LOCUS       V030691                 5448 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030691
VERSION     V030691
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1595
                     /label="Apod(NM_001301354)"
                     /note="Apod(NM_001301354)"
                     /gene="Apod"
     CDS             1614..1643
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1662..1685
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1705..1721)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1732..2354
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2503..3091)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3420..3467
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3699..4493)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4528..4885)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4887..4991)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5018..5446
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggtgaccatg ctgatgttcc tggccacgct ggcgggtctc ttcaccacag
     1081 ccaaaggaca aaatttccat cttgggaaat gcccgtctcc tcctgtgcaa gagaattttg
     1141 acgtgaaaaa gtatcttgga agatggtacg aaattgagaa gatcccagcg agctttgaga
     1201 aaggaaactg cattcaagcc aactactcgc tgatggagaa cggaaacatc gaagtgctaa
     1261 acaaggagct gagtcctgat ggaaccatga accaagtaaa gggtgaagcc aaacagagca
     1321 acgtctcaga gccagccaag ctggaagtcc agttcttccc gttgatgcca ccggcaccct
     1381 actggatcct ggccactgat tatgaaaact atgccctcgt gtactcctgc accaccttct
     1441 tctggctctt ccatgtggat tttgtttgga ttcttggaag aaatccttat ctccctccag
     1501 aaacaataac ctacctaaaa gatatcctta cttctaatgg catcgacatc gaaaaaatga
     1561 caacaacaga tcaagcgaac tgcccggact tcctgacgcg tacgcggccg ctcgagcaga
     1621 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     1681 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     1741 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     1801 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     1861 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     1921 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     1981 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2041 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2101 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2161 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     2221 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     2281 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     2341 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     2401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2941 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3241 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3301 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3361 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3421 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3481 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     3541 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3601 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     3661 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     3721 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     3781 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     3841 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     3901 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     3961 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4021 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4081 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     4141 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     4201 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4261 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4321 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4381 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4441 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4501 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     4561 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4621 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     4681 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4741 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     4801 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     4861 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     5041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5281 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5341 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5401 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
