gatttaggct
     3421 tcttcatctt agaagccaag aggtgccaga attccgaaat tataagcaag ttccagtcta
     3481 tgaccgagaa attatggaaa aggtattcca ggactatgag aaacggttac gagacagaaa
     3541 tgtaatagaa accaaggaac acatagacac ccatagggcc atagtagcca agtacctcca
     3601 gcaggttaga gaatcagtga taaatcgttt cttaattgca aaacaatatt ttcttcttgc
     3661 tgatatgata gtagaagaag aagttcccaa tatcagcatt ttgggcctaa gccttttcaa
     3721 gctggcagaa caaaagcgac cactgcggcc aaggagaaaa ggtcggaaga aggtgacagc
     3781 ccaaaacctg tctgatggag acataaagct gctggtgaac attgtgcgag cttacgacat
     3841 tccagtgagg aagccggcag tgagcaaatt ccagcagccg tcgaggtctt caaggatgtt
     3901 cagtgaaaag catgctgctt ccccaagcac gtacagccca acccacaatg ctgactaccc
     3961 cctcggccag gttttagtac gtccctttgt agaagtctct tttcaacgaa cagtttgcca
     4021 tacgactacg gctgaaggac caaaccctag ctggaatgaa gaactagaac ttccatttag
     4081 ggctcctaat ggagattata gcacagccag tctgcagtca gtgaaagatg ttgtgttcat
     4141 taacattttt gatgaagtac tgcatgatgt cttagaggat gaccgtgaaa gaggaagtgg
     4201 aatccatact cgtattgaga gacactggct gggatgtgtg aaaatgccat ttagcacaat
     4261 atatttccaa gcaaggattg atggaacatt taaaatagat attcccccag ttcttctggg
     4321 ctacagtaag gagcgaaata tgattcttga gcggggtttt gattctgtcc gaagcttaag
     4381 tgaaggctcc tacattaccc tctttattac cattgagccc cagctggttc ctggagagtc
     4441 cattcgagaa aagtttgagt ctcaggaaga tgagaaatta cttcaagcaa ctgagaagtt
     4501 tcaagctgaa tgtgccttaa agtttccaaa tcgtcagtgc cttacaacag taattgatat
     4561 aagcggaaaa actgttttta tcacacgtta tctcaaacct ttaaaccctc ctcaggagct
     4621 ccttaatgtc taccccaata atctacaggc aactgcagaa ctggtggctc gatatgtgtc
     4681 cttgattccc ttcttgcctg acactgtctc atttggtggt atctgtgacc tctggagcac
     4741 atctgatcaa tttcttgatc tcctggcagg ggatgaagaa gaacatgcag tactattgtg
     4801 taattacttt ctgtctctgg gtaagaaggc ctggctgttg atgggcaatg ctattcctga
     4861 gggtccaact gcctatgtgc taacttggga gcaaggtcgt tatttaatat ggaatccctg
     4921 cagtggacat ttttatggac aatttgatac attctgtccc ttgaaaaatg tgggctgttt
     4981 aataggtcct gacaatattt ggtttaatat tcaacgatat gaatctccac taaggataaa
     5041 ttttgatgtc accaggccca agctatggaa atctttcttt tcaagaagcc ttccatatcc
     5101 tggcctttcc agtgttcagc ctgaagagct aatttaccag cgctcagaca aagcagctgc
     5161 agctgagcta caagacagga ttgaaaaaat actaaaagaa aaaatcatgg actggaggcc
     5221 acgccatctg actcggtgga ataggtattg tacctctact ctgcgtcact tcttgcctct
     5281 gttagaaaaa agtcaaggag aagatgtaga agatgaccac agagcagaac tgctaaaaca
     5341 gctgggagac tacaggttct ctggatttcc tcttcacatg ccttattctg aagtgaagcc
     5401 tttaattgac gctgtgtata gtactggagt acataatatt gatgttccta atgttgaatt
     5461 tgctttagct gtatacatac acccataccc caaaaatgtt ttgtctgttt ggatctatgt
     5521 tgcctctctt atacgcaaca gggaattctg cagatatcca gcacagtggc ggccgctcga
     5581 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     5641 tgacaagtag tgagggcccg atatctcgac aatcaacctc tggattacaa aatttgtgaa
     5701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6061 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6241 ctttgggccg cctccccgcc tggaaacggg ggaggctaac tgaaacacgg aaggagacaa
     6301 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     6361 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6421 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6481 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca
     6541 gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6721 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     6961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7021 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7081 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7141 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7201 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7261 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7321 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7381 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7441 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7501 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7561 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7621 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7681 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7741 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7801 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7861 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7921 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7981 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8041 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8101 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8161 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8221 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8281 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8341 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8521 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8581 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8641 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8701 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8761 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8821 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9121 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     9181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9361 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9721 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9781 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9841 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9901 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9961 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10021 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10081 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10141 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10201 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10261 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10321 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10381 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10441 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10501 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10561 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10621 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10681 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10741 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10801 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10861 gcttgaccga caattgcatg aagaatctgc ttagg
//
