LOCUS       V043324                 9034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043324
VERSION     V043324
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             991..5241
                     /label="BLM(NM_000057)"
                     /note="BLM(NM_000057)"
                     /gene="BLM"
     CDS             5245..5274
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(5291..5307)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    5318..5940
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(6089..6677)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    7006..7053
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(7285..8079)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(8114..8471)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(8473..8577)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      8604..9032
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgccacc atggctgctg ttcctcaaaa taatctacag
     1021 gagcaactag aacgtcactc agccagaaca cttaataata aattaagtct ttcaaaacca
     1081 aaattttcag gtttcacttt taaaaagaaa acatcttcag ataacaatgt atctgtaact
     1141 aatgtgtcag tagcaaaaac acctgtatta agaaataaag atgttaatgt taccgaagac
     1201 ttttccttca gtgaacctct acccaacacc acaaatcagc aaagggtcaa ggacttcttt
     1261 aaaaatgctc cagcaggaca ggaaacacag agaggtggat caaaatcatt attgccagat
     1321 ttcttgcaga ctccgaagga agttgtatgc actacccaaa acacaccaac tgtaaagaaa
     1381 tcccgggata ctgctctcaa gaaattagaa tttagttctt caccagattc tttaagtacc
     1441 atcaatgatt gggatgatat ggatgacttt gatacttctg agacttcaaa atcatttgtt
     1501 acaccacccc aaagtcactt tgtaagagta agcactgctc agaaatcaaa aaagggtaag
     1561 agaaactttt ttaaagcaca gctttataca acaaacacag taaagactga tttgcctcca
     1621 ccctcctctg aaagcgagca aatagatttg actgaggaac agaaggatga ctcagaatgg
     1681 ttaagcagcg atgtgatttg catcgatgat ggccccattg ctgaagtgca tataaatgaa
     1741 gatgctcagg aaagtgactc tctgaaaact catttggaag atgaaagaga taatagcgaa
     1801 aagaagaaga atttggaaga agctgaatta cattcaactg agaaagttcc atgtattgaa
     1861 tttgatgatg atgattatga tacggatttt gttccacctt ctccagaaga aattatttct
     1921 gcttcttctt cctcttcaaa atgccttagt acgttaaagg accttgacac ctctgacaga
     1981 aaagaggatg ttcttagcac atcaaaagat cttttgtcaa aacctgagaa aatgagtatg
     2041 caggagctga atccagaaac cagcacagac tgtgacgcta gacagataag tttacagcag
     2101 cagcttattc atgtgatgga gcacatctgt aaattaattg atactattcc tgatgataaa
     2161 ctgaaacttt tggattgtgg gaacgaactg cttcagcagc ggaacataag aaggaaactt
     2221 ctaacggaag tagattttaa taaaagtgat gccagtcttc ttggctcatt gtggagatac
     2281 aggcctgatt cacttgatgg ccctatggag ggtgattcct gccctacagg gaattctatg
     2341 aaggagttaa atttttcaca ccttccctca aattctgttt ctcctgggga ctgtttactg
     2401 actaccaccc taggaaagac aggattctct gccaccagga agaatctttt tgaaaggcct
     2461 ttattcaata cccatttaca gaagtccttt gtaagtagca actgggctga aacaccaaga
     2521 ctaggaaaaa aaaatgaaag ctcttatttc ccaggaaatg ttctcacaag cactgctgtg
     2581 aaagatcaga ataaacatac tgcttcaata aatgacttag aaagagaaac ccaaccttcc
     2641 tatgatattg ataattttga catagatgac tttgatgatg atgatgactg ggaagacata
     2701 atgcataatt tagcagccag caaatcttcc acagctgcct atcaacccat caaggaaggt
     2761 cggccaatta aatcagtatc agaaagactt tcctcagcca agacagactg tcttccagtg
     2821 tcatctactg ctcaaaatat aaacttctca gagtcaattc agaattatac tgacaagtca
     2881 gcacaaaatt tagcatccag aaatctgaaa catgagcgtt tccaaagtct tagttttcct
     2941 catacaaagg aaatgatgaa gatttttcat aaaaaatttg gcctgcataa ttttagaact
     3001 aatcagctag aggcgatcaa tgctgcactg cttggtgaag actgttttat cctgatgccg
     3061 actggaggtg gtaagagttt gtgttaccag ctccctgcct gtgtttctcc tggggtcact
     3121 gttgtcattt ctcccttgag atcacttatc gtagatcaag tccaaaagct gacttccttg
     3181 gatattccag ctacatatct gacaggtgat aagactgact cagaagctac aaatatttac
     3241 ctccagttat caaaaaaaga cccaatcata aaacttctat atgtcactcc agaaaagatc
     3301 tgtgcaagta acagactcat ttctactctg gagaatctct atgagaggaa gctcttggca
     3361 cgttttgtta ttgatgaagc acattgtgtc agtcagtggg gacatgattt tcgtcaagat
     3421 tacaaaagaa tgaatatgct tcgccagaag tttccttctg ttccggtgat ggctcttacg
     3481 gccacagcta atcccagggt acagaaggac atcctgactc agctgaagat tctcagacct
     3541 caggtgttta gcatgagctt taacagacat aatctgaaat actatgtatt accgaaaaag
     3601 cctaaaaagg tggcatttga ttgcctagaa tggatcagaa agcaccaccc atatgattca
     3661 gggataattt actgcctctc caggcgagaa tgtgacacca tggctgacac gttacagaga
     3721 gatgggctcg ctgctcttgc ttaccatgct ggcctcagtg attctgccag agatgaagtg
     3781 cagcagaagt ggattaatca ggatggctgt caggttatct gtgctacaat tgcatttgga
