LOCUS       V031273                 5478 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031273
VERSION     V031273
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1625
                     /label="Bcl2(NM_177410)"
                     /note="Bcl2(NM_177410)"
                     /gene="Bcl2"
     CDS             1644..1673
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1692..1715
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1735..1751)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1762..2384
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2533..3121)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3450..3497
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3729..4523)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4558..4915)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4917..5021)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5048..5476
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcgcaagcc gggagaacag ggtatgataa ccgggagatc gtgatgaagt
     1081 acatacatta taagctgtca cagaggggct acgagtggga tgctggagat gcggacgcgg
     1141 cgcccctggg ggctgccccc acccctggca tcttctcctt ccagcctgag agcaacccaa
     1201 tgcccgctgt gcaccgggac atggctgcca ggacgtctcc tctcaggccc ctcgttgcca
     1261 ccgctgggcc tgcgctcagc cctgtgccac ctgtggtcca tctgaccctc cgccgggctg
     1321 gggatgactt ctctcgtcgc taccgtcgtg acttcgcaga gatgtccagt cagctgcacc
     1381 tgacgccctt caccgcgagg ggacgctttg ccacggtggt ggaggaactc ttcagggatg
     1441 gggtgaactg ggggaggatt gtggccttct ttgagttcgg tggggtcatg tgtgtggaga
     1501 gcgtcaacag ggagatgtca cccctggtgg acaacatcgc cctgtggatg actgagtacc
     1561 tgaaccggca tctgcacacc tggatccagg ataacggagg ctgggtaggt gcatgtctgg
     1621 ttgaaacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     1681 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     1741 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     1801 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     1861 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     1921 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     1981 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     2041 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     2101 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     2161 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     2221 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     2281 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     2341 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     2401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2941 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3241 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     3301 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     3361 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     3421 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     3481 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     3541 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     3601 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     3661 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     3721 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     3781 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     3841 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     3901 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     3961 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     4021 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4081 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4141 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4201 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4261 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4321 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4381 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4441 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4501 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     4561 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4621 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4681 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4741 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     4801 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4861 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5041 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5461 aaatttaacg cgaatttt
//