     3841 atggggattg acaaaccgga cgtgcgattt gtgattcatg catctctccc taaatctgtg
     3901 gagggttact accaagaatc tggcagagct ggaagagatg gggaaatatc tcactgcctg
     3961 cttttctata cctatcatga tgtgaccaga ctgaaaagac ttataatgat ggaaaaagat
     4021 ggaaaccatc atacaagaga aactcacttc aataatttgt atagcatggt acattactgt
     4081 gaaaatataa cggaatgcag gagaatacag cttttggcct actttggtga aaatggattt
     4141 aatcctgatt tttgtaagaa acacccagat gtttcttgtg ataattgctg taaaacaaag
     4201 gattataaaa caagagatgt gactgacgat gtgaaaagta ttgtaagatt tgttcaagaa
     4261 catagttcat cacaaggaat gagaaatata aaacatgtag gtccttctgg aagatttact
     4321 atgaatatgc tggtcgacat tttcttgggg agtaagagtg caaaaatcca gtcaggtata
     4381 tttggaaaag gatctgctta ttcacgacac aatgccgaaa gactttttaa aaagctgata
     4441 cttgacaaga ttttggatga agacttatat atcaatgcca atgaccaggc gatcgcttat
     4501 gtgatgctcg gaaataaagc ccaaactgta ctaaatggca atttaaaggt agactttatg
     4561 gaaacagaaa attccagcag tgtgaaaaaa caaaaagcgt tagtagcaaa agtgtctcag
     4621 agggaagaga tggttaaaaa atgtcttgga gaacttacag aagtctgcaa atctctgggg
     4681 aaagtttttg gtgtccatta cttcaatatt tttaataccg tcactctcaa gaagcttgca
     4741 gaatctttat cttctgatcc tgaggttttg cttcaaattg atggtgttac tgaagacaaa
     4801 ctggaaaaat atggtgcgga agtgatttca gtattacaga aatactctga atggacatcg
     4861 ccagctgaag acagttcccc agggataagc ctgtccagca gcagaggccc cggaagaagt
     4921 gccgctgagg agctcgacga ggaaataccc gtatcttccc actactttgc aagtaaaacc
     4981 agaaatgaaa ggaagaggaa aaagatgcca gcctcccaaa ggtctaagag gagaaaaact
     5041 gcttccagtg gttccaaggc aaaggggggg tctgccacat gtagaaagat atcttccaaa
     5101 acgaaatcct ccagcatcat tggatccagt tcagcctcac atacttctca agcgacatca
     5161 ggagccaata gcaaattggg gattatggct ccaccgaagc ctataaatag accgtttctt
     5221 aagccttcat atgcattctc actcgagcag aaactcatct cagaagagga tctgtaaacg
     5281 gccggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc
     5341 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     5401 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     5461 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     5521 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     5581 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     5641 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     5701 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     5761 acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca
     5821 ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag
     5881 ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt
     5941 gggtacgcgg ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     6841 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     6901 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     6961 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     7021 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     7081 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     7141 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     7201 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     7261 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     7321 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     7381 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac
     7441 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     7501 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     7561 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     7621 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     7681 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     7741 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     7801 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     7861 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     7921 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     7981 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     8041 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     8101 ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     8161 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     8221 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     8281 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     8341 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     8401 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
     8461 ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8581 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9001 tgagctgatt taacaaaaat ttaacgcgaa tttt
//
